Plcb2 (NM_001290790) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI550384; B230205M18Rik; B230399N12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229422 representing NM_001290790 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTGCTCAACCCTGTTCTATTGCCCCCTAACGTGAAGGCATATCTGAGCCAAGGGGAGCGCTTCA TCAAGTGGGACGATGAAACTTCAATAGCCTCTCCTGTTATCCTCCGTGTGGATCCCAAGGGCTATTACTT ATACTGGACATATCAAAATCAGAGCCAGAAACTCCGGGAGGTCTTCAACATGGACTTTCCAGACAACCAC TTCCTGCTGAAAACACTCACAGTGGTGTCCGGCCCTGACATGGTGGACCTCACCTTCTACAACTTTGTCT CTTACAAGGAGAACGTAGGCAAGGACTGGGCTGAGGACGTGCTAGCTCTCGCCAAACACCCGATGACAGT CAATGCTCCTCGAAGCACATTCCTGGACAAGATCCTGGTGAAGCTTAAGATGCAGCTCAACCCTGAAGGG AAGATTCCCGTGAAGAATTTTTTCCAGATGTTTCCTGCTGATCGAAAACGGGTGGAAGCTGCCCTCGGTG CTTGTCACCTTGCAAAAGGCAAAAATGATGCTATCAACCCAGAGGACTTCCCAGAATCCGTGTACAAGAG CTTCCTCATGAGCCTCTGTCCTCGGCCAGAAATCGACGAGATCTTCACTTCCTACCACTCTAAAGCTAAG CCCTACATGACCAAGGAACACCTGACCAAATTCATCAATCAGAAGCAGCGAGACCCTCGACTCAACTCCT TGCTGTTCCCACCAGCCCGGCCTGAGCAAGTTCAAGTGCTCATTGACAAGTACGAACCCAGCGGCATCAA TGTGCAGAGGGGCCAGCTGTCACCAGAGGGGATGGTCTGGTTTCTCTGTGGACCAGAGAACAGTGTGCTG GCCCACGATACACTGCTGATCCACCAAGACATGACACAACCACTGAATCACTATTTCATCAACTCCTCAC ACAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTTTCCTCGGCTGAGATGTACCGCCAGGTGCTGCT GTCTGGCTGCCGGTGTGTAGAGTTAGACTGTTGGAAGGGCAAGCCCCCTGACGAAGAGCCCATTATCACC CACGGCTTCACCATGACCACAGATATCTTGTTCAAGGAAGCAATTGAAGCCATTGCAGAAAGTGCCTTTA AGACCTCCCCGTATCCTGTCATCCTATCATTTGAAAACCATGTAGACTCACCCCGGCAACAGGCTAAGAT GGCTGAGTACTGCAGGAGCATGTTTGGAGAGACCTTGCTCACAGATCCCCTGGAAAATTTCCCTCTGAAA CCTGGCATCCCTCTGCCCAGCCCCGAGGACCTCCGGGGCAAGATCCTCATTAAGAATAAGAAGAACCAGT TTTCTGGGCCAGCATCCCCCAGCAAGAAGCCTGGTGGGGTGGCTGAGGGCAGCCTCCCGTCTAGTGTCCC TGTAGAAGAGGACACGGGGTGGACTGCTGAGGACCGGACTGAGGTGGAGGAGGAGGAGGTGGTGGAAGAG GAGGAAGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAAATTAAGAAGATGCAGTCGGATGAGGGCACAG CAGGCCTGGAGGTGACAGCTTATGAGGAAATGTCCAGCCTTGTCAATTATATCCAGCCCACCAAGTTTAT CTCCTTCGAGTTCTCTGCACAGAAGAACAGAAGTTACGTTGTCTCTTCCTTCACGGAGCTCAAGGCCTAC GAGCTGCTCTCCAAGGCCTCCATGCAGTTTGTGGACTATAATAAACGCCAGATGAGCCGTGTGTACCCCA AGGGCACACGCATGGACTCTTCCAACTACATGCCCCAGATGTTCTGGAATGCTGGATGTCAGATGGTTGC CCTCAATTTCCAGACAATGGACCTGCCCATGCAGCAGAACATGGCACTGTTTGAGTTCAACGGGCAGAGT GGCTACCTCCTAAAGCACGAGTTCATGCGCAGACTGGACAAGCAGTTCAACCCCTTCTCAGTGGACCGCA TTGATGTGGTGGTAGCCACCACCCTTTCCATTACGATCATCTCTGGGCAGTTCCTGTCAGAGCGCAGTGT ACGCACCTATGTGGAAGTGGAACTGTTTGGCCTCCCAGGGGACCCCAAGAGGCGGTATCGAACCAAGCTG TCACCTACTGCTAACTCCATCAATCCTGTCTGGAAAGAGGAACCCTTTATCTTTGAGAAGATCTTGATGC CTGAGCTGGCCTCCCTCAGGATAGCTGTGATGGAAGAAGGCAGCAAATTTCTTGGACACCGCATCATCCC CATCAATGCCTTACATTCTGGATACCACCATCTGTGCCTGCGCAGTGAGAGCAACATGGCCCTTACCATG CCAGCTCTCTTTGTCTTCCTGGAGATGAAGGACTACATACCTGACACCTGGGCAGATCTTACAGTAGCCC TTGCCAACCCCATCAAGTACTTCAATGCCCAGGATAAGAAGTCCGTGAAGCTCAAAGGAGTGACAGGAAG TCTGCCCGAGAAACTCTTCTCTGGGACACCTGTTGCCAGTCAGTCCAATGGGGCGCCAGTCTCGGCAGGC AATGGGTCAACAGCGCCTGGGACCAAGGCCACGGGGGAAGAAGCTACGAAAGAAGTGACAGAGCCACAGA CCGCCAGCTTGGAAGAGTTGCGGGAACTGAAGGGCGTGGTGAAACTGCAGCGAAGACATGAGAAGGAGCT TCGAGAGTTGGAGCGCCGTGGAGCCCGGCGCTGGGAGGAACTGCTGCAGCGTGGCGCCGCACAGCTGGCG GAGCTCCAGACCCAGGCAGCAGGCTGCAAGCTCCGCCCAGGCAAGGGCTCCCGCAAGAAGAGGACCCTGC CCTGTGAGGAGACCGTCGTGGCGCCTAGCGAGCCCCACGACCGGGCGGACCCGCGCGTGCAGGAGCTGAA GGACAGGCTGGAGCAGGAGCTGCAGCAGCAAGGCGAGGAGCAGTACCGCTCCGTCCTCAAGCGCAAGGAG CAGCACGTGACCGAGCAAATCGCCAAGATGATGGAGCTGGCCAGAGAGAAACAGGCTGCTGAACTCAAGA CCTTCAAGGAGACCTCAGAAACTGACACCAAAGAAATGAAGAAAAAACTGGAGGCCAAGAGGCTGGAACG GATCCAAGCCATGACCAAGGTCACCACAGACAAGGTGGCCCAGGAGAGGCTCAAGAGAGAAATTAACAAC TCCCACATCCAGGAAGTGGTCCAGGCTGTCAAGCAGATGACAGAGACCCTGGAGCGTCACCAGGAGAAGC TGGAAGAGAGGCAGACAGCCTGCCTGGAGCAGATCCAGGCAATGGAAAAGCAGTTCCAGGAGAAGGCGCT GGCAGAGTATGAGGCCAAGATGAAGGGCCTGGAGGCTGAAGTAAAGGAGTCGGTGCGGGCCTACTTCAAG GACTGCTTCCCCACCGAAGCAGAAGACAAGCCTGAGAGGTCCTGTGAGGCCTCTGAGGAGTCGTGTCCAC AGGAACCACTTGTGAGCAAGGCAGACACTCAGGAGAGCCGCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290790
Insert Size	3477 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290790.1, NP_001277719.1
RefSeq Size	5091 bp
RefSeq ORF	3477 bp
Locus ID	18796
UniProt ID	A3KGF7
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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