Llgl2 (NM_001252532) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9130006H11Rik; Llglh2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229315 representing NM_001252532 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGTTCCTGAGAACTGGACATGACCCTGCCCGGGAGAGGCTGAAGCGGGATCTGTTCCAGTTTA ACAAGACAGTGGAGCATGGCTTCCCACACCAGCCCAGCGCCCTTGGCTATAGCCCTTCGCTGCGCATCCT GGCCATCGGAACCCGCTCTGGAGCTGTCAAGCTATATGGCGCCCCCGGGGTAGAGTTCATGGGCCTTCAC AAGGAGAACAACGCAGTGCTGCAGATCCACTTCCTGCCTGGTCAGTGTCAGCTGGTCACTCTGCTGGATG ACAATAGCCTGCACCTGTGGAGCCTGAAGGTCAAGGGCGGGGTGTCAGAGCTGCAGGAAGAAGAGAGCTT CACATTGCGTGGCCCCCCAGGGGCTGCCCCCAGTGCCACGCAGGTCACTGAGATTCTACCTCACTCTTCC GGAGAACTGCTCTACCTGGGCACCGAGAGCGGCAACGTGTTTGTGGTGCAGCTCCCGGGGTTCCGCACGC TGCACGACAGGACCATCTGTTCAGATGAGGTGCTGCAATGGTTGCCAGAGGAGGCTCGCCACCGGCGAGT GTTTGAGATGGTGGAGGCTCTGCAGGAGCACCCTCGAGACCCCAACCAAATCCTCATTGGCTACAGCCGA GGCCTCGTCGTCATCTGGGACCTTCAGGGCAGCCGAGCGCTCAGTCATTTCCTCAGCAGCCAGCAACTGG AGAATGCCAGCTGGCAGCGGGATGGCTGCCTGATTGTCACCTGCCACTCCGACGGCAGCCACTGCCAGTG GCCCGTCTCCAGTGACACCCAGAACCCAGAACCTCTGCGCAGCTCTATACCTTACGGTCCCTTTCCTTGC AAAGCTATAACGAAAATCTTCTGGCTCACCACCAGGCAAGGGTTGCCCTTCACCATCTTCCAGGGCGGTA TGCCACGGGCCAGCTATGGGGACCGCCACTGCATCTCAGTGGTCCACAACGGCCAGCAGACGGCCTTCGA CTTCACCTCCCGTGTCATTGACTTCACTGTTCTCTCAGAGGCTGACCCTGCGGCTGCCTTTGATGACCCC TACGCCCTGGTGGTGCTAGCCGAGGAGGAACTGGTAGTCATTGACCTGCAGACACCCGGCTGGCCACCAG TCCAGCTGCCCTACCTGGCTTCCTTGCACTGTTCCGCTATCACCTGCTCCCACCACGTCTCCAACATCCC CCTGAAGCTATGGGAACGCATCATCGCCGCGGGCAGCCGCCAGAACTCACACTTCTCCACCATGGAGTGG CCCATAGATGGTGGCACCAGCCTGGCCCCACCGCCACCCCAGAGGGACCTGCTGCTCACAGGGCACGAGG ACGGCACGGTGCGGTTCTGGGACGCCTCCGGTGTGTGCTTACGTCTGCTCTACAAACTGAGCACTGTGAG GGTGTTCCTCACAGACACGGACCCCAGCGAGAACCTCATTGCCCAGGGTGAGGACGAGTGGCCCCCACTC CGCAAGGTGGGCTCCTTTGATCCCTACAGCGATGATCCGCGGCTAGGCATCCAGAAGATTTTCCTCTGCA AATACAGCGGCTACCTGGCTGTGGCAGGCACGGCAGGGCAGGTGCTGGTGCTGGAGTTGAACGATGAGGC GGCCGAGCATGCTGTGGAGCAGGTGGAGGCTGACCTGCTGCAGGACCAGGAGGGTTACCGCTGGAAGGGG CACGAGCGCCTCGCCGCCCGCCCGGGGCCCGTGTGCTTTGAGGCGGGATTTCAGCCCTTTGTACTGGTGC AGTGCCAGCCCCCAGCTGTGGTCACCTCCTTGGCTCTGCACTCCGAGTGGCGGCTTGTGGCCTTTGGCAC CAGCCATGGTTTCGGCCTCTTCGATCACCAGCAACGGCGGCAGGTCTTTGTCAAGTGCACACTTCACCCC AGTGACCAGCTGGCCTTGGAGGGCCCTCTGTCTCGAGTAAAGTCCCTCAAGAAGTCTCTACGTCAGTCAT TCCGTCGGATGCGTCGCAGCAGAGTGTCCAGTCATAAACGGCGGCCTGGTGGCCCCACAGGCGAGGTGAG GCCCCAGGCTCAGAGCAGCCATCTACAGGACCATACAGCTCTCCCTCAGGATCTGAATCCAGATCAAGCG CAGGCCCAGGCTGTGAATATCAAGGCTGAGCGGACGGGCCTGCAGAACATGGAGTTGGCGCCCGTGCAAC GGAAGATTGAGGCTCGCTCCGCGGAGGACTCCTTCACTGGCTTTGTCCGGACCCTCTATTTCGCTGATAC CTACCTGAGGGACAGTTCCCGCCACTGTCCCTCCCTGTGGGCTGGCACCAACGGAGGCACCGTCTACGCC TTTTCCCTGCGCGTGCCTCCTGCAGAAAGAAGAACAGATGAACCTGTCCGGGCAGAGCAGGCCAAGGAGA TCCAGCTGATGCATCGCGCGCCTGTGGTGGGCATCCTGGTGCTCGACGGACACAACGTGCCCCTCCCCGA GCCCCTGGAAGTCGCCCATGACCTGTCGAAGAGCCCAGACATGCAAGGGAGCCACCAATTGCTTGTGGTG TCGGAGGAGCAATTCAAGGTATTCACACTGCCCAAGGTGAGCGCCAAACTGAAGCTGAAGCTGACGGCCC TGGAGGGCTCACGGGTGCGGCGAGTCGGTGTCGCCCACTTTGGCAGCTGCAGGGCTGAGGACTATGGGGA GCACCACCTGGCAGTGCTCACCAACTTGGGTGACATCCAGGTGGTCTCAATGCCCCTGCTCAAGCCCCAG GTGCGGTACAGCTGCATCCGCCGGGAGGACGTCAGCGGCATAGCTTCCTGTGTCTTCACCAAATATGGCC AAGGTTTCTATCTGATATCACCTTCGGAGTTCGAGCGCTTTTCTCTCTCCACCAAGTGGCTGGTTGAACC CCGGTGTTTGGTGGATTCAACCAAAGCCAAGAAGCACAACCGGCCCAGCAATGGCAATGGCACAGGCCTC AAAATGACCTCCTCAGGCCACGTCAGGAACTCAAAGAGCCAAAGTGATGGAGATGAGAAGAAGCCGGGCC CAGTGATGGAGCACGCGCTGCTCAATGACGCCTGGGTCCTAAAGGAAATCCAGAGCACGCTGGAGGGGGA CCGGAGGAGCTATGGCAATTGGCATCCTCACCGCGTGGCTGTGGGTTGCAGGCTCAGCAATGGAGAAGCA GAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252532
Insert Size	3156 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252532.1, NP_001239461.1
RefSeq Size	3629 bp
RefSeq ORF	3156 bp
Locus ID	217325
UniProt ID	Q3TJ91
Gene Summary	Part of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the initial phase of the establishment of epithelial cell polarity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate splice site in the central coding region, which does not affect the reading frame, compared to variant 1. The resulting isoform (2) has an additional segment, compared to isoform 1.

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