Plcb2 (NM_001290790) Mouse Untagged Clone

CAT#: MC229422

Plcb2 (untagged) - Mouse phospholipase C, beta 2 (Plcb2), transcript variant 2



  "NM_001290790" in other vectors (1)

CNY 11,120.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI550384; B230205M18Rik; B230399N12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229422 representing NM_001290790
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTTGCTCAACCCTGTTCTATTGCCCCCTAACGTGAAGGCATATCTGAGCCAAGGGGAGCGCTTCA
TCAAGTGGGACGATGAAACTTCAATAGCCTCTCCTGTTATCCTCCGTGTGGATCCCAAGGGCTATTACTT
ATACTGGACATATCAAAATCAGAGCCAGAAACTCCGGGAGGTCTTCAACATGGACTTTCCAGACAACCAC
TTCCTGCTGAAAACACTCACAGTGGTGTCCGGCCCTGACATGGTGGACCTCACCTTCTACAACTTTGTCT
CTTACAAGGAGAACGTAGGCAAGGACTGGGCTGAGGACGTGCTAGCTCTCGCCAAACACCCGATGACAGT
CAATGCTCCTCGAAGCACATTCCTGGACAAGATCCTGGTGAAGCTTAAGATGCAGCTCAACCCTGAAGGG
AAGATTCCCGTGAAGAATTTTTTCCAGATGTTTCCTGCTGATCGAAAACGGGTGGAAGCTGCCCTCGGTG
CTTGTCACCTTGCAAAAGGCAAAAATGATGCTATCAACCCAGAGGACTTCCCAGAATCCGTGTACAAGAG
CTTCCTCATGAGCCTCTGTCCTCGGCCAGAAATCGACGAGATCTTCACTTCCTACCACTCTAAAGCTAAG
CCCTACATGACCAAGGAACACCTGACCAAATTCATCAATCAGAAGCAGCGAGACCCTCGACTCAACTCCT
TGCTGTTCCCACCAGCCCGGCCTGAGCAAGTTCAAGTGCTCATTGACAAGTACGAACCCAGCGGCATCAA
TGTGCAGAGGGGCCAGCTGTCACCAGAGGGGATGGTCTGGTTTCTCTGTGGACCAGAGAACAGTGTGCTG
GCCCACGATACACTGCTGATCCACCAAGACATGACACAACCACTGAATCACTATTTCATCAACTCCTCAC
ACAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTTTCCTCGGCTGAGATGTACCGCCAGGTGCTGCT
GTCTGGCTGCCGGTGTGTAGAGTTAGACTGTTGGAAGGGCAAGCCCCCTGACGAAGAGCCCATTATCACC
CACGGCTTCACCATGACCACAGATATCTTGTTCAAGGAAGCAATTGAAGCCATTGCAGAAAGTGCCTTTA
AGACCTCCCCGTATCCTGTCATCCTATCATTTGAAAACCATGTAGACTCACCCCGGCAACAGGCTAAGAT
GGCTGAGTACTGCAGGAGCATGTTTGGAGAGACCTTGCTCACAGATCCCCTGGAAAATTTCCCTCTGAAA
CCTGGCATCCCTCTGCCCAGCCCCGAGGACCTCCGGGGCAAGATCCTCATTAAGAATAAGAAGAACCAGT
TTTCTGGGCCAGCATCCCCCAGCAAGAAGCCTGGTGGGGTGGCTGAGGGCAGCCTCCCGTCTAGTGTCCC
TGTAGAAGAGGACACGGGGTGGACTGCTGAGGACCGGACTGAGGTGGAGGAGGAGGAGGTGGTGGAAGAG
GAGGAAGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAAATTAAGAAGATGCAGTCGGATGAGGGCACAG
CAGGCCTGGAGGTGACAGCTTATGAGGAAATGTCCAGCCTTGTCAATTATATCCAGCCCACCAAGTTTAT
CTCCTTCGAGTTCTCTGCACAGAAGAACAGAAGTTACGTTGTCTCTTCCTTCACGGAGCTCAAGGCCTAC
GAGCTGCTCTCCAAGGCCTCCATGCAGTTTGTGGACTATAATAAACGCCAGATGAGCCGTGTGTACCCCA
AGGGCACACGCATGGACTCTTCCAACTACATGCCCCAGATGTTCTGGAATGCTGGATGTCAGATGGTTGC
CCTCAATTTCCAGACAATGGACCTGCCCATGCAGCAGAACATGGCACTGTTTGAGTTCAACGGGCAGAGT
GGCTACCTCCTAAAGCACGAGTTCATGCGCAGACTGGACAAGCAGTTCAACCCCTTCTCAGTGGACCGCA
TTGATGTGGTGGTAGCCACCACCCTTTCCATTACGATCATCTCTGGGCAGTTCCTGTCAGAGCGCAGTGT
ACGCACCTATGTGGAAGTGGAACTGTTTGGCCTCCCAGGGGACCCCAAGAGGCGGTATCGAACCAAGCTG
TCACCTACTGCTAACTCCATCAATCCTGTCTGGAAAGAGGAACCCTTTATCTTTGAGAAGATCTTGATGC
CTGAGCTGGCCTCCCTCAGGATAGCTGTGATGGAAGAAGGCAGCAAATTTCTTGGACACCGCATCATCCC
CATCAATGCCTTACATTCTGGATACCACCATCTGTGCCTGCGCAGTGAGAGCAACATGGCCCTTACCATG
CCAGCTCTCTTTGTCTTCCTGGAGATGAAGGACTACATACCTGACACCTGGGCAGATCTTACAGTAGCCC
TTGCCAACCCCATCAAGTACTTCAATGCCCAGGATAAGAAGTCCGTGAAGCTCAAAGGAGTGACAGGAAG
TCTGCCCGAGAAACTCTTCTCTGGGACACCTGTTGCCAGTCAGTCCAATGGGGCGCCAGTCTCGGCAGGC
AATGGGTCAACAGCGCCTGGGACCAAGGCCACGGGGGAAGAAGCTACGAAAGAAGTGACAGAGCCACAGA
CCGCCAGCTTGGAAGAGTTGCGGGAACTGAAGGGCGTGGTGAAACTGCAGCGAAGACATGAGAAGGAGCT
TCGAGAGTTGGAGCGCCGTGGAGCCCGGCGCTGGGAGGAACTGCTGCAGCGTGGCGCCGCACAGCTGGCG
GAGCTCCAGACCCAGGCAGCAGGCTGCAAGCTCCGCCCAGGCAAGGGCTCCCGCAAGAAGAGGACCCTGC
CCTGTGAGGAGACCGTCGTGGCGCCTAGCGAGCCCCACGACCGGGCGGACCCGCGCGTGCAGGAGCTGAA
GGACAGGCTGGAGCAGGAGCTGCAGCAGCAAGGCGAGGAGCAGTACCGCTCCGTCCTCAAGCGCAAGGAG
CAGCACGTGACCGAGCAAATCGCCAAGATGATGGAGCTGGCCAGAGAGAAACAGGCTGCTGAACTCAAGA
CCTTCAAGGAGACCTCAGAAACTGACACCAAAGAAATGAAGAAAAAACTGGAGGCCAAGAGGCTGGAACG
GATCCAAGCCATGACCAAGGTCACCACAGACAAGGTGGCCCAGGAGAGGCTCAAGAGAGAAATTAACAAC
TCCCACATCCAGGAAGTGGTCCAGGCTGTCAAGCAGATGACAGAGACCCTGGAGCGTCACCAGGAGAAGC
TGGAAGAGAGGCAGACAGCCTGCCTGGAGCAGATCCAGGCAATGGAAAAGCAGTTCCAGGAGAAGGCGCT
GGCAGAGTATGAGGCCAAGATGAAGGGCCTGGAGGCTGAAGTAAAGGAGTCGGTGCGGGCCTACTTCAAG
GACTGCTTCCCCACCGAAGCAGAAGACAAGCCTGAGAGGTCCTGTGAGGCCTCTGAGGAGTCGTGTCCAC
AGGAACCACTTGTGAGCAAGGCAGACACTCAGGAGAGCCGCCTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001290790
Insert Size 3477 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001290790.1, NP_001277719.1
RefSeq Size 5091 bp
RefSeq ORF 3477 bp
Locus ID 18796
UniProt ID A3KGF7
Gene Summary The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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