Zeb2 (NM_001289521) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9130203F04Rik; D130016B08Rik; SIP1; Zfhx1b; Zfx1b; Zfxh1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229481 representing NM_001289521 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCAGCCGATCATGGCGGATGGCCCCCGGTGCAAGAGGCGCAAACAAGCCAATCCCAGGAGGAAAA ACGTGGTGAACTATGACAACGTAGTGGACGCAGGCTCGGAGACAGATGAAGAGGACAAGCTTCACATTGC TGAAGATGATAGCCTTGCAAACCCTCTGGACCAGGACACCAGCCCAGCTAGCATGCCCAACCATGAGTCC TCCCCACACATGAGCCAAGGGCTGCTACCAAGAGAGGAAGAAGAGGAGGAGCTAAGGGAGAGTGTTGTGG AGCACAGCTGGCACAGTGGCGAGATTCTGCAAGCCTCTGTAGCCGGTCCAGAAGAAATGAAGGAAGATTA TGATGCAATGGGGCCAGAAGCCACGATCCAGACCACAATTAACAATGGTACAGTTAAGAATGCAAATTGC ACATCAGACTTTGAGGAATACTTTGCCAAAAGAAAACTGGAGGAAAGAGATGGCCACGCAGTGAGCATCG AAGAGTACCTTCAGCGAAGCGACACGGCCATTATTTACCCAGAAGCCCCTGAGGAGCTGTCTCGCCTTGG CACGCCAGAGGCCAACGGGCAAGAAGAAAATGACCTGCCACCTGGAACTCCAGATGCTTTTGCCCAACTG CTGACCTGCCCCTACTGCGACCGCGGCTACAAGCGCTTGACATCGCTGAAGGAGCACATCAAGTACCGCC ACGAGAAGAATGAAGAGAACTTTTCCTGCCCTCTTTGTAGCTACACGTTCGCCTACCGCACCCAGCTCGA GAGGCATATGGTGACGCACAAGCCAGGGACAGATCAGCACCAAATGCTAACCCAAGGAGCAGGTAACCGC AAGTTCAAGTGCACGGAGTGTGGCAAGGCCTTCAAGTACAAGCACCACCTGAAAGAACACCTGAGAATTC ACAGTGGTGAAAAACCTTACGAATGCCCAAACTGCAAGAAACGCTTCTCTCATTCTGGGTCCTACAGTTC ACATATCAGCAGCAAGAAATGTATTGGTTTAATATCAGTAAATGGCCGAATGAGAAACAATATCAAGACG GGTTCTTCCCCTAATTCTGTTTCTTCTTCTCCTACTAACTCAGCCATTACTCAGTTAAGGAACAAGTTGG AAAATGGAAAACCACTTAGCATGTCTGAGCAGACAGGCTTACTTAAGATTAAAACAGAACCACTAGACTT CAATGACTATAAAGTTCTTATGGCAACACATGGGTTTAGTGGCAGCAGTCCCTTTATGAACGGTGGGCTT GGAGCCACCAGCCCTTTAGGTGTACACCCATCTGCTCAGAGTCCAATGCAGCACTTAGGTGTAGGGATGG AAGCCCCTTTACTTGGATTTCCCACTATGAATAGTAACTTGAGTGAGGTACAAAAGGTTCTACAGATTGT GGACAATACGGTTTCTAGGCAAAAGATGGACTGCAAGACGGAAGACATTTCAAAGTTGAAAGGTTATCAC ATGAAGGATCCATGTTCTCAGCCAGAAGAACAAGGGGTAACTTCTCCCAATATTCCCCCTGTCGGTCTTC CAGTAGTGAGTCATAACGGTGCCACTAAAAGTATTATTGACTATACCTTAGAGAAAGTCAATGAAGCCAA AGCTTGCCTCCAGAGCTTGACCACCGACTCAAGGAGACAGATCAGTAACATAAAGAAAGAGAAGTTGCGT ACTTTGATAGATTTGGTCACTGATGATAAAATGATTGAGAACCACAGCATATCCACTCCATTTTCATGCC AGTTCTGTAAAGAAAGCTTCCCGGGCCCTATTCCCCTGCATCAGCATGAACGATACCTGTGTAAGATGAA TGAAGAGATCAAGGCAGTCCTGCAACCTCATGAAAACATAGTCCCCAACAAAGCTGGAGTTTTTGTTGAT AATAAAGCCCTCCTCTTGTCATCTGTACTTTCCGAGAAAGGACTGACAAGCCCCATCAACCCATACAAGG ACCACATGTCTGTACTGAAAGCATACTATGCTATGAACATGGAGCCCAACTCTGATGAACTGCTGAAAAT CTCCATTGCTGTGGGCCTTCCTCAGGAATTTGTGAAGGAATGGTTTGAGCAAAGAAAAGTCTACCAGTAT TCGAATTCCAGGTCACCATCACTGGAAAGGACCTCCAAGCCGTTAGCTCCCAACAGTAACCCCACCACAA AAGACTCTTTGTTACCCAGGTCTCCTGTAAAACCTATGGACTCCATCACATCGCCATCTATAGCAGAACT CCACAACAGTGTTACGAGTTGTGATCCTCCTCTCAGGCTAACAAAATCTTCCCATTTCACCAATATTAAA GCAGTTGATAAACTGGACCACTCGAGGAGTAATACTCCTTCTCCTTTAAATCTTTCCTCCACATCTTCTA AAAACTCCCACAGTAGCTCGTACACTCCAAATAGCTTCTCTTCCGAGGAGCTGCAGGCTGAGCCGTTGGA CCTGTCATTACCAAAACAAATGAGAGAACCCAAAGGTATTATAGCCACAAAGAACAAAACAAAAGCTACT AGCATAAACTTAGACCACAACAGTGTTTCTTCATCGTCTGAGAATTCAGATGAGCCTCTGAATTTGACTT TTATCAAGAAAGAGTTTTCAAATTCTAATAACCTGGACAATAAAAGCAACAACCCTGTGTTCGGCATGAA CCCATTTAGTGCCAAGCCTTTATACACCCCTCTTCCACCACAGAGCGCATTTCCCCCTGCCACTTTCATG CCACCAGTCCAGACCAGCATCCCCGGGCTACGACCATACCCAGGACTGGATCAGATGAGCTTCCTACCGC ATATGGCCTATACCTACCCAACGGGAGCAGCTACCTTTGCTGATATGCAGCAAAGGAGGAAATACCAGAG GAAACAAGGATTTCAGGGAGACTTGCTGGATGGAGCACAAGACTACATGTCAGGCCTAGATGACATGACA GACTCCGATTCCTGTCTGTCTCGAAAGAAGATAAAGAAGACAGAAAGTGGCATGTATGCATGTGACTTAT GTGACAAGACATTCCAGAAAAGCAGTTCCCTTCTGCGACATAAATACGAACACACAGGAAAGAGACCACA CCAGTGTCAGATTTGTAAGAAAGCGTTCAAACACAAACACCACCTTATCGAGCACTCGAGGCTGCACTCG GGCGAGAAGCCCTATCAGTGTGACAAATGTGGCAAGCGCTTCTCACACTCGGGCTCCTACTCGCAGCACA TGAATCACAGGTACTCCTACTGCAAGCGGGAGGCGGAGGAGCGGGAAGCAGCCGAGCGCGAGGCGCGAGA GAAAGGGCACTTGGAACCCACCGAGCTGCTGATGAACCGGGCTTACCTGCAGAGCATCACCCCTCAGGGG TACTCTGACTCGGAGGAGAGGGAGAGCATGCCGAGGGATGGCGAGAGCGAGAAGGAGCACGAGAAGGAGG GCGAGGAGGGTTATGGGAAGCTGCGGAGAAGGGACGGCGACGAGGAGGAAGAGGAGGAAGAGGAAGAAAG TGAAAATAAAAGTATGGATACGGATCCCGAAACGATACGGGATGAGGAAGAGACTGGGGATCACTCGATG GACGACAGTTCAGAGGATGGGAAAATGGAAACCAAATCAGACCACGAGGAAGACAATATGGAAGATGGCA TGGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289521
Insert Size	3648 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289521.1, NP_001276450.1
RefSeq Size	8898 bp
RefSeq ORF	3648 bp
Locus ID	24136
UniProt ID	Q9R0G7
Gene Summary	Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 4. Variants 2-7 all encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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