Slc12a4 (NM_001253804) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW546649; KCC1; RBCKCC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229352 representing NM_001253804 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCACTTCACCGTGGTGCCGGTGGACGGGCCGCGACGCGGCGACTATGACAACCTCGAGGGGCTCA GTTGGGTGGACTACGGCGAGCGCGCCGAGCGGGAAGACTCGGACGGTGAGGGACAGGGTAACCACAGAGA GAATAGCCCTTTCCTTTGCCCTTTGGACGCCTCCAGAGGAAATGACTACTATGACCGGAACTTGGCACTG TTTGAGGAGGAGCTGGACATCCGCCCAAAGGTGTCATCTCTCCTGGGCAAGCTTGTCAGCTATACCAACC TCACCCAAGGAGCCAAGGAGCACGAGGAGGCTGAGAGTGGAGAAGGTGGCCGACGGAGAGCTGCCAAGGC TCCCAGCATGGGCACACTCATGGGAGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTCATCCTCTTC CTGCGGCTGACCTGGATGGTGGGCACAGCTGGTGTGCTGCAGGCTCTCCTCATTGTCCTCATCTGCTGCT GCTGTACCCTGCTGACAGCCATCTCCATGAGCGCCATTGCCACCAACGGCGTGGTTCCAGCTGGTGGCTC GTACTTCATGATTTCCCGCTCTTTGGGACCAGAATTCGGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGG ACGACATTTGCAGCAGCCATGTATATCCTAGGGGCCATTGAGATCTTGCTGACCTACATTGCTCCGCCAG CTGCCATCTTTTACCCATCGGGCACCCATGACATGTCAAGCGCCACCTTGAATAACATGAGAGTGTATGG GACCATTTTCCTGACTCTGATGACCCTAGTGGTGTTTGTCGGTGTCAAGTACGTGAACAAGTTTGCCTCG CTCTTCCTGGCCTGCGTGATCATCTCCATCCTTTCCATTTACGCGGGAGGCATCAAGTCCATTTTTGACC CTCCCGTGTTTCCGGTGTGTATGCTGGGCAATAGGACCCTGTCTCGGGACCAGTTTGACATCTGTGCCAA GACAGTCGTGGTGGACAATGAGACAGTGGCCACCCGGCTGTGGACTTTCTTCTGCCACAGCCCCAACCTT ACTGCTGACTCCTGTGACCCCTACTTCCTGCTCAACAATGTGACAGAGATTCCTGGCATACCTGGGGCAG CTGCTGGTGTGCTCCAGGAGAACCTCTGGAGCGCTTACCTGGAGAAGGGGGAGGTCGTGGAGAAGCATGG GCTGCCGTCCACAGACACCCTCGGCCTAAAGGAGAGCCTGTCCCTGTATGTGGTGGCTGACATCGCCACG TCCTTCACCGTGCTGGTTGGCATCTTTTTCCCTTCTGTGACAGGCATCATGGCTGGTTCAAACCGCTCCG GGGATCTCCGTGACGCCCAGAAGTCTATCCCAGTGGGGACCATTCTGGCCATTGTTACCACTTCGCTGGT GTACTTCAGCAGTGTGATCCTCTTCGGTGCCTGCATCGAGGGTGTGGTACTCCGGGACAAGTACGGTGAT GGCGTCAGCAGGAACCTGGTGGTGGGCACCCTGGCCTGGCCTTCGCCTTGGGTCATCGTGGTTGGCTCCT TCTTCTCAACATGTGGGGCTGGTCTCCAGAGCCTCACTGGGGCGCCACGTTTACTGCAAGCCATTGCCAA GGATAACATCATCCCCTTCCTCCGGGTGTTTGGCCATGGGAAAGCAAACGGTGAACCGACGTGGGCCCTC CTCCTGACAGCGCTCATCGCTGAGCTGGGCATCCTGATTGCTTCCCTTGACATGGTGGCCCCCATTCTGT CCATGTTCTTTCTGATGTGTTACCTCTTTGTAAACTTGGCCTGTGCCGTGCAGACACTTCTGAGGACCCC CAACTGGCGGCCCCGGTTCAAGTACTATCACTGGACATTGTCTTTCCTAGGCATGAGTCTCTGCCTGGCT CTTATGTTTGTCTCCTCCTGGTACTATGCCCTAGTGGCCATGCTCATCGCAGGCATGATCTACAAGTACA TCGAGTACCAAGGGGCCGAGAAGGAGTGGGGCGATGGGATCCGAGGCCTGTCCCTGAGTGCGGCACGCTA TGCACTGCTGAGGCTGGAGGAAGGGCCTCCTCACACCAAGAACTGGCGGCCTCAGCTCCTGGTGCTGCTT AAGTTAGACGAAGATCTTCATGTGAAGTACCCCCGGCTCCTCACCTTCGCCTCCCAGCTGAAGGCCGGGA AGGGCCTGACAATCGTTGGCTCTGTCATCCAGGGCAGCTTTCTGGAGAGCTATGGTGAAGCCCAGGCCGC TGAGCAGACCATCAAGAACATGATGGACATTGAGAAAGTCAAAGGCTTCTGCCAGGTAGTGGTGGCCAGC AAGGTTCGAGAGGGACTGGCCCACCTCATCCAGTCTTGCGGCCTGGGCGGCATGAGACACAACTCCGTGG TGCTGGGCTGGCCCTATGGCTGGCGACAGAGTGAGGACCCACGTGCCTGGAAGACCTTTATTGACACTGT GCGCTGCACCACAGCTGCCCACCTGGCCCTGCTGGTGCCAAAGAACATAGCTTTCTACCCCAGCAACCAC GAGCGCTACCTGGATGGCCATATCGACGTGTGGTGGATCGTGCATGATGGAGGCATGCTTATGCTGCTGC CCTTTCTGCTGCGCCAGCATAAGGTTTGGAAGAAGTGCCGGATGCGCATTTTCACCGTGGCCCAGATGGA TGACAACAGCATCCAGATGAAGAAGGATCTGGCCATCTTCCTGTATCACCTCCGCCTGGAAGCTGAAGTG GAGGTGGTAGAGATGCACAACAGTGACATCTCTGCCTATACCTATGAGCGGACACTGATGATGGAGCAGC GGTCTCAGATGCTGCGGCAGATGAGGTTGACCAAAACTGAGCGGGATCGAGAGGCCCAGCTGGTAAAGGA CAGGCACTCGGCTCTTAGGCTGGAGAGCCTCTACTCCGACGAGGAGGAAGAGTCTGTGGCAGGGGCCGAC AAGATCCAGATGACGTGGACCAGAGACAAGTACATGGCTGAGCCCTGGGACCCCAGCCACGCCCCTGACA ACTTCCGGGAGCTGGTGCACATTAAGCCGGACCAGTCCAACGTGCGGCGCATGCACACTGCTGTGAAGCT CAACGAGGTCATTGTCACGCGCTCCCACGATGCCCGTCTGGTTTTGCTGAACATGCCTGGCCCCCCTAAG AACAGCGAGGGCGACGAGAACTACATGGAATTCCTTGAAGTCCTAACCGAGGGCCTTGAACGGGTGTTGT TGGTGCGTGGCGGCGGCCGGGAAGTCATCACCATCTATTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253804
Insert Size	3264 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253804.1, NP_001240733.1
RefSeq Size	3832 bp
RefSeq ORF	3264 bp
Locus ID	20498
Gene Summary	Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).

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