Kif5a (NM_001039000) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D10Bwg0738e; Khc; Kif5; Kns; mKIAA4086
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229285 representing NM_001039000 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGACTAACAACGAATGCAGCATCAAGGTGCTTTGCCGATTTCGGCCCCTGAACCAGGCCGAGA TTCTGCGGGGGGACAAGTTCATCCCCATTTTCCAAGGGGACGACAGCGTCATTATTGGGGGAAAGCCATA TGTCTTTGACCGCGTCTTCCCCCCAAACACCACTCAGGAGCAGGTTTACCACGCCTGTGCCATGCAGATC GTCAAAGACGTCCTTGCTGGTTACAATGGCACAATCTTCGCTTATGGACAGACATCCTCAGGGAAAACGC ATACCATGGAGGGGAAGCTGCACGACCCTCAGCTGATGGGCATCATTCCCCGGATCGCTCGAGACATCTT CAACCACATCTACTCCATGGATGAGAACCTTGAATTCCACATTAAGGTATCTTACTTCGAGATTTACCTG GATAAGATCCGTGACCTTTTGGATGTGACCAAGACGAACCTGTCCGTGCATGAGGACAAAAACCGGGTGC CGTTTGTCAAGGGTTGTACCGAACGCTTTGTGTCCAGCCCAGAGGAGATTCTGGATGTGATCGATGAGGG GAAGTCCAACCGTCACGTAGCTGTCACCAACATGAACGAGCACAGTTCTCGGAGCCACAGCATCTTCCTC ATCAACATCAAGCAGGAGAACGTAGAGACCGAGCAGAAGCTCAGCGGGAAGCTGTACCTCGTGGATCTGG CCGGAAGCGAGAAGGTCAGCAAGACAGGGGCAGAGGGAGCCGTTCTGGACGAGGCAAAGAATATCAACAA GTCGCTGTCGGCCCTGGGGAACGTGATCTCTGCACTGGCAGAGGGCACCAAAAGCTACGTGCCGTACCGC GACAGCAAAATGACGAGGATTCTCCAGGACTCTCTGGGAGGGAACTGCAGGACTACCATGTTCATCTGCT GCTCGCCGTCCAGCTACAATGACGCAGAGACCAAGTCCACGCTCATGTTTGGACAGCGGGCGAAGACCAT CAAGAACACTGCCTCAGTGAATCTGGAGCTGACTGCTGAGCAGTGGAAGAAGAAGTATGAGAAGGAGAAG GAGAAGACCAAGGCCCAGAAGGAGACAATTGCGAAGCTAGAGGCTGAGCTTAGCCGGTGGCGCAATGGAG AGAATGTGCCTGAGACTGAGCGCCTGGCTGGAGAGGACTCAGCTCTGGGAGCTGAGCTCTGCGAGGAGAC CCCTGTGAATGACAACTCATCCATTGTGGTACGCATCGCACCTGAGGAAAGGCAGAAATATGAGGAAGAG ATCCGCCGTCTCTACAAGCAGCTTGATGACAAGGATGATGAGATCAACCAGCAGAGCCAGCTCATTGAGA AGCTGAAGCAGCAGATGCTGGACCAGGAAGAGCTGCTCGTGTCCACTCGGGGAGACAACGAGAAGGTCCA GCGGGAGCTTAGCCACCTGCAGTCCGAGAACGATGCTGCGAAGGACGAGGTGAAGGAAGTGCTGCAGGCC CTAGAGGAGCTGGCGGTCAACTACGACCAGAAGTCCCAGGAGGTGGAGGAGAAGAGCCAGCAGAACCAGC TGCTGGTGGACGAGCTGTCCCAGAAAGTGGCCACCATGCTGTCCCTGGAGTCCGAGCTACAGCGGCTCCA GGAGGTCAGTGGACACCAGCGAAAGCGGATCGCTGAGGTGCTGAATGGGCTGATGAGGGACCTGAGTGAG TTCAGTGTCATCGTGGGCAACGGCGAGATTAAGCTGCCCGTGGAGATCAGTGGGGCCATCGAGGAGGAGT TCACGGTGGCCCGGCTCTACATCAGCAAGATCAAGTCGGAGGTGAAGTCCGTGGTTAAGCGATGTCGGCA GCTGGAGAACCTCCAGGTGGAGTGTCATCGCAAGATGGAGGTGACCGGTAGGGAGCTGTCATCTTGCCAA CTGCTCATCTCACAGCATGAGGCCAAGATCCGTTCACTCACGGAGTACATGCAGACTGTGGAGTTGAAGA AACGGCACCTGGAAGAGTCCTACGACTCCCTGAGCGATGAGCTTGCCAGGCTCCAGGCGCACGAAACTGT ACACGAGGTAGCTCTGAAAGACAAGGAGCCAGACACACAGGACGCGGAGGAGGTGAAGAAGGCCCTGGAA CTACAGATGGAGAATCACCGTGAGGCCCATCACCGGCAGCTGGCCCGCCTCCGGGATGAGATTAATGAGA AACAGAAAACCATTGATGAGCTGAAAGACCTGAACCAGAAGCTCCAGTTAGAGCTGGAGAAGCTTCAGGC CGACTATGAGAGGCTGAAGAATGAAGAGAACGAGAAGAGCGCCAAGCTCCAGGAGCTGACATTTCTGTAT GAGCGACATGAGCAGTCCAAGCAGGACCTCAAGGGGCTGGAGGAGACAGTTGCCCGTGAACTCCAGACCC TCCACAACCTTCGCAAGCTGTTCGTTCAAGACGTCACGACTCGAGTCAAGAAAAGTGCAGAAATGGAGCC CGAGGACAGTGGGGGGATTCATTCCCAAAAGCAGAAGATCTCCTTTCTTGAGAACAACCTGGAACAGCTT ACAAAGGTTCACAAACAGCTGGTACGTGACAATGCAGATCTGCGTTGTGAGCTTCCTAAATTGGAAAAAC GACTTCGGGCTACGGCTGAGAGAGTTAAGGCCCTGGAGGGTGCACTGAAGGAGGCCAAGGAGGGCGCTAT GAAGGACAAGCGTAGATACCAGCAGGAGGTGGACCGCATCAAAGAAGCCGTGCGGTACAAGAGCTCCGGC AAGCGGGGCCATTCTGCCCAGATCGCTAAGCCTGTGAGGCCTGGCCACTATCCTGCCTCCTCACCCACCA ACCCCTACGGCACCCGGAGCCCCGAGTGTATCAGCTACACCAACAACCTCTTCCAGAACTACCAGAACCT GCACCTGCAGGCTGCGCCTAGCTCCACTTCAGATATGTACTTTGCCAGCAGCGGAGCCACATCTGTTGCC CCCTTGGCTTCCTACCAGAAGGCCAACATGGACAATGGAAATGCCACAGATATCAACGACAACAGGAGTG ACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTCCACCAAGAGACAGCAGCCAG CTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001039000
Insert Size	3084 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001039000.4, NP_001034089.1
RefSeq Size	6334 bp
RefSeq ORF	3084 bp
Locus ID	16572
UniProt ID	P33175
Gene Summary	Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL) (PubMed:12682084). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons (PubMed:21976701). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer and the predominant transcript. Variants 1 and 2 encode the same protein.

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