Ogdh (NM_001252283) Mouse Untagged Clone

CAT#: MC229297

Ogdh (untagged) - Mouse oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), transcript variant 2



  "NM_001252283" in other vectors (1)

CNY 9,980.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2210403E04Rik; 2210412K19Rik; AA409584; d1401; mKIAA4192
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229297 representing NM_001252283
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT
CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA
ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA
AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC
AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCCCATGCACAGTCCCTGGTGGAAGCACA
ACCTAACGTCGACAAACTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCATATCAGGTCAGG
GGTCACCACATTGCAAAACTTGATCCTCTCGGAATTAGTTGTGTAAATTTTGATGATGCTCCGGTAACTG
TTTCTTCAAACGTGGATCTTGCAGTTTTCAAGGAACGACTTCGAATGCTAACAGTAGGAGGGTTCTATGG
CCTACACGAGTCTGACCTTGACAAGGTCTTCCACTTACCCACCACCACTTTCATCGGGGGACAGGAGCCA
GCACTTCCTCTTCGGGAGATCATCCGTCGGCTGGAGATGGCCTACTGCCAGCACATTGGTGTGGAGTTCA
TGTTCATTAATGATTTGGAACAATGCCAGTGGATCCGACAGAAGTTTGAGACCCCTGGAATCATGCAGTT
CACCAATGAGGAGAAGCGGACCTTGCTGGCCAGGCTTGTACGATCCACCAGGTTTGAGGAGTTCCTACAG
CGAAAGTGGTCCTCGGAGAAGCGTTTTGGTCTGGAAGGCTGTGAGGTGCTGATCCCTGCCCTCAAGACAA
TCATTGATATGTCAAGTGCAAATGGAGTGGACTATGTGATCATGGGGATGCCACACAGAGGACGGCTGAA
CGTGCTTGCAAATGTCATCAGGAAGGAGCTGGAGCAAATATTCTGTCAGTTTGACTCAAAGCTGGAGGCA
GCTGATGAGGGTTCTGGGGACATGAAGTACCACCTGGGCATGTATCACCGCAGGATCAACCGTGTGACCG
ACAGAAACATCACTTTGTCCTTGGTGGCTAACCCTTCCCATCTAGAGGCTGCTGACCCTGTCGTGATGGG
AAAGACCAAAGCTGAACAGTTCTACTGTGGAGACACTGAAGGGAAAAAGGTGATGTCTATCCTGCTGCAT
GGGGATGCTGCTTTTGCTGGCCAGGGCATCGTATATGAGACCTTCCATCTCAGCGACTTGCCGTCCTACA
CAACCCATGGCACTGTTCATGTGGTTGTCAACAACCAGATTGGCTTCACCACAGACCCTCGGATGGCCCG
CTCCTCTCCCTACCCCACTGATGTGGCCCGAGTGGTGAATGCCCCCATTTTCCATGTCAACTCAGATGAC
CCTGAAGCTGTCATGTATGTATGCAAGGTGGCAGCTGAGTGGAGAAACACCTTCCACAAGGATGTTGTAG
TTGATCTGGTGTGTTATCGACGAAATGGCCACAATGAGATGGACGAACCTATGTTTACACAGCCACTCAT
GTACAAGCAGATCCGCAAGCAGAAGCCTGTACTGCAGAAGTATGCAGAATTGCTAGTCTCCCAGGGTGTC
GTCAATCAGCCTGAGTACGAGGAGGAAATCTCCAAGTATGATAAGATCTGTGAGGAAGCATTTACCAGAT
CCAAAGATGAGAAGATCTTGCACATCAAGCACTGGCTGGATTCCCCCTGGCCTGGCTTTTTCACCCTGGA
TGGACAGCCCAGGAGCATGACCTGCCCCTCCACTGGCCTGGAGGAGGATGTCTTGTTCCACATTGGAAAG
GTGGCCAGCTCTGTACCTGTGGAGAACTTTACTATCCATGGAGGGCTGAGCCGGATCTTGAAGACCCGCA
GAGAGCTTGTGACGAACCGGACTGTGGACTGGGCCCTGGCAGAGTACATGGCATTTGGCTCACTGCTGAA
GGAAGGCATCCATGTGCGGCTGAGTGGCCAGGATGTGGAGCGGGGCACCTTCAGCCATCGCCACCATGTG
CTCCATGATCAGAATGTTGACAAAAGAACCTGCATCCCCATGAACCACCTTTGGCCAAATCAGGCCCCTT
ACACTGTATGCAACAGCTCGCTGTCTGAGTACGGTGTCCTGGGCTTTGAGCTGGGCTTTGCCATGGCTAG
CCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTTTGGTGACTTCAACAACATGGCACAGTGCATCATTGAC
CAGTTCATCTGCCCAGGACAGGCAAAGTGGGTGCGGCAGAATGGCATTGTGCTCCTGCTGCCTCATGGCA
TGGAAGGCATGGGTCCCGAGCATTCCTCTGCCCGCCCAGAGCGGTTTCTGCAGATGTGCAATGATGACCC
AGATGTCCTGCCTGACCTGCAGGAAGAAAACTTTGACATCAATCAGCTCTATGACTGCAACTGGATTGTT
GTCAACTGTTCCACCCCTGGCAACTTCTTCCATGTGCTGCGACGACAGATCTTGCTGCCCTTCCGGAAGC
CGTTAATCGTCTTCACTCCCAAATCCCTCCTGCGCCACCCTGAGGCAAGAACTAGCTTTGACGAGATGCT
GCCAGGAACGCACTTCCAGCGTGTGATCCCAGAAAATGGACCTGCAGCTCAGGACCCACACAAAGTCAAG
AGACTTCTCTTCTGCACTGGGAAGGTGTACTATGACCTCACCCGAGAGCGCAAAGCCAGGAACATGGAGG
AGGAGGTGGCTATTACAAGGATTGAGCAGCTATCACCATTCCCCTTTGACCTCCTGCTGAAAGAGGCACA
GAAGTATCCCAATGCTGAGCTGGCCTGGTGCCAGGAAGAGCACAAGAACCAAGGCTACTATGACTATGTC
AAGCCAAGACTTCGTACCACCATTGACCGTGCTAAGCCTGTCTGGTATGCTGGCCGAGACCCGGCAGCTG
CTCCAGCCACTGGCAACAAGAAAACACACCTGACAGAGCTGCAGCGCTTTCTGGACACAGCCTTTGACCT
GGACGCATTCAAGAAATTCTCTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001252283
Insert Size 3105 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001252283.1, NP_001239212.1
RefSeq Size 6663 bp
RefSeq ORF 3105 bp
Locus ID 18293
UniProt ID Q60597
Gene Summary 2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) has an alternate coding exon, compared to variant 1. The resulting isoform (2) has a shorter and different internal segment, compared to isoform 1. Variants 2, 6 and 7 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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