Xpo1 (NM_134014) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA420417; Crm1; Exp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229335 representing NM_134014 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCAATTATGACAATGTTAGCAGACCATGCAGCTCGTCAGCTGCTTGATTTCAGCCAAAAACTGG ATATCAACCTTTTAGACAACGTGGTGAATTGCTTATACCATGGAGAAGGAGCCCAGCAAAGAATGGCTCA AGAAGTACTGACACATTTAAAGGAGCATCCTGATGCTTGGACACGGGTCGACACAATATTGGAGTTTTCG CAGAACATGAACACGAAATACTATGGACTACAGATTTTGGAAAATGTGATCAAAACAAGGTGGAAGATCC TTCCAAGGAACCAGTGTGAAGGAATAAAAAAATATGTTGTTGGTCTCATTATCAAGACATCCTCTGACCC AACTTGTGTAGAGAAAGAAAAGGTGTATATTGGGAAATTGAATATGATTCTTGTTCAGATACTTAAGCAA GAGTGGCCTAAGCATTGGCCAACTTTTATCAGTGACATTGTTGGAGCGAGTAGGACCAGTGAAAGTCTAT GTCAAAATAATATGGTGATTCTTAAACTCTTGAGTGAAGAAGTGTTTGATTTCTCTAGTGGACAGATAAC CCAAGTTAAAGCAAAGCATTTAAAAGACAGCATGTGCAATGAGTTTTCACAAATATTTCAGCTTTGTCAG TTTGTGATGGAAAATTCCCAAAATGCTCCCCTTGTTCATGCTACATTAGAAACACTGCTGAGATTTCTGA ACTGGATTCCACTGGGATACATCTTTGAGACCAAGTTAATCAGCACATTAATTTACAAGTTCCTGAATGT TCCAATGTTCCGAAATGTGTCTCTAAAGTGCCTCACTGAGATTGCTGGTGTGAGTGTAAGCCAATATGAG GAACAATTTGAAACTCTGTTTACACTTACAATGATGCAGCTAAAACAGATGCTTCCTTTAAACACTAATA TCCGACTTGCATACTCGAATGGGAAAGATGATGAACAGAACTTTATTCAAAATCTCAGCCTGTTTCTCTG TACCTTTCTAAAAGAGCACGGTCAACTTCTAGAAAAACGACTGAATCTCAGGGAAGCACTCATGGAGGCT CTTCATTATATGTTGTTGGTGTCTGAAGTGGAAGAAACTGAAATTTTTAAAATCTGCCTTGAGTACTGGA ATCATTTGGCAGCTGAGCTCTACAGAGAGAGTCCATTTTCTACATCTGCTTCTCCCTTGTTATCTGGAAG TCAACACTTTGATATTCCTCCTAGGAGACAGCTGTACTTGACCGTGTTATCAAAGGTCCGTTTATTGATG GTTAGTCGCATGGCTAAACCAGAGGAGGTACTGGTTGTAGAAAATGATCAGGGAGAAGTTGTAAGAGAGT TCATGAAGGATACTGATTCCATAAATTTATATAAGAATATGAGAGAAACATTAGTTTATCTTACTCATCT GGATTATGTAGATACGGAAATAATAATGACTAAGAAACTTCAAAATCAAGTGAATGGTACAGAATGGTCA TGGAAGAATTTGAATACGTTGTGTTGGGCAATAGGCTCCATCAGTGGCGCAATGCATGAAGAGGATGAGA AGCGGTTTCTTGTTACTGTTATAAAGGATCTTTTAGGATTATGTGAGCAGAAAAGAGGCAAAGATAATAA AGCTATTATTGCATCGAATATCATGTACATAGTAGGTCAATACCCAAGATTTTTGAGAGCACACTGGAAA TTTTTGAAGACAGTAGTAAACAAGCTATTCGAATTCATGCATGAGACCCATGATGGAGTCCAGGATATGG CTTGTGACACATTTATTAAAATAGCTCAAAAATGCCGTAGGCATTTTGTTCAGGTTCAAGTTGGAGAAGT AATGCCGTTCATTGATGAGATTTTAAACAACATAAATACTATAATTTGTGATCTTCAGCCTCAGCAGGTT CATACATTTTATGAAGCTGTGGGGTACATGATTGGTGCACAGACAGACCAGACAGTTCAAGAGCATTTGA TAGAAAAATACATGCTACTCCCTAATCAAGTGTGGGACAGTATAATTCAGCAGGCTACCAAAAATGTGGA TATCCTGAAAGATCCTGAAACAGTCAAGCAGCTGGGTAGCATATTAAAAACAAATGTGAGAGCCTGCAAA GCTGTTGGTCATCCCTTTGTAATTCAGCTTGGCAGAATTTATTTAGACATGCTTAATGTATATAAGTGCC TCAGTGAAAATATTTCTGCAGCTATTCAAGCTAATGGTGAGATGGTTACAAAGCAACCATTGATTAGAAG TATGCGGACAGTAAAAAGGGAAACTTTAAAGTTAATATCTGGTTGGGTGAGCCGTTCCAATGATCCTCAG ATGGTAGCTGAAAACTTTGTTCCTCCTTTGTTGGATGCAGTTCTTATTGATTACCAGAGAAATGTACCAG CTGCTAGAGAACCAGAAGTGCTTAGTACTATGGCCATTATTGTCAACAAATTAGGTGGACATATAACAGC TGAAATACCTCAAATATTCGATGCAGTTTTTGAGTGCACATTGAATATGATAAATAAGGATTTTGAAGAG TATCCAGAACACAGAACAAACTTTTTCTTATTACTTCAAGCTGTCAATTCTCACTGTTTCCCAGCATTCC TAGCTATTCCACCTGCACAGTTCAAGCTGGTTTTGGATTCTATTATTTGGGCTTTTAAACATACTATGAG GAATGTTGCAGATACAGGCTTACAGATACTTTTTACACTTTTACAAAATGTTGCACAAGAGGAAGCTGCC GCACAGAGTTTTTATCAAACTTATTTTTGTGATATTCTTCAGCATATTTTCTCTGTTGTAACAGACACTT CACATACTGCAGGTCTAACAATGCATGCATCAATTCTTGCATATATGTTTAATTTGGTTGAAGAAGGAAA AATAAGTACACCACTAAATCCTGGAAATCCAGTTAACAACCAAATGTTTATTCAAGACTATGTGGCTAAT CTCCTTAAATCTGCATTCCCTCATCTACAAGATGCTCAAGTAAAGCTCTTTGTAACAGGACTTTTCAGCT TAAATCAGGATATTCCTGCTTTCAAAGAACACCTAAGGGATTTCTTAGTTCAAATAAAGGAGTTTGCAGG TGAAGATACATCGGATCTATTTTTGGAAGAAAGAGAAACAGCCCTTCGACAGGCTCAGGAGGAGAAACAT AAACTTCAAATGTCTGTACCTGGGATTCTTAATCCACACGAAATTCCAGAAGAAATGTGTGATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_134014
Insert Size	3216 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_134014.3, NP_598775.2
RefSeq Size	5144 bp
RefSeq ORF	3216 bp
Locus ID	103573
UniProt ID	Q6P5F9
Gene Summary	Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).[UniProtKB/Swiss-Prot Function]

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