Ogdh (NM_001252283) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210403E04Rik; 2210412K19Rik; AA409584; d1401; mKIAA4192
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229297 representing NM_001252283 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCCCATGCACAGTCCCTGGTGGAAGCACA ACCTAACGTCGACAAACTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCATATCAGGTCAGG GGTCACCACATTGCAAAACTTGATCCTCTCGGAATTAGTTGTGTAAATTTTGATGATGCTCCGGTAACTG TTTCTTCAAACGTGGATCTTGCAGTTTTCAAGGAACGACTTCGAATGCTAACAGTAGGAGGGTTCTATGG CCTACACGAGTCTGACCTTGACAAGGTCTTCCACTTACCCACCACCACTTTCATCGGGGGACAGGAGCCA GCACTTCCTCTTCGGGAGATCATCCGTCGGCTGGAGATGGCCTACTGCCAGCACATTGGTGTGGAGTTCA TGTTCATTAATGATTTGGAACAATGCCAGTGGATCCGACAGAAGTTTGAGACCCCTGGAATCATGCAGTT CACCAATGAGGAGAAGCGGACCTTGCTGGCCAGGCTTGTACGATCCACCAGGTTTGAGGAGTTCCTACAG CGAAAGTGGTCCTCGGAGAAGCGTTTTGGTCTGGAAGGCTGTGAGGTGCTGATCCCTGCCCTCAAGACAA TCATTGATATGTCAAGTGCAAATGGAGTGGACTATGTGATCATGGGGATGCCACACAGAGGACGGCTGAA CGTGCTTGCAAATGTCATCAGGAAGGAGCTGGAGCAAATATTCTGTCAGTTTGACTCAAAGCTGGAGGCA GCTGATGAGGGTTCTGGGGACATGAAGTACCACCTGGGCATGTATCACCGCAGGATCAACCGTGTGACCG ACAGAAACATCACTTTGTCCTTGGTGGCTAACCCTTCCCATCTAGAGGCTGCTGACCCTGTCGTGATGGG AAAGACCAAAGCTGAACAGTTCTACTGTGGAGACACTGAAGGGAAAAAGGTGATGTCTATCCTGCTGCAT GGGGATGCTGCTTTTGCTGGCCAGGGCATCGTATATGAGACCTTCCATCTCAGCGACTTGCCGTCCTACA CAACCCATGGCACTGTTCATGTGGTTGTCAACAACCAGATTGGCTTCACCACAGACCCTCGGATGGCCCG CTCCTCTCCCTACCCCACTGATGTGGCCCGAGTGGTGAATGCCCCCATTTTCCATGTCAACTCAGATGAC CCTGAAGCTGTCATGTATGTATGCAAGGTGGCAGCTGAGTGGAGAAACACCTTCCACAAGGATGTTGTAG TTGATCTGGTGTGTTATCGACGAAATGGCCACAATGAGATGGACGAACCTATGTTTACACAGCCACTCAT GTACAAGCAGATCCGCAAGCAGAAGCCTGTACTGCAGAAGTATGCAGAATTGCTAGTCTCCCAGGGTGTC GTCAATCAGCCTGAGTACGAGGAGGAAATCTCCAAGTATGATAAGATCTGTGAGGAAGCATTTACCAGAT CCAAAGATGAGAAGATCTTGCACATCAAGCACTGGCTGGATTCCCCCTGGCCTGGCTTTTTCACCCTGGA TGGACAGCCCAGGAGCATGACCTGCCCCTCCACTGGCCTGGAGGAGGATGTCTTGTTCCACATTGGAAAG GTGGCCAGCTCTGTACCTGTGGAGAACTTTACTATCCATGGAGGGCTGAGCCGGATCTTGAAGACCCGCA GAGAGCTTGTGACGAACCGGACTGTGGACTGGGCCCTGGCAGAGTACATGGCATTTGGCTCACTGCTGAA GGAAGGCATCCATGTGCGGCTGAGTGGCCAGGATGTGGAGCGGGGCACCTTCAGCCATCGCCACCATGTG CTCCATGATCAGAATGTTGACAAAAGAACCTGCATCCCCATGAACCACCTTTGGCCAAATCAGGCCCCTT ACACTGTATGCAACAGCTCGCTGTCTGAGTACGGTGTCCTGGGCTTTGAGCTGGGCTTTGCCATGGCTAG CCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTTTGGTGACTTCAACAACATGGCACAGTGCATCATTGAC CAGTTCATCTGCCCAGGACAGGCAAAGTGGGTGCGGCAGAATGGCATTGTGCTCCTGCTGCCTCATGGCA TGGAAGGCATGGGTCCCGAGCATTCCTCTGCCCGCCCAGAGCGGTTTCTGCAGATGTGCAATGATGACCC AGATGTCCTGCCTGACCTGCAGGAAGAAAACTTTGACATCAATCAGCTCTATGACTGCAACTGGATTGTT GTCAACTGTTCCACCCCTGGCAACTTCTTCCATGTGCTGCGACGACAGATCTTGCTGCCCTTCCGGAAGC CGTTAATCGTCTTCACTCCCAAATCCCTCCTGCGCCACCCTGAGGCAAGAACTAGCTTTGACGAGATGCT GCCAGGAACGCACTTCCAGCGTGTGATCCCAGAAAATGGACCTGCAGCTCAGGACCCACACAAAGTCAAG AGACTTCTCTTCTGCACTGGGAAGGTGTACTATGACCTCACCCGAGAGCGCAAAGCCAGGAACATGGAGG AGGAGGTGGCTATTACAAGGATTGAGCAGCTATCACCATTCCCCTTTGACCTCCTGCTGAAAGAGGCACA GAAGTATCCCAATGCTGAGCTGGCCTGGTGCCAGGAAGAGCACAAGAACCAAGGCTACTATGACTATGTC AAGCCAAGACTTCGTACCACCATTGACCGTGCTAAGCCTGTCTGGTATGCTGGCCGAGACCCGGCAGCTG CTCCAGCCACTGGCAACAAGAAAACACACCTGACAGAGCTGCAGCGCTTTCTGGACACAGCCTTTGACCT GGACGCATTCAAGAAATTCTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252283
Insert Size	3105 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252283.1, NP_001239212.1
RefSeq Size	6663 bp
RefSeq ORF	3105 bp
Locus ID	18293
UniProt ID	Q60597
Gene Summary	2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate coding exon, compared to variant 1. The resulting isoform (2) has a shorter and different internal segment, compared to isoform 1. Variants 2, 6 and 7 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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