Myo1c (NM_001080774) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C80397; mm1bet; mm1beta; MMIb; MYO1E; myr; myr2; NMI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229286 representing NM_001080774 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCGCCTTGACTGCCCGAGACCGGGTAGGGGTGCAGGACTTTGTCCTGCTGGAGAATTTCACCA GTGAGGCTGCCTTCATTGAGAACCTCCGGCGGCGGTTCCGGGAGAACCTCATTTATACCTACATCGGTCC TGTCCTAGTCTCTGTCAATCCCTACCGAGACCTACAGATCTACAGCCGGCAGCATATGGAACGCTACCGT GGTGTCAGTTTCTATGAAGTACCACCTCATTTGTTTGCAGTGGCTGACACTGTATACCGGGCACTTCGTA CTGAGCGTCGGGACCAGGCAGTGATGATTTCTGGAGAGAGTGGGGCAGGCAAGACAGAGGCCACCAAGAG ACTGCTCCAGTTCTATGCAGAGACCTGCCCAGCCCCTGAACGGGGTGGCGCAGTGCGAGACCGCCTGTTG CAGAGCAACCCCGTGTTAGAGGCCTTTGGGAATGCCAAGACTCTCCGCAACGATAACTCCAGCCGGTTTG GAAAGTACATGGATGTGCAGTTTGACTTCAAGGGTGCCCCCGTGGGAGGCCACATTCTCAGTTACCTCCT GGAAAAGTCCCGGGTGGTGCACCAAAATCACGGAGAGCGGAACTTCCACGTCTTTTACCAGCTACTGGAG GGGGGCGAGGAGGAGACTCTCCGTCGGCTGGGCTTGGAACGGAACCCCCAGAGCTACTTGTACCTGGTGA AGGGCCAGTGTGCCAAGGTCTCCTCCATCAACGACAAGAGTGACTGGAAGGTTATGAGGAAGGCGCTGTC CGTCATTGACTTCACTGAGGATGAAGTGGAGGACTTGCTCAGCATCGTGGCCAGCGTCCTACATCTGGGC AACATCCACTTTGCTGCTGACGAGGACAGCAATGCCCAGGTTACTACTGAGAACCAGCTCAAATATCTGA CCAGGCTCCTTGGTGTGGAAGGTACAACACTTAGGGAAGCCCTGACCCACAGGAAGATCATCGCCAAGGG GGAAGAGCTCCTGAGCCCACTGAACCTTGAACAGGCGGCATATGCAAGGGATGCGCTTGCCAAGGCTGTG TACAGCCGGACATTCACCTGGCTGGTCAGAAAGATCAATAGGTCACTGGCCTCTAAGGACGCTGAGAGCC CCAGCTGGCGAAGCACCACGGTTCTTGGGCTCCTGGACATTTACGGCTTTGAAGTGTTTCAGCATAACAG CTTCGAGCAGTTCTGCATCAACTACTGCAATGAGAAGCTGCAGCAGCTCTTCATCGAGCTGACTCTCAAG TCGGAGCAGGAGGAATACGAGGCTGAGGGCATCGCGTGGGAACCTGTCCAGTACTTCAACAACAAGATCA TCTGTGACCTGGTAGAGGAGAAGTTCAAGGGCATCATCTCCATCTTGGATGAAGAGTGCCTGCGTCCTGG GGAGGCCACGGACCTGACCTTTCTGGAGAAGTTGGAGGACACTGTCAAGCCCCACCCTCACTTCCTGACG CACAAGCTCGCTGACCAGAAGACCAGGAAATCTCTAGACCGAGGGGAGTTCCGCCTTCTGCATTATGCTG GAGAGGTGACCTACAGTGTGACTGGGTTTCTGGATAAAAACAATGACCTCCTCTTCCGGAACCTGAAGGA GACCATGTGCAGCTCAATGAACCCCATCATGGCCCAGTGCTTTGACAAGAGTGAGCTCAGTGACAAGAAG CGGCCAGAGACGGTGGCCACCCAGTTCAAGATGAGCCTCCTGCAGCTCGTGGAGATCCTGAGGTCTAAGG AGCCTGCCTATATCCGGTGCATCAAGCCAAACGACGCCAAGCAGCCGGGTCGCTTTGATGAGGTGCTCAT CCGACATCAGGTGAAGTACCTGGGACTGATGGAGAATCTGCGCGTGCGCAGAGCTGGCTTTGCCTATCGT CGCAAATATGAGGCTTTCCTGCAGAGGTACAAGTCACTGTGCCCAGAGACATGGCCCATGTGGGCAGGAC GGCCCCAGGATGGTGTGGCCGTGTTGGTCAGACACCTCGGCTACAAGCCAGAAGAGTACAAAATGGGCAG GACTAAGATCTTCATCCGATTTCCCAAGACCTTGTTTGCCACAGAGGACTCCCTGGAAGTCCGGCGGCAG AGTCTAGCCACCAAGATCCAGGCGGCCTGGAGGGGCTTTCATTGGCGACAGAAATTTCTCCGGGTGAAGC GATCAGCCATCTGTATCCAGTCATGGTGGCGTGGCACACTGGGCCGGAGGAAGGCAGCCAAGAGGAAGTG GGCAGCCCAGACCATCCGTCGACTCATCCGTGGCTTCATTTTGCGCCATTCACCCCGGTGCCCTGAGAAT GCCTTCTTCTTGGACCACGTGCGCGCCTCATTTTTGCTTAACCTGAGGCGGCAACTGCCCCGGAATGTTC TGGACACCTCCTGGCCCACACCCCCACCTGCCCTGAGAGAGGCCTCAGAACTGCTACGGGAACTGTGCAT GAAGAACATGGTGTGGAAGTACTGCCGGAGCATCAGCCCTGAGTGGAAGCAGCAGCTGCAGCAAAAGGCG GTGGCTAGTGAAATTTTCAAGGGCAAGAAGGACAACTACCCCCAGAGTGTCCCCAGACTCTTCATTAGCA CACGGCTTGGCACAGAGGAGATCAGCCCCAGAGTGCTTCAATCCTTGGGCTCTGAACCCATCCAGTATGC CGTGCCCGTGGTAAAATACGACCGTAAGGGTTACAAGCCTCGCCCCCGGCAGCTGCTGCTCACGCCCAGT GCTGTGGTCATTGTGGAGGATGCTAAAGTCAAGCAGAGAATTGATTATGCCAACCTAACCGGAATCTCTG TCAGTAGCCTGAGTGATAGCCTATTTGTGCTTCACGTGCAGCGTGAAGACAACAAGCAGAAGGGAGATGT GGTGCTGCAGAGTGATCATGTGATCGAGACACTAACCAAGACGGCCCTCAGTGCTGACCGCGTGAACAAT ATCAACATCAACCAGGGCAGCATAACGTTTGCAGGGGGTCCAGGCAGGGACGGCATCATTGACTTCACAT CGGGCTCAGAGCTTCTCATCACCAAGGCTAAGAATGGCCACCTGGCTGTGGTGGCCCCACGGCTGAATTC TCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001080774
Insert Size	3087 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001080774.1, NP_001074243.1
RefSeq Size	5318 bp
RefSeq ORF	3087 bp
Locus ID	17913
UniProt ID	Q9WTI7
Gene Summary	This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The protein functions in intracellular vesicle transport to the plasma membrane. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 4. The resulting isoform (b) has a shorter N terminus when compared to isoform c. Variants 2 and 3 encode the same isoform.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.