Jarid2 (NM_001205044) Mouse Untagged Clone

CAT#: MC229497

Jarid2 (untagged) - Mouse jumonji, AT rich interactive domain 2 (Jarid2), transcript variant 3



  "NM_001205044" in other vectors (1)

CNY 11,880.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Jmj; jumonji
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229497 representing NM_001205044
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAAGGAAAGACCCAAGAGGAATATCATTCAGAAGAAATACGATGACAGCGATGGGATCCCGTGGT
CAGAAGAGAGAGTTGTACGAAAAGTCCTGTATTTGTCCCTAAAGGAATTCAAGAATGCACAGAAAAGGCA
GCATGGGGAAGGCCTTGCCGGGAGCCTGAAGGCGGTAAATGGGCTTCTTGGTAATGCCCAGGCTAAGGCA
CTAGGACCAGCCTCAGAGCAGTCAGAGAACGAGAAGGATGATGCCTCCCAAGTGTCCTCTACTAGCAACG
ATGTTAGTTCTTCAGATTTTGAAGAAGGGCCGTCGAGGAAAAGGCCCAGGCTGCAAGCACAAAGGAAGTT
TGCTCAATCTCAGCCGAATAGTCCCAGCACAACTCCAGTGAAGATAGTGGAGCCTTTGCTACCCCCGCCA
GCTACTCAAATTTCTGACCTCTCTAAAAGGAAGCCTAAGACAGAAGACTTTCTTACCTTTCTCTGCCTTC
GAGGTTCTCCTGCGCTGCCCAACAGTATGGTATATTTTGGAAGCTCTCAGGATGAGGAGGATGTCGAAGA
GGAAGATGATGAGACGGAAGATGTCAAAGCAACCACCAACAATGCTTCATCTTCGTGCCAGTCAACCCCC
AGGAAAGGAAAAACCCATAAGCATGTACACAACGGGCACGTTTTCAATGGCTCCAGTAGGTCAGCACGGG
AGAAAGAGCCTGCTCACAAACACAGAAGCAAAGAGGCCACTCCGGGGAAGGAGAAGCACAGCGAGCCCAG
GGCAGACAGCCGGAGAGAACAGGCTTCAGGGGCTCAGCCCACGGCCGCCTCGGCTGCGGCTTCCTCTGCC
AAGGGACTTGCTGCCAACCACCAACCTCCCCCTTCGCATCGGTCTGCTCAGGACTTACGGAAACAGGTGT
CTAAGGTAAATGGAGTCACTCGAATGTCATCTCTGGGTGCAGGTACAAACAGTGCCAAAAAGATCCGAGA
AGTCAGACCTTCACCATCCAAAACTGTGAAGTACACTGCCACAGTGACCAAGGGGACTGTCACATACACC
AAAGCCAAGAGAGAACTGGTCAAGGAAACCAAACCCAACCACCACAAACCCAGTTCAGCTGTCAACCACA
CAATCTCAGGGAAAACTGAAAGTAGCAATGCAAAAACCCGCAAACAGGTGCTATCCCTTGGGGGGGCGTC
CAAGTCCACCGGGCCTGCTGCCAGTGGCCTCAAGGCCAGCAGCAGGTTGAACCCAAAGTCATGCACTAAG
GAGGTGGGGGGGCGGCAGCTGAGGGAGGGGCTGCGGAATTCCAAAAGGAGACTGGAAGAGGCACAGCAAG
TGGACAAGCCGCAGTCACCCCCCAAGAAGATGAAGGGGGTGGCAGGCAATGCTGAAGCCCCTGGCAAAAA
GGCCTCAGCGGCTTCAGGAGAAAAGTCTCTGCTGAATGGACACGTGAAGAAGGAAGTGCCCGAGCGAAGT
TTGGAAAGGAATCGGCCAAAGCGGGCCGCGGCTGGCAAGAATATGCTGGGCAAACAAGCACATGGCAAGA
CAGAGGGCACCCCCTGTGAAAATCGTTCTACCTCGCAACCCGAGTCCTCGCACAAGCCGCATGACCCACA
GGGCAAGCCAGAGAAGGGGAGCGGCAAGTCTGGGTGGGCAGCGATGGACGAGATCCCTGTCCTCAGGCCC
TCCGCCAAGGAGTTCCACGACCCGCTCATCTACATCGAGTCAGTACGTGCTCAGGTGGAGAAGTACGGGA
TGTGTCGCGTGATTCCCCCTCCAGACTGGCGGCCAGAGTGCAAGCTCAACGATGAGATGCGCTTTGTCAC
GCAGATCCAGCACATCCATAAGCTGGGCCGGCGCTGGGGCCCCAACGTGCAGCGGCTAGCCTGCATAAAG
AAGCACCTCAGATCTCAGGGCATCACCATGGATGAGCTCCCCCTCATAGGAGGCTGTGAGCTCGACCTGG
CCTGCTTTTTCCGGCTGATTAATGAGATGGGCGGCATGCAGCAAGTGACTGACCTCAAAAAATGGAACAA
ACTAGCAGACATGCTGCGCATTCCCAAAACTGCCCAGGACCGGCTGGCCAAGCTGCAGGAGGCCTACTGC
CAGTACCTGCTTTCCTATGACTCCCTGTCCCCCGAGGAGCACCGACGGCTGGAGAAGGAGGTGCTGATGG
AGAAGGAGATCCTGGAAAAGCGCAAAGGGCCATTAGAGGGGCACACGGAGAGCGACCACCACAAGTTCCA
CTCCCTGCCCCGCTTTGAGCCCAAGAATGGGCTCGTCCACGGTGTCACACCTAGGAATGGATTCCGTAGC
AAGCTCAAGGAGGTGGGCCGGGCCCCACTGAAGACGGGCCGGCGGCGGCTGTTCGCTCAGGAAAAAGAAG
TGGTCAAAGAGGAGGAAGAAGACAAAGGTGTCCTCAATGACTTCCACAAGTGCATCTATAAGGGAAGATC
TGTTTCTCTAACAACTTTTTATCGAACAGCAAGAAACATCATGAACATGTGTTTCAGCAAGGAGCCCGCA
CCAGCAGAGATTGAGCAAGAGTACTGGAGGTTAGTGGAAGAGAAGGACTGTCATGTGGCAGTTCACTGCG
GAAAGGTGGACACTAATACCCATGGCAGCGGGTTCCCGGTGGGAAAATCAGAACCCTTTTCAAGGCATGG
ATGGAACCTCACTGTCCTCCCAAATAACACAGGGTCCATCCTGCGTCACCTTGGTGCTGTGCCTGGAGTG
ACTATTCCCTGGCTAAATATTGGCATGGTCTTTTCTACCTCATGCTGGTCTCGAGACCAAAATCACCTTC
CATATATTGACTACTTACACACTGGTGCTGACTGCATTTGGTATTGCATTCCTGCTGAGGAGGAGAACAA
GCTGGAGGACGTAGTCCACACTCTGCTGCAGGGCAATGGTACCCCAGGGCTGCAGATGCTGGAGAGCAAT
GTAATGATCTCCCCAGAGGTGCTGTGCAAGAAGGGGATCAAGGTCCACAGGACTGTACAGCAGAGTGGGC
AGTTTGTCGTCTGCTTCCCGGGATCCTTTGTGTCCAAAGTATGTTGTGGCTACAACGTCTCAGAGACAGT
GCACTTTGCTACCACCCAGTGGACAAGCATGGGCTTCGAGACTGCCAAGGAGATGAAGCGCCGCCATATA
GCTAAGCCATTCTCCATGGAGAAGTTGCTCTACCAGATTGCACAAGCAGAAGCAAAAAAGGAAAATGGTC
CCACCCTCAGTACCATCTCAGCCCTTCTGGATGAGCTCAGGGATACAGAGCTGCGGCAGCGCAGGCTGTT
GTTCGAGGCTGGCCTTCACTCTTCTGCACGCTATGGCAGCCACGACGGCAACAGCACAGTGGCGGATGGG
AAGAAAAAGCCTCGAAAGTGGCTACAGCTGGAAACTTCAGAAAGGAGGTGCCAGATCTGCCAGCACTTGT
GCTACCTGTCCATGGTGGTACAGGAGAATGAGAATGTCGTGTTCTGCCTGGAGTGTGCACTGCGCCATGT
GGAGAAGCAGAAGTCTTGCCGTGGGCTGAAGCTGATGTACCGCTACGATGAGGAGCAGATCATCAGTTTG
GTCAACCAGATATGTGGCAAAGTGTCTGGCAAACACGGTGGCATCGAGAACTGTCTCAACAAACCCACAC
CAAAAAGAGGTCCCCGCAAGAGAGCGACCGTGGATGTGCCGCCATCTCGGCTCCCATCCTCATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001205044
Insert Size 3705 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001205044.1, NP_001191973.1
RefSeq Size 5670 bp
RefSeq ORF 3705 bp
Locus ID 16468
UniProt ID Q62315
Gene Summary Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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