Wapl (NM_001301330) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A530089A20Rik; BC037674; DIF-2; FOE; W; Wapal
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229463 representing NM_001301330 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCCAGATTTGGAAAAACTTACAGTAGGAAAGGAGGAAATGGCAGTTCAAAATTTGATGAAGTTT TTTCCAACAAACGGACTACTCTTAGTACAAAATGGGGTGAGACCACATTTATGGCTAAATTAGGGCAGAA GAGGCCCAATTTCAAACCAGATATTCAAGAAATTCCGAAGAAACCTAAAGTAGAAGAAGAAGATACTGGA GATCCCTTTGGTTTTGATAGTGATGATGAGTCTCTACCTGTTTCTTCAAAAAATTTAGCCCAGGGTAAGG GTTCATCTTACTCAGAATCTAGTGAGGCTGCTCAGCTGGAAGAAGTCACTTCTGTATTTGAAGCTAATAG CAAATGTAGTCATGTGGTGGGTGAAGACAGTTTTGCTTCCGACAGATGCTTACTTGTGGAGGATACTTTA ATTGGGAAAGAGAAGAGCATAAGTAGAATTCCAGAAGACAACGCAAACAAAAGTAGTTGCACTAAGTTGC TAACTTCAGATAAAGTGGAGAATTTTAGTGAAGAACATGAAAAAAATAGTCACCACTTTCACAAAAATGC TGAAGATAGTACTAAGAAACCCAATGCAGAAACCGCAGTGGCTTCTGAATATAAAGCTGATGAAACTAAA GAAACAAATGATACTTGGAACTCCCAGTCTGGAAAAAGAACAGAGTCTCCATCTGAAAGTTGTCCAGTCA AAGGATCTGTAAGAACTGGTTTATATGAATGGGATAATGATTTTGAAGATATCAGGTCAGAAGACTGTAT TTTAAGTTTGGATAATGAGTCTCTTTTGGAGATGAAAGACGAGGATTTAAAAAATCGGATTGGAGGATTG GAAAATCTAAATGAAACCTTTGAAGAAGATATCATACAAAGTGTTCTTAGGCCAAGCAACTGTAGGACGT ACTGTAGGGCCAATAAAGCGAGATCCTCACAGGGAGCATCAAATTTTGATAAGCTAATGGATGGCACCAG TCAGTCCTTAGCCAAAGCAAACAGTGAATCAAGTAAAGATGGCCTGAATCAGGCAAAGAAAGGTAGTGCA AGTTGTGGGACCAGTTTTCGAGGAACAGTTGGACGGACTAGAGATTACACTGTTTTACATCCATCTTGCT TGTCAGTGTGTAATGTTACCATCCAGGATACTATGGAACGGAGTATGGATGAGTTCACCGCATCCACTCC TGCAGATTTAGGAGAGGCTGGCCGGCTCAGAAAAAAGGCAGATATTGCAACCTCCAAGACCACTACTAGA TTTCGACCTAGTAATACTAAATCCAAAAAGGATGTTAAACTTGAATTTTTTGGTTTTGAAGATCATGATG AGACAGGAGGTGATGAAGGGGGTTCTGGAAGTTCTAATTACAAAATTAAATATTTTGGCTTTGACGATCT CAGCGAAAGTGAAGATGATGATGATGACGACTGTCAAGTGGAAAGAAAGAAAGACAAAAAAAGAACTAAA ACAGCTCCATCACCTTCCCAGCAGCCTCCTCCTGAAAGCAGCGACAATTCCCAGGATAGTCAGTCTAGTA CTAATAATGCAGAGGACTTGCCTGGTGTGCCTGAGAGTGTGAAGAAGCCCATAAGTAAACAAGGAGATAA ATCCAAGGAAAATACCAGAAAGATTTTTAGTGGCCCCAAACGGTCACCTACAAAAGCTGTATATAATGCC AGGCATTGGAACCATCCAGACTCGGAAGAATTGCCTGGACCACCAATAGCAAAACCTCAGCGTGTCACAG TGAGGCTGTCTTCAAAGGAACCAAATCAAAAAGATGATGGAGTTTTTAAGGCTCCTGCACCACCACTCAA AGTGATAAAAACTGTGACAATACCTACTCAGCCCTACCAAGAAATAGTTACTGCACTGAAATGCAGAAAA GAAGACAAAGAATTATATACTGTTGTTCAGCACGTGAAACACTTCAATGATGTGGTGGAATTTGGTGAAA ATCAAGAGTTCACTGATGACATTGAATACTTGTTAAGTGGCTTAAAGAGTACTCAGCCTCTAAACACACG TTGCCTTAGTGTTATCAGCTTAGCTACTAAATGTGCCATGCCCAGTTTTCGGATGCATCTGAGGGCACAT GGGATGGTTGCAATGGTCTTTAAAACTTTGGATGATTCCCAGCATCATCAGAATCTGTCCCTCTGTACAG CTGCTCTCATGTACATATTGAGTAGAGACCGTTTGAACATGGATCTTGATAGGGCCAGCCTAGATCTCAT GATTCGGCTTTTGGAGTTGGAACAAGATGCCTCTTCAGCTAAGCTACTGAATGAAAAAGACATGAACAAG ATCAAAGAAAAGATCCGAAGACTCTGTGAAACTGTGCACAACAAGCATCTTGATCTAGAAAACATAACGA CTGGTCATTTAGCTATGGAGACATTGCTGTCCCTCACTTCCAAACGAGCAGGAGATTGGTTTAAAGAAGA GCTCCGACTTCTGGGTGGTCTGGATCATATTGTAGATAAAGTAAAAGAGTGTGTGGATCATTTAAGTAGA GATGATGAGGACGAAGAGAAACTAGTAGCCTCATTATGGGGAGCAGAGAGATGTTTACGAGTTTTAGAGA GTGTAACAGTGCATAATCCAGAGAATCAAAGCTACTTGATAGCCTATAAAGATTCACAACTCATTATTTC ATCAGCTAAAGCATTACAGCATTGTGAAGACCTGATTCAGCAGTACAACCGTGCTGAGAACAGCATCTGT GTAGCAGACAGTAACCCTCTGCCTTACCAGAATGTAACTAACCATGTGGGCAAAGCAGTGGAGGACTGCA TGAGGGCTATAATTGGAGTATTGCTCAATTTAACTAATGATAATGAGTGGGGCAGCACAAAGACAGGAGA ACAAGAAGGACTCATAGGCACAGCGATGAACTGTGTTCTTCAGGTTCCAAAGTACCTACCTCAGGAGCAG AGATTTGATATTCGAGTGCTGGGATTGGGTCTACTCATAAACCTGGTGGAGTATAGTGCCCGGAATCGAC ACTGCCTTGTCAACATGCAAACATCCTGTTCCTTTGATTCCTCCTTCTCTAGTGGAGAAGGCGATCATAG TTTAAGGCTAGCCGGACAAGTTCATGCTGTTCAAGCTTTAGTGCAGCTATTTCTCGAACGAGAGAGAGCA GCACAATTGGCAGAAAGTAAAACAGATGAATTGATTAAAGATGCTCCTACCACTCAGCATGATAAGAGTG GAGAGTGGCAAGAAACAAGTGGAGAAATACAGTGGGTATCAACTGAAAAGACTGATGGTGCAGAGGAGAA GCAGAAGAAGGAGGAGGAGGATGAAGAACTTGACCTCAATAAAGCCCTTCAGCATGCTGGCAAACACATG GAGGATTGCATCGTAGCCTCCTACACAGCCCTGCTTCTTGGGTGTCTCTGCCAGGAAAGTCCAATCAATG TAACTACAGTAAGGGAATATCTTCCAGAAGGAGATTTCTCCATAATGACAGAGATGCTTAAAAAGTTCTT AAGCTTCATGAATCTTACGTGTGCTGTTGGAACAACAGGCCAGAAGTCTATCTCTAGAGTGATTGAATAT TTGGAACATTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301330
Insert Size	3585 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301330.1, NP_001288259.1
RefSeq Size	6353 bp
RefSeq ORF	3585 bp
Locus ID	218914
UniProt ID	Q65Z40
Gene Summary	Studies suggest that the protein encoded by this gene is important for the release of cohesin from chromatin. This gene product is thought to be essential for development, and reduced expression of this gene in cells causes defects in chromatin structure. High levels of expression of the human ortholog of this gene are observed in cervical cancers, and expression of the human ortholog of this gene in mice results in tumor formation. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Aug 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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