Cgn (NM_001293727) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330408J11Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229454 representing NM_001293727 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCTGATCCCCGGGGCCCCGTAGACCATGGAGTTCAAATTCGATTTATTACAGAGCCAGAGGGTG CTACTGAAATGGGCACCCTTCGTCGTAGTGGGCGGCGCCCTGCAAGGGATGCAAGAGCCAGTACCTATGG GGTCGCTGTGCGTGTGCAGGGCATCGCCGGGCAACCGTTTGTGGTTCTTAACAGTGGAGAGAAAGGGACT GACTCCTTTGGGGTCCAGATCAAAGGGGGCAACAACCGGGGGTCCCCAGGTGCACTGAGCTCCGATTCAG AGCTCCCCGAAAACCCCTACTCTCAAGTCAAGGGACGCCCTGCCACTTCTCGGAGCAGCACATCTGATGA GGAGCCCAAGGACCACTTGAATGGAAAACTGATCCGTTCCCAGTCGCAAGCCTCTCTGACCGGGCTTGCC TTTATGAGTCCTAGTAATAGGAGCACCAGCTTGCTGGAACTAGCCCCTAAACCGACTTCCTCGATTAACA CCATTGACACTGCCCCCTTGTCTTCAGTGGACTCGCTCATCAACAAGTTTGATAGTCAGAAGGGGGGGCA GGTCCGGGGTCGCACTGGGCGCCGCACAAGGACACTTCCCCACGAACAGCGCAAGCGGAGCCAGAGTCTG GACAGCAGGCTCCCACGGGATACACGTGAGGAACGGGAGCACCAGTCCGCTAACCACTGGACCCGCGGCA CAAAGTATGACAACCATGTGGACAGTTCAAAGAACCCATCGCAGAAGCAGAGCCCGTTCAGCAGCTTCAG CCGTTCGCGTCAGACTCAAGACTGGGTGCTTCAGAGTTTTGAGGAGACACGGGATCCAGCCATGGTGCAG TTCAAATCAACTCCAGACCTTCTCAGAGACCAGAGAGAGACAGCGCCACCAGGCAGTGCGGACCATGTGA AGGCCACCATCTATGGCATCCTAAGGGAGGGAAGCTCGGAGAGTGAGGCATCTGTGAGGAGGAAAGTTAG TTTGGTGCTGGAGCAAATGCAGCCTCTGGGGGCCCTGGCAGGACAGGCTGAGCTTACCCGAAAAATGGAG GAACTTCAGAAGAAGCTGGATGAGGAGGTGAAGAAGCGGCAGAAGCTAGAACCATCCAGGGTTGGGCTGG AGAGGCAGCTGGAGGAGAAGGCTGAAGAGTGCCACCGACTGCAGGAGTTGCTGGAGAGGAGAAAGGGGGA GGTCCAGCAGAGCAGCAAGGAACTTCAGAACATGAAGCTCCTGCTAGGCCAGGAAGAAGGACTACGGCAT GGGCTGGAGGCCCAGGTGAAGGAGCTACAGCTTAAGCTCAAGCATAGCCAGAGTCCCGATTCTGGAAAGG AAAGTCTCCTAAAGGACTTGTTAGACACCCGGGAACTTCTAGAAGAACTCTTAGAGGGCAAACAACGAGT AGAGGAGCAGCTCAGGCTGAGGGAACGGGAGCTGACAGCCCTGAAGGGGGCCCTGAAAGAGGAGGTGGCC TCCCACGACCAGGAGGTGGAACATGTCCGGCTGCAGTACCAGCGAGACACGGAGCAGCTTCGCCGGAGCA TGCAAGATGCGACCCAGGACCATGCTGCACTGGAGGCTGAGAGGCAGAAGATGTCTTCCCTCGTGCGGGA GCTGCAAAGAGAGCTAGAGGAGACCTCTGAGGAGACAGGGCATTGGCAGAGCATGTTCCAGAAGAACAAG GAAGAGCTGAGAGCCACCAAGCAGGAACTGCTGCAACTGCGGATGGAGAAGGAGGAGATGGAAGAGGAAC TTGGGGAGAAGATGGAGGTCTTACAGAGGGACTTAGAGCAGGCCCGAGCCAGTACAAGAGATACTCACCA GGTTGAGGAACTTAAAAAGGAGCTTCGACGCACCCAGGGGGAGCTTAAAGAGCTTCAGGCAGAGCAGCAG AACCAGGAGGTGACTGGGCGACACCAGAACCAGGTGTTAGAGAAGCAGCTGGCAGCGCTGAGGGAGGAGG CTGATCGAGGCCGAGAGCTGGAGCAGCAGAACCTCCAGCTACAAAAGACCCTGCAGCAACTGCGGCAGGA CTGTGAAGAGGCTTCCAAGGCTAAGGTGGCATCAGAGACAGAGGCCATGGTGCTGGGGCAGCGTCGGGCA ACCGTGGAAACCACACTTCGGGAGACACAAGAGGAAAATGACGAATTCCGAAGGCGCATCCTGGGTCTGG AGCAGCAGTTGAAGGAGGCCCGAGGTCTGGCGGAAGGTGGGGAAGCGGTGGAGGCACGGCTTCGGGACAA AGTGCATCGGCTGGAGGTAGAGAAACAGCAGCTGGAAGAGGCTTTGAATGCTGCCCGGGAGGAGGAGGGG AACCTGGCAGCAGCCAAGCGGGCTCTGGAGGTCCGGCTAGATGAGGCCCAGCGGGGGCTGGCACGCCTTG GGCAGGAACAGCAAGCACTGAACCGGGCCCTAGAGGAAGAGGGGAAGCAGCGGGAGGCTCTCCGGAGGAG CAAGGCAGAGTTGGAGGAGCAGAAGCGTTTGCTGAACAGGACTGTAGACCGACTCAACAAAGAGCTGGAG CAGATTGGGGATGACTCCAAGCTAGCCCTGCAGCAGCTCCAGGCTCAGATGGAAGATTACAAGGAAAAGG CCCGGAAGGAAGTAGCTGATGCCCAGCGTCAGGCCAAGGATTGGGCCAGTGAGGCTGAAAAGAATTCAGG GGGGCTGAGCCGGCTTCAGGATGAGCTGCAAAGGCTGCGGCAAGCCCTGCAGACATCCCAGGCTGAGCGG GACACAGCCAGGCTGGACAAAGAGCTGCTGGCCCAGCGACTGCAGGGTCTAGAGCAAGAGGCTGAGAACA AGAAACGCTTCCAGGACGACAAGGCTCGACAGCTGAAGAGCCTAGAGGAGAAAGTCTCGCGGCTGGAAGC GGAGTTAGATGAAGAAAAGAACACTGTGGAACTGTTAACAGATCGGGTGAACCGTGGCCGGGATCAGGTG GATCAACTGAGGACAGAGCTCATGCAGGAGAGATCTGCTCGACAGGACTTGGAGTGTGACAAAATCTCCT TGGAGAGACAGAACAAGGATTTGAAGACCCGGTTGGCCAGTTCAGAGGGCTTCCAGAAACCCAGCGCTAG CCTCTCCCAGCTTGAGTCCCAGAATCAGCTGCTGCAAGAGCGGCTGCAAGCTGAAGAGAGAGAGAAGACA GTTCTGCAGTCCACCAATCGCAAACTAGAACGGAGGGTTAAGGAGCTCTCCATCCAGATTGATGATGAAC GGCAGCATGTCAACGACCAGAAGGACCAGCTAACCCTGAGGGTGAAGGCTTTGAAGCGGCAGGTGGATGA AGCAGAAGAGGAAATTGAGCGGCTGGACAGCCTGAGGAAGAAGGCACAGCGGGAACTGGAGGAACAGCAT GAGGTCAATGAACAGCTCCAAGCCCGGATCAAATCCTTGGAAAAGGATGCCTGGCGGAAAGCTTCCCGCT CAGCTGCTGAGTCAGCCCTCAAACAAGAGGGGCTGAGCTCTGATGAGGAGTTTGACAACGTCTACGACCC CTCATCCATTGCATCACTGCTTACTGAGAGCAACCTGCAGACTAGCTCATGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293727
Insert Size	3555 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293727.1, NP_001280656.1
RefSeq Size	5015 bp
RefSeq ORF	3555 bp
Locus ID	70737

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