Hdac9 (NM_001271386) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AV022454; D030072B18Rik; HD7B; HD9; Hdac7b; HDRP; Mitr; mKIAA0744
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229355 representing NM_001271386 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCGCCGGTGCAGCCAGATGGAGTGGCCGGACGGGAGCAGCTCTTGGCTCAGCAAAGAATGCACA GTATGATCAGCTCAGTGGACGTGAAATCAGAGGTTCCTATGGGCCTGGAGCCCATCTCACCTTTAGACCT GAGGACAGATCTCAGGATGATGATGCCTGTGGTGGATCCTGTCGTCCGCGAGAAGCAACTGCAGCAAGAG TTACTTCTTATTCAGCAGCAGCAGCAGATCCAGAAGCAGCTCCTCATAGCAGAGTTTCAGAAGCAGCACG AGAATTTGACAAGGCAGCATCAAGCACAACTCCAGGAGCACATCAAGTTACAACAGGAACTTCTAGCCAT AAAACAACAGCAAGAACTCCTAGAAAAGGAGCAGAAACTGGAGCAGCAGAGGCAAGAACAGGAAGTAGAG AGGCATCGCAGAGAGCAGCAGCTTCCTCCTCTCAGAGGCAAAGATAGAGGACGAGAAAGGGCAGTGGCAA GCACAGAGGTTAAGCAGAAGCTTCAAGAATTCCTGTTGAGTAAATCAGCAACAAAAGACACTCCAACCAA TGGAAAGAATCATTCCGTGGGCCGCCATCCCAAGCTCTGGTACACGGCTGCCCACCACACATCACTGGAT CAAAGCTCTCCACCCCTCAGTGGAACGTCTCCATCCTACAAGTACACATTACCAGGAGCTCAAGATAGCA AGGATGATTTCCCCTTGAGAAAAACTGCCTCAGAGCCCAACTTGAAGGTGCGGTCCAGGTTAAAACAGAA AGTGGCAGAGAGGAGAAGCAGTCCCTTACTCAGGCGGAAGGATGGAAATCTTGTCACTTCATTCAAGAAG CGAGTGTTTGAGGTGGCAGAATCCTCGGTCAGTAGCAGCTCTCCAGGGTCAGGTCCCAGTTCACCAAACA ATGGCCCTGCTGGGAATGTGACCGAAAATGAGGCTTCAGCTCTGCCTCCCACGCCTCACCCCGAGCAACT GGTTCCACAGCAGCGCATACTAATTCATGAAGATTCCATGAACCTGCTAAGTCTCTATACCTCCCCGTCC CTGCCCAATATCACTCTGGGACTTCCAGCAGTGCCGTCCCCACTCAATGCTTCTAACTCACTCAAAGACA AACAGAAGTGCGAGACACAGATGCTCAGACAAGGTGTTCCTCTGCCCAGTCAGTATGGCAGTAGCATTGC AGCGTCCTCCAGCCACGTTCATGTAGCAATGGAAGGAAAACCCACCAGCAGCCACCAGGCTCTCCTGCAG CACCTACTGCTGAAGGAACAAATGCGACAGCAAAAGCTTCTCGTGGCTGGTGGAGTTCCATTACACCCTC AGTCTCCTTTGGCAACAAAAGAAAGAATTTCACCAGGCATTAGAGGTACCCACAAATTGCCCCGCCACCG ACCCCTGAATCGAACCCAGTCTGCACCTTTGCCTCAGAGCACGCTGGCTCAGCTGGTCATTCAGCAGCAG CACCAGCAGTTCCTGGAGAAGCAGAAACAATACCAGCAGCAGATCCACATGAACAAACTGCTATCGAAAT CTATTGAACAACTGAAGCAACCAGGCAGCCACCTTGAAGAAGCAGAGGAGGAGCTTCAGGGGGACCAATC CATGGAAGACAGAGCAGCCTCTAAGGACAACAGTGCTAGGAGTGACAGCAGTGCTTGTGTGGAGGACACG CTGGGACAAGTTGGGGCTGTGAAGGTCAAGGAGGAACCCGTGGACAGCGATGAAGATGCTCAGATACAGG AAATGGAATGTGGGGAGCAGGCTGCTTTTATGCAACAGCCTCCCTTGCTGGAGTCCTCACGCGCGCGCTC ACTCTCCGTGCACCAGACTCTGGCTGCAGTTGGCACCGATGGATTAGAGAAACATGGTCTCCTCTCCCGG ACCCATTCTTCTCCTGCAGCCTCCATGTCACCTCACCCAGCAGCCGACTGTCCCCGCCAGCCTTCCTCTG CCACTGGAATTGCCTATGACCCTCTGATGCTGAAACACCAGTGCATCTGTGGCAGCTCCACGACCCACCC TGAGCACGCCGGGCGGATACAGAGCATCTGGTCACGGCTGCAAGAAACGGGGCTGCTAAGTAAATGTGAG CGAATTCAAGGTCGAAAAGCCAGCCTGGAGGAAATACAGCTTGTTCATTCTGAACATCACTCACTATTGT ATGGCACCAGCCCCCTGGATGGACAGAAGCTGGACCCCAGGACACTCTTAGGTGATGATTCTCGGAAATT CTTTTCCTCGTTGCCTTGTGGTGGACTCGGGGTGGACAGTGACACCATTTGGAATGAGCTGCACTCGTCC GGTGCTGCACGCATGGCTGTTGGCTGTGTCATTGAGCTGGCTTCCAAAGTGGCCTCAGGAGAGCTGAAGA ATGGGTTTGCCGTTGTGAGGCCCCCAGGCCATCATGCGGAAGAGTCTGCAGCCATGGGGTTCTGCTTTTT TAATTCAGTTGCAATTACCGCCAAATACTTGAGAGACCAACTAAATATAAGCAAGATATTGATTGTAGAT CTGGATGTTCACCATGGAAACGGTACACAGCAGGCCTTTTATGCTGACCCCAGCATCCTGTACATTTCAC TCCATCGCTATGATGAAGGGAACTTTTTCCCTGGCAGTGGAGCCCCGAATGAGGTTGGAGTAGGTCTGGG AGAAGGCTACAATGTAAACATTGCCTGGACAGGTGGCCTCGACCCCCCTATGGGAGATGTTGAGTACCTT GAAGCGTTCAGGACTGTCGTGATGCCTGTGGCCAGAGAGTTTGATCCAGATATGGTCCTGGTTTCTGCTG GATTTGATGCATTGGAAGGCCATACCCCTCCCCTCGGGGGTTACAAAGTGACAGCAAAATGCTTTGGTCA TTTGACGAAGCAACTGATGACATTGGCTAATGGACGTGTGGCATTGGCTCTGGAAGGAGGACATGATCTG ACAGCTATTTGTGATGCATCGGAAGCCTGCATAAATGCCCTTCTAGGAAATGAGCCTGGTCCTCTTGAGG AAGATGCTCTCCACCAGTCCGTGAATACGAATGCAGCCGCTTCTTTACAGAAGACCATTGACATCCAAAG CAAGTATTGGAAGTCAATCAAGATGGTGGCTGCTGCAAGGGGCGGTGCTCTGCCTGCTTCTCAGCTGCAA GAGGAGACAGAGACCGTTTCTGCGCTAGCCTCCCTGACGGTAGATGTGGAACAGCCATTTGCTCAGGAAG ATGGCAGAACTGCTGGTGAGCCCATGGAAGAGGAGCCAGCCTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271386
Insert Size	3267 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271386.1, NP_001258315.1
RefSeq Size	8195 bp
RefSeq ORF	3267 bp
Locus ID	79221
Gene Summary	Devoided of intrinsic deacetylase activity, promotes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) by recruiting HDAC1 and HDAC3. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription, inhibits skeletal myogenesis and may be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. This variant lacks full-length transcript support the exon combination is inferred from partial transcripts and orthologous alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.