Anks1 (NM_001286041) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Anks1a; mKIAA0229
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229433 representing NM_001286041 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGAGCAGGAGCTGCTGGAGGCGGCCCGCACCGGGCACCTCCCGGCCGTGGAGAAGCTGCTGT CCGGGAAGCGGCTCTCCTCCGGCTTCGGCGGCGGCGGCGGCGGCTCAGGCAGCGGCGGCGGCTCCGGGGG CGGCGGCCTCGGTTCTTCTAGCCACCCGCTCTCCAGTCTGCTGAGCATGTGGAGAGGGCCGAACGTGAAC TGTGTAGATAGCACCGGCTACACGCCACTACACCACGCTGCTTTGAATGGACACAGGGATGTGGTCGAGG TCCTTCTGAGGAACGACGCGCTGACCAACGTTGCTGACTCGAAAGGCTGCTACCCGCTGCACTTGGCAGC GTGGAAAGGAGACGCCCAGATAGTACGTCTGCTCATCCAGCAAGGGCCCTCACACACCAGAGTCAACGAA CAGAACAACGACAATGAGACGGCCCTGCACTGCGCGGCCCAGTACGGCCACACGGAGGTCGTGAAGGCTC TGCTGGAGGAGCTCACGGACCCCACCATGCGCAACAACAAGTTTGAAACGCCCCTGGACTTGGCGGCGCT GTACGGGCGGCTGGAGGTGGTGAAGCTGCTGCTGGGTGCACACCCCAATCTCCTGAGCTGCAGCACTCGG AAACACACGCCCCTGCACTTGGCAGCAAGGAATGGCCACAAGGCCGTGGTGCAGGTCCTGCTGGACGCCG GCATGGATAGCAACTATCAGACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGA TGTGGTGCAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTT GACACTGTCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCG GAAAAAGAAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGC CATAGCACCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACAT GCTGACGAGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCA GTGGACGATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGT GAGGCCTAGGGAACGTCCGCCGCCTCCAGCAAAACCGCCACCCGATGAAGAGGAGGAAGAACGCGTAGAT AAGAAGTATTTTCCCTTGGCAGCCTCTGAGGGCCTGGCTGTGAGACCCAGGATTCAGAGTAGTGCTCCCC AGGAAGAGGAGGAGCACCCATATGAGCTGCTGCTCACGGCAGAAACCAAGAAGCTGGGGACCACAGACGG GAGGACTGAAGACCACAGGCAGAGCGGCAGCGGCCGGAGCCAGGACTCTGTGGAGGGGCAGGACGGGCAG GTCCCAGAGCAGTTCTCAGGTCTCCTCCACGGCTCCTCCCCAGTGTGTGAGGTGGGGCAGGACCCTTTCC AGCTGCTCACGGCCCCCAGCCAGAGCCACCCGGAGTCCTCCCAGCAGGACGCCTGCCACGAGGCCAGCAT GCAGCTAGAGGAGCCAGGCGTGCAAGGCACTGAACCCCCGCAGCCCGGCGTCCCTGACCAAAGCAAGAGA GTGGGCTTGCCGGCAGGCCTGACAGCCCTGGCCAGCAGAACATACCTCGATGCATTGACTCACACAGTAC CTCTGCGCCCTGCTGGTGCCGAGGAAGAAGACCAGAGCGGGCCCAGAAGCAGAGCCCCTCCCACCAGCAA GCCCAAAGCTGAGCTCAAACTCAGCCGCAGTTTGTCTAAGTCTGACTCTGACCTCCTGACCTGCTCCCCC ACGGAGGACGCCACAATGGGGAGTCGCAGCGAGTCCTTGTCCAACTGCAGCATCGGGAAGAAGAGGTTGG AGAAATCACCTTCCTTTGCCTCAGAGTGGGATGAGATTGAGAAAATCATGAGCTCCATTGGGGAAGGGAT TGACTTCTCTCAGGAGCAGCAGAAGATCTCAGGTTCTCGGACGCTGGAGCAGAGCGTCGGGGAGTGGCTG GAGTCGATTGGCCTGCAGCAGTACGAGAGCAAGCTGCTGCTGAACGGCTTTGATGATGTCCGCTTCCTGG GGTCTAATGTGATGGAAGAGCAGGACCTGAGGGAGATTGGCATCAGTGACCCACAGCACCGGCGGAAGCT GCTGCAGGCTGCACGCTCCTTGCCCAAGGTGAAGGCCCTTGGCTATGACGGGGTCAGCCCCACCTCAGTG CCCTCATGGCTGGACTCGCTGGGGCTGCAGGACTATGTCCACTCTTTCCTGTCCAGTGGCTACAGCTCCA TCGACACTGTGAAGAACCTCTGGGAGCTAGAGCTTGTCAATGTCCTGAAAGTTCACCTGCTGGGCCACCG CAAGCGCATTATCGCCTCTCTAGCAGACAGGCCCTATGAGGAGCCACCCCAGAAGCCTCCAAGGTTTTCC CAGCTAAGATGCCAGGATTTAATCTCCCAGACATCGTCCCCGCTGAGCCAGAATGATTCCTGCACAGGGC GGTCGGCAGACCTGCTGCTGCCCTCAGCAGACACAAGCAGGAGGCGGCACGACAGCCTGCCCGACCCTGG GACCGCCTCCCGAGCAGACCGCTTCCGAGTCCAGGAGGAGCCCAGTGAGACCAAGCTGACCCTCCGTCCA CCCAGCCTAGCCGCGCCCTATGCTCCTGTTCAGAGCTGGCAGCACCAGCCAGAAAAGCTCATCTTTGAGT CCTGCGGTTATGAGGCCAATTATCTGGGTTCCATGCTGATCAAAGATCTGCGAGGGACAGAGTCCACCCA AGATGCCTGTGCCAAAATGCGGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATC ACGTACAAAGGTGTCAAGTTCATCGATGCTTCCAACAAGAACGTCATTGCGGAGCACGAGATCCGGAACA TTTCCTGCGCCGCGCAGGACCCAGAGGACCTGTGTACCTTCGCGTACATCACCAAGGACCTGCAGACGAG CCACCACTACTGCCACGTTTTCAGCACAGTGGATGTGAACCTGACCTACGAGATCATCCTGACCCTGGGG CAGGCATTTGAGGTGGCCTATCAGCTGGCCCTCCAAGCCCAGAAATCCAGGACCATGGCGGCCTCTGCAG CTTCGATGATTGAGACAAAATCCTCCAAACCAGTGCCTAAGCCTCGGGTCGGCATGAGGAAGTCTGCAGT GCCGGTGCCCCCCGACTCTCGCTGTTGTCACTGTCACACCTGCACCACTCACCGTCCTTCCTACCTACCG CTGCCGTCCGTTAGTCCTGGAGTCAAGCTGGAACCACCTGATTCTGACCAAGAGGCCCCGTCCCACGCTA GTGTTTCCTGGATCGTGGACCCAAAGCCAGACTCGAAGCGGAGCCTCAGCACCAAGTACGAGACCACTAT CTTCTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001286041
Insert Size	3507 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286041.1, NP_001272970.1
RefSeq Size	7029 bp
RefSeq ORF	3507 bp
Locus ID	224650
UniProt ID	P59672
Gene Summary	Regulator of different signaling pathways. Regulates EPHA8 receptor tyrosine kinase signaling to control cell migration and neurite retraction.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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