Jarid2 (NM_001205044) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Jmj; jumonji
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229497 representing NM_001205044 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAGGAAAGACCCAAGAGGAATATCATTCAGAAGAAATACGATGACAGCGATGGGATCCCGTGGT CAGAAGAGAGAGTTGTACGAAAAGTCCTGTATTTGTCCCTAAAGGAATTCAAGAATGCACAGAAAAGGCA GCATGGGGAAGGCCTTGCCGGGAGCCTGAAGGCGGTAAATGGGCTTCTTGGTAATGCCCAGGCTAAGGCA CTAGGACCAGCCTCAGAGCAGTCAGAGAACGAGAAGGATGATGCCTCCCAAGTGTCCTCTACTAGCAACG ATGTTAGTTCTTCAGATTTTGAAGAAGGGCCGTCGAGGAAAAGGCCCAGGCTGCAAGCACAAAGGAAGTT TGCTCAATCTCAGCCGAATAGTCCCAGCACAACTCCAGTGAAGATAGTGGAGCCTTTGCTACCCCCGCCA GCTACTCAAATTTCTGACCTCTCTAAAAGGAAGCCTAAGACAGAAGACTTTCTTACCTTTCTCTGCCTTC GAGGTTCTCCTGCGCTGCCCAACAGTATGGTATATTTTGGAAGCTCTCAGGATGAGGAGGATGTCGAAGA GGAAGATGATGAGACGGAAGATGTCAAAGCAACCACCAACAATGCTTCATCTTCGTGCCAGTCAACCCCC AGGAAAGGAAAAACCCATAAGCATGTACACAACGGGCACGTTTTCAATGGCTCCAGTAGGTCAGCACGGG AGAAAGAGCCTGCTCACAAACACAGAAGCAAAGAGGCCACTCCGGGGAAGGAGAAGCACAGCGAGCCCAG GGCAGACAGCCGGAGAGAACAGGCTTCAGGGGCTCAGCCCACGGCCGCCTCGGCTGCGGCTTCCTCTGCC AAGGGACTTGCTGCCAACCACCAACCTCCCCCTTCGCATCGGTCTGCTCAGGACTTACGGAAACAGGTGT CTAAGGTAAATGGAGTCACTCGAATGTCATCTCTGGGTGCAGGTACAAACAGTGCCAAAAAGATCCGAGA AGTCAGACCTTCACCATCCAAAACTGTGAAGTACACTGCCACAGTGACCAAGGGGACTGTCACATACACC AAAGCCAAGAGAGAACTGGTCAAGGAAACCAAACCCAACCACCACAAACCCAGTTCAGCTGTCAACCACA CAATCTCAGGGAAAACTGAAAGTAGCAATGCAAAAACCCGCAAACAGGTGCTATCCCTTGGGGGGGCGTC CAAGTCCACCGGGCCTGCTGCCAGTGGCCTCAAGGCCAGCAGCAGGTTGAACCCAAAGTCATGCACTAAG GAGGTGGGGGGGCGGCAGCTGAGGGAGGGGCTGCGGAATTCCAAAAGGAGACTGGAAGAGGCACAGCAAG TGGACAAGCCGCAGTCACCCCCCAAGAAGATGAAGGGGGTGGCAGGCAATGCTGAAGCCCCTGGCAAAAA GGCCTCAGCGGCTTCAGGAGAAAAGTCTCTGCTGAATGGACACGTGAAGAAGGAAGTGCCCGAGCGAAGT TTGGAAAGGAATCGGCCAAAGCGGGCCGCGGCTGGCAAGAATATGCTGGGCAAACAAGCACATGGCAAGA CAGAGGGCACCCCCTGTGAAAATCGTTCTACCTCGCAACCCGAGTCCTCGCACAAGCCGCATGACCCACA GGGCAAGCCAGAGAAGGGGAGCGGCAAGTCTGGGTGGGCAGCGATGGACGAGATCCCTGTCCTCAGGCCC TCCGCCAAGGAGTTCCACGACCCGCTCATCTACATCGAGTCAGTACGTGCTCAGGTGGAGAAGTACGGGA TGTGTCGCGTGATTCCCCCTCCAGACTGGCGGCCAGAGTGCAAGCTCAACGATGAGATGCGCTTTGTCAC GCAGATCCAGCACATCCATAAGCTGGGCCGGCGCTGGGGCCCCAACGTGCAGCGGCTAGCCTGCATAAAG AAGCACCTCAGATCTCAGGGCATCACCATGGATGAGCTCCCCCTCATAGGAGGCTGTGAGCTCGACCTGG CCTGCTTTTTCCGGCTGATTAATGAGATGGGCGGCATGCAGCAAGTGACTGACCTCAAAAAATGGAACAA ACTAGCAGACATGCTGCGCATTCCCAAAACTGCCCAGGACCGGCTGGCCAAGCTGCAGGAGGCCTACTGC CAGTACCTGCTTTCCTATGACTCCCTGTCCCCCGAGGAGCACCGACGGCTGGAGAAGGAGGTGCTGATGG AGAAGGAGATCCTGGAAAAGCGCAAAGGGCCATTAGAGGGGCACACGGAGAGCGACCACCACAAGTTCCA CTCCCTGCCCCGCTTTGAGCCCAAGAATGGGCTCGTCCACGGTGTCACACCTAGGAATGGATTCCGTAGC AAGCTCAAGGAGGTGGGCCGGGCCCCACTGAAGACGGGCCGGCGGCGGCTGTTCGCTCAGGAAAAAGAAG TGGTCAAAGAGGAGGAAGAAGACAAAGGTGTCCTCAATGACTTCCACAAGTGCATCTATAAGGGAAGATC TGTTTCTCTAACAACTTTTTATCGAACAGCAAGAAACATCATGAACATGTGTTTCAGCAAGGAGCCCGCA CCAGCAGAGATTGAGCAAGAGTACTGGAGGTTAGTGGAAGAGAAGGACTGTCATGTGGCAGTTCACTGCG GAAAGGTGGACACTAATACCCATGGCAGCGGGTTCCCGGTGGGAAAATCAGAACCCTTTTCAAGGCATGG ATGGAACCTCACTGTCCTCCCAAATAACACAGGGTCCATCCTGCGTCACCTTGGTGCTGTGCCTGGAGTG ACTATTCCCTGGCTAAATATTGGCATGGTCTTTTCTACCTCATGCTGGTCTCGAGACCAAAATCACCTTC CATATATTGACTACTTACACACTGGTGCTGACTGCATTTGGTATTGCATTCCTGCTGAGGAGGAGAACAA GCTGGAGGACGTAGTCCACACTCTGCTGCAGGGCAATGGTACCCCAGGGCTGCAGATGCTGGAGAGCAAT GTAATGATCTCCCCAGAGGTGCTGTGCAAGAAGGGGATCAAGGTCCACAGGACTGTACAGCAGAGTGGGC AGTTTGTCGTCTGCTTCCCGGGATCCTTTGTGTCCAAAGTATGTTGTGGCTACAACGTCTCAGAGACAGT GCACTTTGCTACCACCCAGTGGACAAGCATGGGCTTCGAGACTGCCAAGGAGATGAAGCGCCGCCATATA GCTAAGCCATTCTCCATGGAGAAGTTGCTCTACCAGATTGCACAAGCAGAAGCAAAAAAGGAAAATGGTC CCACCCTCAGTACCATCTCAGCCCTTCTGGATGAGCTCAGGGATACAGAGCTGCGGCAGCGCAGGCTGTT GTTCGAGGCTGGCCTTCACTCTTCTGCACGCTATGGCAGCCACGACGGCAACAGCACAGTGGCGGATGGG AAGAAAAAGCCTCGAAAGTGGCTACAGCTGGAAACTTCAGAAAGGAGGTGCCAGATCTGCCAGCACTTGT GCTACCTGTCCATGGTGGTACAGGAGAATGAGAATGTCGTGTTCTGCCTGGAGTGTGCACTGCGCCATGT GGAGAAGCAGAAGTCTTGCCGTGGGCTGAAGCTGATGTACCGCTACGATGAGGAGCAGATCATCAGTTTG GTCAACCAGATATGTGGCAAAGTGTCTGGCAAACACGGTGGCATCGAGAACTGTCTCAACAAACCCACAC CAAAAAGAGGTCCCCGCAAGAGAGCGACCGTGGATGTGCCGCCATCTCGGCTCCCATCCTCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205044
Insert Size	3705 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205044.1, NP_001191973.1
RefSeq Size	5670 bp
RefSeq ORF	3705 bp
Locus ID	16468
UniProt ID	Q62315
Gene Summary	Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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