Itgal (NM_001253872) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	(p180); Cd11a; LFA-1; LFA-1A; Ly-15; Ly-21
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229427 representing NM_001253872 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTTCCGGATTGCGGGCCCCAGACTTTTGCTACTGGGACTCCAGCTGTTTGCCAAGGCCTGGAGCT ACAACCTGGACACACGGCCTACGCAGAGCTTCTTGGCACAAGCTGGAAGACATTTTGGGTACCAGGTCTT GCAGATTGAAGATGGGGTTGTCGTGGGAGCCCCAGGTGAGGGGGACAACACGGGAGGCCTCTATCACTGC CGAACAAGCAGCGAGTTCTGCCAGCCAGTCAGCCTACATGGTTCTAACCATACCTCCAAGTACTTGGGAA TGACGCTGGCAACAGATGCCGCCAAGGGAAGCCTTTTGGCCTGTGACCCTGGACTGTCTCGGACATGCGA TCAGAACACTTACCTCAGTGGCCTCTGCTACCTCTTCCCCCAGAGTCTGGAGGGACCTATGTTACAAAAT CGTCCCGCCTATCAGGAATGTATGAAGGGCAAAGTCGACCTGGTGTTTCTGTTCGATGGCTCACAGAGCT TGGATAGAAAGGACTTTGAAAAAATCCTGGAATTCATGAAGGATGTGATGAGGAAGCTCAGCAATACTTC CTACCAGTTTGCTGCCGTCCAGTTCTCCACAGACTGCAGAACAGAATTTACTTTCTTGGACTACGTTAAG CAGAACAAGAACCCCGATGTTCTGCTAGGCAGCGTGCAGCCCATGTTCTTGCTGACCAATACCTTTCGTG CCATCAACTATGTGGTGGCACACGTGTTCAAAGAAGAGTCTGGTGCCAGGCCGGATGCTACCAAGGTGCT TGTCATCATTACAGACGGGGAGGCAAGTGATAAAGGCAACATCAGTGCGGCCCACGACATAACCCGCTAC ATCATCGGGATTGGCAAGCATTTTGTGAGCGTACAAAAGCAAAAGACGCTCCACATATTTGCCTCAGAAC CTGTAGAGGAATTTGTGAAGATTCTGGACACCTTTGAGAAGCTGAAGGATCTTTTTACTGACCTGCAGAG GAGGATTTATGCTATTGAGGGCACAAACAGACAGGACCTGACATCCTTTAACATGGAACTCTCCTCCAGC GGGATCAGCGCAGACCTCAGCAAGGGCCATGCAGTTGTGGGAGCTGTTGGGGCTAAGGATTGGGCCGGGG GCTTTCTGGACCTGCGTGAAGACCTGCAGGGTGCCACATTTGTTGGGCAGGAACCGCTGACCTCAGATGT GAGAGGGGGCTACCTGGGTTACACTGTGGCCTGGATGACCTCCCGGAGCTCCAGACCCCTGCTGGCAGCA GGAGCCCCACGGTACCAGCATGTGGGACAAGTACTGCTTTTCCAAGCCCCAGAGGCTGGAGGACGTTGGA ACCAAACCCAGAAGATAGAAGGGACTCAGATCGGATCTTACTTTGGTGGGGAACTATGTAGTGTTGACCT GGACCAAGATGGCGAGGCAGAGCTGCTGCTGATTGGAGCACCCCTGTTCTTTGGGGAGCAGAGAGGAGGC CGAGTGTTCACTTACCAGAGAAGACAGTCGCTGTTTGAAATGGTCTCAGAGCTACAGGGTGACCCTGGCT ACCCGCTTGGTCGGTTTGGAGCCGCCATAACTGCCCTGACGGACATCAATGGGGATAGGCTGACTGATGT GGCTGTGGGAGCCCCTTTGGAGGAGCAGGGGGCTGTGTACATCTTCAATGGGAAGCCTGGTGGGCTCAGT CCCCAGCCAAGCCAGCGTATACAAGGAGCCCAGGTGTTCCCAGGAATCCGGTGGTTTGGCCGCTCCATCC ATGGGGTGAAGGACCTTGGAGGGGACAGGCTGGCAGATGTGGTTGTAGGAGCTGAGGGTCGGGTGGTTGT GCTGAGCTCCAGGCCGGTGGTGGATGTGGTCACTGAGCTGTCGTTCTCCCCAGAGGAAATCCCAGTGCAC GAGGTGGAGTGCTCCTACTCAGCCAGGGAGGAGCAGAAACACGGAGTCAAGCTCAAGGCATGCTTCCGGA TCAAGCCCCTCACGCCACAGTTTCAAGGTCGCCTGCTTGCCAACCTCAGCTACACCCTGCAGCTGGATGG CCATCGGATGAGGAGCCGAGGGTTGTTCCCAGATGGAAGCCACGAGCTCAGTGGAAACACCTCCATCACC CCAGATAAATCCTGCTTGGACTTCCACTTCCACTTCCCGATCTGCATTCAAGACCTCATCTCCCCTATCA ATGTCTCCCTGAATTTCTCTCTTTTGGAGGAAGAAGGAACACCAAGGGACCAAAAGGTGGGCAGGGCCAT GCAGCCTATCCTGAGACCTTCAATCCACACAGTGACTAAGGAGATCCCTTTTGAGAAGAACTGTGGTGAA GATAAGAAGTGTGAGGCAAACCTGACCCTGTCATCCCCTGCCAGATCTGGACCCCTGCGTCTGATGTCCT CTGCCAGCCTTGCTGTGGAGTGGACACTGAGCAACTCAGGGGAAGATGCCTACTGGGTGCGATTAGACCT GGACTTCCCTCGGGGACTCTCCTTCCGGAAAGTGGAGATGCTTCAGCCACACAGCCGAATGCCTGTGAGC TGCGAGGAGCTCACCGAGGGGTCAAGTCTCCTGACTAAGACACTGAAATGCAATGTAAGCTCTCCCATCT TCAAAGCAGGCCAGGAGGTGAGCCTCCAGGTGATGTTTAACACGCTACTCAACAGCTCCTGGGAAGACTT CGTTGAGCTGAATGGCACTGTGCACTGTGAGAATGAGAACTCAAGCCTCCAGGAGGACAACTCAGCCGCC ACCCACATTCCTGTCCTGTACCCTGTCAACATCCTTACTAAGGAGCAGGAGAACTCCACTCTCTATATCA GTTTCACCCCTAAAGGTCCCAAGACCCAACAAGTCCAGCATGTCTACCAGGTGAGGATTCAGCCATCTGC CTATGACCACAACATGCCCACACTAGAGGCCTTGGTTGGGGTGCCCTGGCCTCACAGTGAGGACCCCATC ACATACACATGGAGTGTACAGACGGATCCCCTTGTCACTTGCCACAGCGAGGACCTGAAGAGGCCGTCCA GCGAAGCTGAGCAGCCTTGTCTGCCTGGAGTCCAGTTCCGCTGTCCAATTGTCTTCAGGCGGGAGATCCT CATCCAAGTGACGGGGACCGTGGAACTCTCCAAGGAAATCAAGGCCTCCTCCACACTCAGCCTCTGCAGC TCACTCTCCGTCTCCTTCAACAGCAGCAAGCATTTCCATTTGTATGGCAGCAAAGCCTCCGAGGCCCAGG TCCTCGTGAAGGTTGACCTGATCCACGAGAAGGAGATGCTTCACGTGTACGTACTCAGCGGCATTGGGGG CCTCGTGCTTCTGTTCCTGATTTTCCTGGCGCTCTACAAGGTTGGCTTCTTCAAACGGAACCTGAAGGAG AAGATGGAGGCTGATGGAGGTGTTCCAAATGGAAGCCCTCCAGAAGACACTGACCCTCTGGCAGTACCTG GGGAAGAGACCAAAGATATGGGCTGTCTAGAGCCCCTCCGGGAGAGTGACAAGGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253872
Insert Size	3489 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253872.1, NP_001240801.1
RefSeq Size	5225 bp
RefSeq ORF	3489 bp
Locus ID	16408

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