Prdm16 (NM_001291029) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730557K01Rik; csp1; mel1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229447 representing NM_001291029 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGATCCAAGGCGAGGGCGAGGAAGCTAGCCAAAAGTGACGGTGACGTTGTAAATAATATGTATGAAC CTGACCCGGACCTGCTGGCCGGCCAGAGTGCCGAGGAGGAGACCGAAGACGGCATCCTGTCCCCCATCCC CATGGGGCCACCGTCCCCCTTCCCCACCAGCGAGGACTTCACTCCCAAGGAGGGCTCGCCCTATGAGGCT CCTGTCTACATTCCTGAAGACATTCCAATCCCACCAGACTTCGAGCTACGAGAGTCCTCCATACCAGGAG CTGGCCTGGGGATCTGGGCCAAGCGGAAGATGGAAATCGGGGAGAGGTTTGGCCCCTACGTGGTGACGCC CCGGGCCGCACTGAAGGAGGCCGACTTTGGATGGGAGCAGATGCTGACGGATACAGAGGTGTCATCCCAG GAGAGCTGCATCAAAAAGCAGATCTCTGAAGACTTGGGTAGCGAGAAGTTCTGCGTGGATGCCAATCAGG CGGGGTCTGGCAGCTGGCTCAAGTACATCCGTGTAGCGTGTTCCTGTGATGACCAAAACCTCGCCATGTG TCAGATCAACGAACAGATTTACTATAAAGTCATTAAGGACATCGAGCCTGGAGAGGAACTGTTGGTGCAT GTGAAAGAAGGTGCCTACTCCTTGGGTGTCATGGCCCCCAGCTTGGATGAGGACCCCACATTCCGCTGTG ATGAGTGTGATGAGCTCTTCCAGTGCAGGCTGGACCTGAGGCGCCACAAGAAGTACGCGTGCAGCTCTGC AGGAGCCCAGCTCTACGAGGGCCTAGGGGAGGAACTCAAGCCCGAGGGCCTTGGCGTGGGCAGCGACGGG CAAGCGCATGAGTGCAAGGATTGCGAGCGGATGTTCCCCAACAAGTACAGCTTGGAGCAACACATGATCG TCCACACGGAAGAGCGTGAGTACAAATGTGACCAGTGTCCCAAGGCCTTCAACTGGAAGTCCAACCTCAT CCGCCACCAGATGTCTCACGACAGTGGCAAGCGCTTCGAATGTGAAAACTGTGTCAAGGTGTTCACGGAC CCCAGCAACCTCCAGCGTCACATCCGCTCACAGCATGTCGGTGCCCGGGCCCATGCCTGCCCTGACTGTG GCAAGACCTTCGCCACATCCTCTGGCCTCAAACAGCACAAGCATATCCACAGCACGGTGAAGCCATTCAT ATGCGAGGTCTGCCACAAGTCCTACACGCAGTTCTCCAACCTGTGCCGGCACAAGCGGATGCACGCCGAC TGCAGGACGCAGATCAAGTGCAAGGACTGTGGGCAGATGTTCAGCACTACCTCCTCCCTCAACAAGCATC GGAGATTCTGCGAGGGCAAGAACCATTACACGCCTGGCAGCATCTTCACCCCAGGCCTGCCCTTGACCCC CAGCCCCATGATGGACAAGACAAAACCCTCCCCGACCCTCAACCACGGGGGCCTAGGCTTCAGCGAGTAC TTCCCCTCCAGACCTCATCCTGGGAGCCTGCCCTTCTCGGCTGCTCCTCCGGCCTTCCCCGCACTCACTC CGGGCTTCCCGGGCATCTTTCCTCCATCCCTGTACCCACGACCACCTCTGCTACCTCCCACGCCGCTGCT CAAGAGCCCCCTGAACCACGCGCAGGACGCCAAGCTACCCAGCCCGCTGGGAAACCCAGCCCTGCCCCTT GTCTCCGCGGTCAGCAATAGCAGCCAGGGTGCCACAGCGGCCACCGGGTCAGAGGAGAAATTTGATGGCC GCTTGGAAGACGCATATGCGGAGAAGGTCAAAAATAGGAGCCCTGACATGTCGGATGGCAGTGACTTTGA GGATATCAACACCACGACCGGGACAGACTTGGACACTACCACGGGCACGGGGTCAGACCTGGACAGCGAC CTGGACAGTGACAGAGACAAAGGCAAGGACAAGGGGAAGCCAGTGGAGAGCAAACCTGAGTTTGGGGGTG CATCTGTGCCCCCTGGGGCCATGAACAGTGTGGCCGAGGTACCGGCCTTCTACTCACAGCATTCCTTCTT CCCGCCACCCGAGGAACAGCTGCTGACGGCCTCGGGAGCTGCCGGCGACTCCATCAAGGCCATCGCGTCC ATCGCGGAGAAATACTTCGGTCCTGGCTTCATGAGCATGCAGGAGAAGAAGCTGGGCTCACTACCCTACC ACTCCGTGTTCCCCTTCCAGTTCCTGCCTAACTTTCCCCACTCCCTCTACCCCTTTACGGACCGAGCCCT CGCCCACAACTTGCTGGTCAAGGCTGAGCCAAAGTCACCCCGGGATGCCCTCAAGGTGGGCGGCCCCAGT GCGGAGTGCCCCTTCGACCTCACCACCAAACCAAAAGAGGCCAAACCCGCCCTGCTCGCACCCAAGGTCC CCCTCATCCCCTCATCTGGCGAGGAACAGCCACTGGACCTGAGCATCGGCAGCAGGGCCAGGGCAAGCCA GAACGGAGGTGGCCGTGAGCCGCGGAAGAACCACGTCTACGGTGAACGGAAGCCGGGGGTCAGCGAGGGG CTGCCTAAGGTGTGCCCAGCACAGCTGCCCCAGCAGCCCTCCTTGCATTATGCTAAGCCTTCACCGTTCT TCATGGATCCCATCTACAGCAGGGTAGAAAAGCGGAAGGTGGCAGACCCTGTGGGAGTCCTGAAAGAGAA GTACCTGCGGCCGTCCCCACTTCTGTTCCACCCCCAGATGTCAGCCATAGAAACCATGACGGAGAAGCTG GAGAGCTTTGCAGCCATGAAGGCCGACTCAGGCAGCTCCCTGCAGCCCCTGCCTCACCACCCGTTCAACT TCCGCTCCCCACCCCCAACGCTCTCGGATCCCATCCTCAGGAAGGGGAAGGAGAGATACACGTGCAGGTA CTGTGGCAAGATCTTCCCCAGATCTGCAAATCTCACAAGACATCTGAGGACACACACAGGGGAGCAGCCA TACAGGTGCAAGTACTGTGACCGGTCATTCAGCATCTCCTCCAACCTCCAGCGGCACGTGAGGAACATCC ACAACAAAGAGAAGCCGTTCAAGTGCCATCTGTGCAACCGCTGCTTCGGGCAGCAGACCAACCTAGACCG GCACCTGAAGAAGCACGAACACGAGGGCGCACCAGTGAGCCAGCACTCCGGGGTGCTCACGAACCACCTG GGCACCAGCGCCTCCTCCCCCACCTCCGAGTCGGACAACCATGCACTTTTAGATGAGAAGGAAGATTCTT ACTTCTCCGAGATCCGAAACTTCATCGCCAACAGCGAGATGAACCAGGCATCCACTCGAATGGACAAACG GCCTGAGATCCAAGACCTGGACAGCAACCCACCGTGTCCAGGCTCAGCCAGTGCAAAGCCAGAGGACGTA GAGGAGGAGGAAGAGGAGGAGCTGGAGGAAGAGGATGATGACAGCTTAGCCGGGAAGTCACAGGAGGACA CGGTGTCCCCCACACCTGAGCCCCAAGGAGTCTATGAAGATGAAGAGGATGAGGAACCACCCAGCCTGAC CATGGGCTTTGACCATACCCGGAGGCATATGCAATGA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_001291029
Insert Size	3537 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291029.1, NP_001277958.1
RefSeq Size	8436 bp
RefSeq ORF	3537 bp
Locus ID	70673
UniProt ID	A2A935
Gene Summary	Binds DNA and functions as a transcriptional regulator (PubMed:18483224). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (PubMed:22939622). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (PubMed:22939622). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (PubMed:17618855, PubMed:18483224). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (PubMed:18719582, PubMed:19641492). Functions as a repressor of TGF-beta signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate in-frame splice site each in the 5' and 3' coding regions, and lacks an alternate exon in the 3' coding region, compared to variant 1. It encodes isoform 4, which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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