Cp (NM_001276250) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D3Ertd555; D3Ertd555e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229350 representing NM_001276250 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTTTTGCTGCTTAGCACATTTATATTTTTGTATAGTTCCTTAGCCTTGGCAAGAGATAAGCATT ATTTCATTGGAATTACTGAAGCAGTCTGGGACTATGCTTCTGGCACTGAAGAAAAGAAACTTATTTCAGT TGACACGGAACAGTCCAATTTCTATCTTCAAAATGGTCCAGATCGTATTGGAAGAAAATATAAGAAGGCC CTTTATTTTGAGTACACAGATGGCACCTTTAGTAAGACTATAGACAAACCAGCCTGGCTAGGGTTTTTAG GCCCTGTCATCAAAGCTGAAGTTGAAGATAAAGTTTATGTTCACTTAAAGAACCTTGCCTCTAGGATCTA CACTTTTCATGCACATGGGGTAACGTACACCAAGGAGTATGAGGGAGCCGTCTACCCTGACAACACCACT GATTTTCAACGGGCTGATGACAAAGTGCTTCCCGGACAACAGTATGTGTATGTGCTGCATGCCAATGAGC CAAGTCCTGGAGAGGGAGACAGCAATTGTGTGACCAGGATTTACCACTCCCATGTTGATGCTCCAAAAGA TATTGCATCAGGACTCATAGGACCTCTAATACTCTGTAAAAAAGGTTCTCTATATAAGGAAAAAGAGAAA AATATTGACCAAGAATTTGTACTAATGTTCTCTGTGGTGGATGAAAATCTCAGCTGGTATCTGGAAGATA ACATCAAAACCTTCTGCTCTGAACCCGAGAAAGTTGATAAAGACAATGAAGACTTCCAGGAAAGCAACAG GATGTACTCTATAAATGGATATACATTTGGAAGCCTCCCAGGGCTCTCGATGTGTGCAGCAGACAGAGTG AAGTGGTACCTTTTTGGTATGGGTAATGAAGTTGATGTGCATTCAGCTTTCTTTCATGGCCAAGCCCTGA CCAGCAGGAACTATCAAACCGATATAATCAACCTGTTCCCTGCCACCCTAATTGATGCTTATATGGTGGC CCAGAATCCTGGAGTCTGGATGCTCAGTTGCCAGAACCTAAACCATCTGAAAGCTGGGTTGCAGGCCTTT TTCCAGGTTCGTGACTGTAACAAGCCCTCGCCAGAGGATAATATCCAAGATAGGCATGTGAGACACTATT ACATTGCTGCTGAGGAGGTCATCTGGAATTATGCTCCTTCTGGGACGGACATCTTCACTGGAGAGAATTT AACAGCTCTGGAAAGTGATTCAAGGGTATTTTTTGAGCAAGGTGCCACAAGAATTGGTGGCTCTTATAAA AAAATGGCATATCGTGAGTACACAGATGGTTCCTTCACAAACCGAAAACAAAGAGGCCCTGATGAGGAAC ATCTTGGAATCCTAGGTCCTGTCATTTGGGCAGAAGTAGGAGACACCATTAAAGTCACCTTTCATAACAA AGGACAGCATCCTCTCAGCATTCAGCCAATGGGAGTAAGTTTCACTGCAGAAAATGAGGGAACATACTAT GGCCCACCAGGTCGCTCCTCACAGCAAGCCTCCCATGTGGCTCCCAAAGAAACCTTTACATACGAATGGA CTGTCCCCAAAGAAATGGGACCCACTTATGCAGATCCTGTGTGCCTATCTAAGATGTACTACTCTGGCGT TGACCCCACCAAAGATATATTTACTGGGCTTATTGGGCCAATGAAAATATGCAAGAAAGGCAGCTTACTT GCTGATGGGAGACAGAAAGATGTAGACAAAGAGTTCTACTTGTTTCCCACAGTGTTTGATGAGAATGAGA GTTTACTCTTAGATGATAATATCAGGATGTTCACAACTGCACCTGATCAAGTGGATAAGGAAGATGAAGA CTTTCAGGAGTCTAATAAGATGCACTCCATGAATGGGTTCATGTATGGCAATCAGCCTGGCCTCAATATG TGTCTAGGAGAATCCATCGTGTGGTATTTGTTCAGCGCTGGAAATGAGGCTGATGTGCATGGGATATACT TTTCAGGAAATACTTATCTGTCTAAAGGAGAAAGAAGAGACACTGCAAACCTATTCCCTCATAAAAGTCT CACCCTTCTCATGAACCCTGACACAAAAGGGACTTTTGATGTTGAGTGCCTTACAACGGATCACTACACA GGTGGCATGAAGCAAAAATACACTGTGAACCAGTGCCAGCGGCAGTTTGAAGATTTCACTGTCTACCTTG GAGAAAGGACCTACTATGTGGCAGCCGTAGAGGTGGAATGGGATTACTCACCAAGCAGGGCCTGGGAAAA GGAGCTGCATCATTTGCAAGAGCAAAATGTTTCAAATGTATTTTTGGATAAAGAAGAGTTTTTCATAGGC TCAAAGTACAAGAAGGTTGTGTATCGCCAGTTTACTGACAGCTCATTCAGAGAACAGGTGAAGAGACGAG CCGAAGACGAGCACTTGGGCATCCTTGGGCCACCAATTCATGCAAATGTTGGAGACAAAGTTAAAGTTGT CTTTAAAAATATGGCAACCAGGCCATATTCAATACATGCCCATGGGGTGAAAACAGAGAGTTCTACAGTT GTTCCAACGTTACCAGGTGAAGTTCGAACTTATACATGGCAAATTCCAGAAAGATCAGGGGCTGGAAGAG AGGATTCAGCTTGTATCCCATGGGCTTATTACTCAACTGTGGATCGAGTTAAGGATCTCTATAGTGGGCT AATAGGCCCATTGATTGTTTGTCGGAAGTCTTATGTGAAAGTATTCAGTCCTAAAAAGAAAATGGAGTTT TTCCTTCTGTTTCTAGTATTTGATGAGAATGAATCTTGGTACTTAGATGATAACATCAAAACATACTCTG AACACCCTGAGAAAGTAAACAAAGACAACGAGGAATTCCTAGAAAGCAATAAAATGCATGCTATTAATGG GAAAATGTTTGGAAACCTACAAGGCCTCACAATGCACGTGAAAGATGAAGTCAACTGGTATGTGATGGGA ATGGGCAATGAAATAGACCTGCACACTGTACACTTCCACGGCCACAGCTTCCAATACAAGCACAGGGGAG TATACAGTTCTGATGTCTTTGACCTTTTCCCTGGAACATACCAAACCCTAGAAATGTTTCCCCAAACACC TGGAACCTGGTTACTCCACTGCCACGTGACTGACCATGTCCATGCTGGGATGGCAACTACCTACACTGTT TTACCAGTAGAACAAGTATCATCTCAGAGTTACAGGATGACCTGGAACATCCTCTATACACTACTAATCA GCATCATTATTTTATTCCAAATGTCTACCAAGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276250
Insert Size	3258 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276250.1, NP_001263179.1
RefSeq Size	4712 bp
RefSeq ORF	3258 bp
Locus ID	12870
Gene Summary	The protein encoded by this gene is a copper-containing glycoprotein found soluble in the serum and GPI-anchored in other tissues. It oxidizes Fe(II) to Fe(III) and is proposed to play an important role in iron homeostasis. In humans mutations of this gene cause aceruloplasminemia, which is characterized by retinal degeneration, diabetes, anemia and neurological symptoms. In mouse deficiency of this gene in combination with a deficiency of its homolog hephaestin causes retinal degeneration and serves as a pathophysiological model for aceruloplasminemia and age-related macular degeneration. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (4) uses an alternate, in-frame acceptor splice site as well as an alternate 3' exon structure, compared to variant 2. It encodes isoform c which is longer and has a distinct C-terminus, compared to isoform b. Both variants 3 and 4 encode the same isoform (c). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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