Nol8 (NM_001271397) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921532D18Rik; 5730412B09Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229429 representing NM_001271397 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGACCTCTGGAAAACGTTTCCAGCAATCTTGATAGATGCCCAACATCCATGCAAGGCAACAGAG AAATGAAGCGCCTCTTTGTGGGTGGACTTGGACAGGGCATTTCTGAGACAGACCTACAAAATCAGTTCGG CAGATTTGGAGAAGTTTCTGACGTGGAGATCATCACTCGGAAAGATGACCAAGGAAATTCACAGAAAGTC TTTGCGTATGTTAACATTCAAATAACAGAAGCGGACCTGAAGAAATGTATGTCTATTTTAAACAAAACAA AATGGAAAGGTGGAACACTACAAATTCAGCTAGCAAAAGAAAGCTTTTTACACAGATTGGCCCAAGAAAG AGAAGACGCAAAAGCAAAGAAAGAAAAATCAACAACAGGCAACCCCACCTTATTGGAAAAGATGGGAGCA GTGGATTTCCATATGAAGGCTGTGCCTGGAACAGAAGTACCAGGGCATAAAAATTGGGTTGTGAGTAAGT TTGGAAGAGTCTTACCTGTTCTTCACCTTAAGAATCAACAAAAACATAAAATTATGAAATATGATCCATC AAAATACTGCCACAATATAAAAAAGATTCCAGAGAATTTGACAGAAACCACTCCCATAGCTGAACTCACT TGGGAATTGGAAGGAGGCAATGACCCTATGAGTAAGAAACGTCGAGGAGAGTTCTCTGACTTTCATATCC CTCCTCAAAAGGTCAAGAAAGTGCAGAAGAGCAATGATCCCATGGAGTCCAAGGTTTCTAATATTGGTCT AAGGACTAATCAGGTAATGGAGAAGAACAAATCAACACATCCTGTGACTGCACACGGGACAGCCCCTAGT ACTGTTAATCCTTCTAAACAACTCCTTGTGTCCAGTTCTGGTACTCAGAAACCTAAACATGTGGTTTTTC ATAATTCTGACTTTGAAATTATCTGGAATAAAAGTAGCATGTCTGATGATGATGTTGACTCGGAAGATGA ATTAAAAATGATGATTGCAAAAGAGGAAAACAGAGAGAAACCTGGGCATTCCTCAGTCAATGAGTCTGAA CATGATACTTTTGAAGTTGTTAGGGATGATTTCAAATCAAATATTCACAAGCTTTCTTCTTCAGTAAGCC TAGGAAATAACCATGAATATGATTCAAGTGATACAGATGAAATTATTGCAATGAAAACAAAAAATGCTAA GGTCAAAAACAGTGCAGAATCTTCACAACCAGAAAGGACTGTAAGCAAGAAAAGTTCTTTTCAAAAGATA GAACCTTCTAATGACTGTATAAAAGTACAAGGAATTAACAGCAACAAAGAATCAGCCCTGTGTCATGGAG TAAAGTTTGTAAATCCCAAATTTCCACCTGACTCCAGTGGCAGTGACAGTGAAGAGTCCGAAGAAGATGA AGAATATAAAGTCTTGATGGAAAACTGTCCCCGTGTGAGTCTCACTTTGGCTGACTTGGAGCAGTTGGCT GGCAGTCATCGGAAGTTTCCAGGAAAAGACAGTGAGACTAATGGTCCACAAAATGACAGTCACTGCAAAT TTGACACAACCTCCAAGAATCCCAAGACTTCTGGTGACCTGTACAATGGCAGACAACAGTGCATTCTTCC TGAGGAGATTGTGGCCTCCCTTTTAGAGGACGAGAATACTTACAGCAAACAAAAATCGGAAGAAGATATC TTAAAGCCAAAATTCCAGGCCTTCAAGGGAATAGGCTGTCTCTATGCAAAGGAGTCAGTAGACAAAACTT TGAAAGAGAATATTGCCTTTAATACTGGCGGTGGGCATCACAGTTCCTTAAAACATGAAGATCACAATAG AAGTTTGATGGAAAATGGATCTAAGTGTGTTAATGGCTCATCAAGCAAACTGACTTCATGCCAACCTGCA AAGAAGGTGAATGACCCGAACCACATTCAGCCTCCAAAAAGACAGTGTACTTTTGAGAACCAGAACCATA AAGTAATGTCCTCTACTAGTTGTGACAAGGGAAGTACAAATCCTCTCCCAGGTCCGTTGCCATTGAAAGC TAAAACATCCCTGCATCTTAGTGCCAACAGTCACAAGGTAGACTCTGATGGAGACGCTTGCCATTGGCCT GAAAGTAGAAAAGCTTTAGAGAAAGAGAGGACCAATTTAAGCAACCTCGAATCTCTTGAGAAATCATCAA AGGTGTCTCCCAGGGAAGACCCTCAGAAAAGCCCAGCTGGTTTCTCACTTTCTGATAGTAATGCATCCTG TATAAATGCTAAGGATAAACAAGCTGAAGACAACCAGAAGCGATTGGCCGCCTTGGCAGCATGGCAGAAA GCCAGGGAAGTACAGAAAAAACTGGTGCATAGTGCCCTGGCAAATTTGGATGGTCATCCAGAGGACAAAA AAACACACATTGTCTTTGCTTCTGATAATGAAAGTGAAACGGAAGAGACATCCACTCAGGAGCAAAGCTG TCCAGAAAAGGAGCTGATGAAAGAATCTGTGAGTAAATCGCCTGGGAAGCTGTTTGACAGCAGTGATGAT GAAGACTCAGATTCCAAGGAGGACAGCACCAGGTTCAGCATTAAACCTCAGTTTGAGGGCAGAGCTGGCC AGAAGCTTATGGATCTGCAGTCTCAGTTTGGAAGTGATGAGAGATTCCGCATGGACTCTAGATTTTTAGA GAGTGACAGTGAGGACGAAAAGAAAGAGCTAAATGAAGATAAAGTGAATGAAGACGAGCTTGCTGCAGAA AAAAAGAAAACCCTGAATGTTGTGCAAAGTGTTTTGAACATCAATGTGAACAACCCGACGAACAAAGGAT CAGTGGCTGCTAAGAAATTTAAGGATATCGTACATTATGACCCAACAAAGCATGACCATGCCATTTATGA AAGAAAACAAGAAGATAAAGAAAAAGAAAGTAAGGCAACACGGAAGAAGAAAAAGGAAGAAGCAGAGAAG CTGCCTGAGGTATCCCAAGACATGTATTATAACATTGCTGCAGATTTGAAAGAAATATTCCAAAGTATGA GCAACACAGATGAAAAGGAAGAAGATGTGCCCAGGACTGAGGCTGGTGCTAGAGAGGGGACTGGAAAAAT CAGGAACGCCGAGACACTGGCATGTGAGCCCGAGCAAACCACTGGCTTTACATTCTCTTTTTTTGATTCA GCCACTAAAGATGAAAAGGATGCCACCTATAGAATCGAACTAGTGAAACATGGGAAGATAGTCTGTCCAA ATGATCCCCGATTCCAAGACAGTAGTTCAGAAGAAGAAGATATTGCTGAAGAAGCAGATCATAGCAAGCC AAGCCCTGGAGAAGCGGTTCCTGAGAATGAGGCCATTAGATTTTTCTTTTTCTCTGAGAATGACGACAGA CTTCGTGGTTCTAACTTATTCTGGAGTGGTATGGGAGGTAGTATTAGCAGAAATTCTTGGGAAGCCCGAA CAAGTAGTCTCCTTTTGGAATGTCGGAAGAAACATAAAGAAGCCAAAAGGAAGGTGAAAGCAAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271397
Insert Size	3498 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271397.1, NP_001258326.1
RefSeq Size	4655 bp
RefSeq ORF	3498 bp
Locus ID	70930
UniProt ID	Q3UHX0
Gene Summary	Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the protein.

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