Daam1 (NM_001286452) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229332 representing NM_001286452 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCACGGAAGAGAGGCGGCCGGGGGATTTCATTTATCTTTTGCTGTTTCCGAAACAATGACCATC CAGAAATCACATATCGGCTTAGGAATGATAGCAACTTTGCACTTCAGACAATGGAGCCGGCGCTGCCCAT GCCCCCTGTGGAGGAGCTCGATGTCATGTTCAGTGAACTTGTGGATGAACTTGACCTCACAGACAAACAC AGGGAAGCCATGTTTGCACTTCCAGCAGAGAAAAAGTGGCAGATATACTGTAGCAAGAAAAAGGACCAGG AAGAAAACAAGGGAGCAACCAGTTGGCCCGAGTTCTACATTGATCAGCTCAATTCCATGGCTGCTAGGAA ATCTCTGCTGGCTTTAGAGAAGGAAGAAGAGGAGGAGAGGAGTAAAACCATCGAGAGTCTGAAGACAGCA CTGAGAACCAAGCCCATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTGTCATGTATTCTCAACT TTCTGAAGACCATGGACTATGAGACCTCTGAGTCTCGGATACACACCTCCCTCATTGGCTGTATAAAGGC ACTAATGAACAACTCTCAAGGCCGGGCCCATGTCCTGGCTCATTCTGAGAGTATCAATGTAATTGCTCAG AGTCTGAGCACAGAGAACATTAAAACAAAGGTGGCCGTGCTGGAAATCCTGGGCGCTGTGTGCCTGGTTC CTGGGGGCCACAAGAAAGTTCTGCAAGCTATGCTGCACTACCAGAAGTATGCCAGCGAGAGAACCCGCTT CCAGACGTTAATTAATGACTTGGATAAAAGCACTGGGCGTTATCGGGATGAAGTAAGTCTCAAGACGGCC ATCATGTCCTTCATCAATGCAGTGCTGAGCCAGGGAGCCGGAGTGGAGAGTTTGGACTTCAGGCTCCATC TTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTTATAGATAAATTAAGGGAACATGAAAATTCAAC ACTAGACAGGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTT GAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACA GCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAA CACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAG GATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGTTGGTTAATGAAAATG AAGTTAAGCAATGGAAAGAGCAGGCAGAGAAGATGAGGAAAGAGCACAATGAGCTACAACAGAAGCTGGA GAAGAAAGAGAGAGAATGTGATGCTAAGACCCAAGAGAAGGAAGAAATGATGCAGACCTTAAACAAGATG AAAGAGAAGCTGGAAAAGGAGACAACTGAGCACAAGCAAGTTAAGCAGCAGGTGGCAGACCTCACGGCGC AGCTCCACGAGCTGAACAGGAGGGCCGTCTGTGCAGCAGTCCCAGGAGGACCCTCACCCGGAGCCCCTGG AGGACCCTTTCCTTCCTCTGGGTTGGGGTCTCTTCTCCCTCCCCCACCACCCCCACTCCTTTCAGGTGGA GCACTTCCTCCTCCACCACCTCCCCTCCCTCCTGGGGGTCCTCCTCCTCCCCCAGGGCCTCCTCCCTTAG GAGGAGTTCTGCCACCTCCTGGTGCTCCAGTGAGTCTAACACTCAAGAAGAAGAATATTCCCCAGCCCAC CAATGCCCTGAAATCCTTCAACTGGTCCAAGTTGCCTGAGAACAAATTGGATGGAACAGTATGGACTGAA ATTGATGATACCAAAGTCTTCAAAATTCTAGACCTTGAAGACCTAGAAAGAACGTTCTCTGCCTACCAAA GACAGCAGAAAGAAGCAGATGCCATTGATGACACACTGAGTTCCAAACTTAAAGTCAAAGAGCTGTCAGT GATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTGTCGAGGTTGAAATTATCCAATGATGAAATC AAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCAAGGACATGTTAGAACAGCTTTTGAAAT TCGTTCCTGAGAAAAGCGACATCGACCTGCTAGAGGAACACAAGCATGAGCTGGATCGGATGGCCAAGGC TGACCGCTTCCTGTTTGAGATGAGCAGAATTAATCATTACCAGCAAAGACTGCAATCACTGTACTTCAAA AAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAGCCCAAAGTAGAAGCGATTCGCTCTGGCTCTGAGGAGG TGTTCAGGAGCCGCGCCCTCAAGCAGCTCCTGGAGGTGGTCTTGGCTTTTGGAAATTATATGAATAAAGG ACAAAGAGGCAATGCATATGGATTCAAGATCTCCAGCCTCAACAAGATAGCGGATACCAAATCCAGTATT GACAAGAACATTACTCTTTTGCACTATCTAATAACTATTGTGGAGAATAAGTACCCCAAAGTCCTCAACC TAAGCGAAGAGTTGCGGGATATTCCTCAAGCAGCGAAAGTAAACATGACTGAGCTGGACAAGGAGATCAG CACCCTGAGGAGTGGCCTGAAAGCTGTAGAGACGGAGCTGGAGTATCAGAAGTCTCAGCCCCCACAGCCT GGAGACAAGTTCGTGTCTGTTGTCAGCCAGTTCATCACCCTGGCTAGCTTCAGCTTCTCAGATGTTGAAG ATCTTCTAGCAGAAGCTAAAGAGCTGTTCACTAAAGCAGTGAAGCACTTTGGGGAAGAGGCTGGCAAGAT CCAGCCAGATGAGTTCTTCGGGATCTTTGATCAGTTTCTTCAGGCCGTGGCCGAAGCGAAACAGGAAAAT GAGAACATGAGAAAAAGGAAGGAGGAGGAGGAACGGCGGGCTCGCCTGGAAGCTCAGCTCAAAGAGCAGC GGGAGAGGGAGCGGAAGGTGAGGAAGGCCAAGGAGAGCAGTGAAGAGAGTGGAGAGTTTGACGACCTGGT CTCGGCGCTGCGCTCAGGAGAGGTGTTCGACAAAGACCTTTCAAAACTCAAACGGAACCGGAAACGCATT TCCAACCAGGTGACGGACAGCAGCCGGGAGCGACCAATCACAAAACTTAATTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286452
Insert Size	3207 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286452.1, NP_001273381.1
RefSeq Size	6028 bp
RefSeq ORF	3207 bp
Locus ID	208846
UniProt ID	Q8BPM0
Gene Summary	Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (PubMed:26526197).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b) is shorter, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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