Ephb2 (NM_001290753) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cek5; Dr; Drt; Er; Erk; ETECK; Hek5; Nu; Nuk; Prk; Prkm5; Qek5; Sek; Sek3; Tyr; Tyro5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229249 representing NM_001290753 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTGCGCAGGCTGGGGGCCGCGCTGCTGCTGCTGCCGCTGCTAGCCGCCGTGGAAGAAACCCTGA TGGACTCTACGACAGCAACGGCTGAGCTGGGCTGGATGGTACATCCCCCATCAGGGTGGGAAGAGGTGAG CGGCTACGACGAGAACATGAACACTATCCGTACCTACCAGGTGTGCAATGTCTTTGAGTCAAGCCAGAAC AACTGGCTGCGGACCAAATTCATCCGGCGCCGCGGCGCCCACCGCATCCACGTGGAGATGAAGTTCTCGG TGCGTGACTGCAGCAGCATTCCCAGCGTGCCGGGCTCCTGCAAGGAGACCTTCAACCTCTACTACTATGA GGCTGATTTTGACTTAGCCACCAAAACCTTTCCCAACTGGATGGAGAATCCGTGGGTGAAGGTGGACACC ATCGCGGCCGATGAGAGCTTCTCTCAGGTGGACCTGGGTGGCCGCGTCATGAAAATCAACACTGAGGTGC GAAGCTTCGGTCCCGTGTCCCGCAATGGTTTCTACCTGGCCTTCCAGGACTATGGCGGCTGTATGTCCCT CATTGCTGTGCGCGTCTTCTACCGGAAGTGCCCCCGAATCATCCAGAATGGTGCCATCTTCCAGGAGACA CTGTCGGGGGCTGAGAGCACTTCGCTGGTGGCAGCTCGGGGCAGCTGCATCGCCAATGCTGAAGAAGTGG ATGTGCCCATCAAACTCTACTGTAACGGGGACGGCGAATGGCTGGTGCCCATAGGTCGCTGCATGTGCAA GGCGGGCTTCGAGGCTGTGGAGAACGGCACCGTCTGCCGAGGTTGTCCATCAGGAACCTTCAAGGCCAAC CAAGGGGACGAAGCCTGCACCCACTGTCCCATCAACAGCCGCACCACCTCCGAGGGTGCCACCAACTGTG TATGCCGCAACGGCTACTACAGGGCCGACCTGGACCCCTTAGACATGCCTTGCACAACCATCCCCTCTGC GCCCCAGGCTGTGATCTCCAGCGTCAACGAGACGTCCCTCATGCTAGAGTGGACCCCACCCCGAGACTCG GGGGGTCGCGAGGATCTTGTTTACAACATCATCTGCAAGAGCTGTGGCTCCGGCCGGGGCGCATGCACGC GCTGCGGGGACAACGTGCAGTACGCGCCCCGCCAGCTGGGCCTGACTGAGCCGCGCATCTACATCAGTGA CCTGCTGGCACACACGCAGTACACCTTCGAGATCCAGGCCGTGAACGGTGTGACTGACCAGAGTCCCTTC TCACCTCAGTTCGCCTCTGTGAACATCACCACCAACCAAGCAGCACCATCGGCCGTGTCCATCATGCACC AGGTGAGCCGCACTGTGGACAGCATCACCCTGTCGTGGTCCCAGCCAGACCAGCCCAACGGTGTGATCCT GGACTACGAGCTGCAGTACTATGAGAAGCAGGAGCTCAGTGAGTACAACGCCACGGCCATAAAAAGCCCC ACCAACACAGTCACTGTGCAGGGCCTCAAAGCCGGCGCCATCTATGTCTTCCAGGTGCGGGCACGCACCG TTGCAGGCTATGGGCGCTACAGTGGCAAGATGTACTTCCAAACCATGACAGAAGCCGAGTACCAGACCAG CATCAAGGAAAAGCTACCCCTCATCGTTGGCTCCTCCGCCGCCGGCTTAGTCTTCCTCATCGCTGTGGTC GTCATTGCCATCGTATGTAACAGACGGGGGTTTGAGCGTGCCGACTCAGAGTACACGGACAAGCTACAAC ACTACACCAGCGGACACATGACCCCAGGCATGAAGATCTATATAGACCCTTTCACCTATGAAGATCCTAA TGAGGCAGTGCGGGAGTTTGCCAAGGAAATTGACATCTCCTGTGTCAAGATTGAGCAGGTGATCGGAGCA GGGGAATTTGGTGAGGTCTGCAGTGGCCATTTGAAGCTGCCAGGCAAGAGAGAGATCTTTGTAGCCATCA AGACCCTCAAGTCAGGATACACGGAGAAACAGCGCCGGGACTTCCTGAGTGAGGCATCCATCATGGGCCA GTTCGACCACCCCAATGTCATCCATCTGGAAGGGGTTGTCACCAAGAGCACACCTGTCATGATCATCACT GAATTCATGGAGAACGGATCTCTGGACTCCTTCCTCCGGCAAAACGATGGGCAGTTCACAGTCATCCAAC TGGTGGGCATGCTGAGGGGCATTGCAGCCGGCATGAAGTACCTGGCGGACATGAACTACGTGCACCGTGA CCTTGCTGCTCGAAACATCCTCGTCAACAGCAACCTGGTGTGTAAGGTGTCTGATTTTGGGCTCTCACGC TTCCTGGAGGATGACACGTCTGACCCCACCTATACCAGCGCTCTGGGTGGGAAGATCCCCATCCGTTGGA CGGCACCGGAAGCCATCCAGTACCGGAAATTCACCTCGGCCAGTGATGTGTGGAGCTATGGCATCGTCAT GTGGGAGGTGATGTCCTACGGGGAACGACCCTACTGGGACATGACCAATCAAGACGTAATCAACGCCATT GAACAGGACTACAGACTACCTCCGCCCATGGACTGCCCTAGTGCCCTGCACCAGCTCATGCTGGACTGCT GGCAGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAGATTGTCAACACGCTGGACAAGATGATCCGAAA CCCCAACAGCCTCAAAGCCATGGCACCCCTGTCCTCTGGCATCAACCTGCCACTGCTGGACCGCACGATA CCGGACTACACCAGCTTTAACACGGTGGATGAGTGGCTAGAGGCCATCAAGATGGGCCAGTACAAGGAGA GCTTTGCCAACGCCGGCTTTACCTCTTTCGACGTTGTATCTCAGATGATGATGGAGGACATTCTCCGCGT TGGGGTCACTCTAGCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTGATGCGGGCCCAGATGAAC CAGATCCAGTCTGTAGAGGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290753
Insert Size	2964 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290753.2, NP_001277682.1
RefSeq Size	10929 bp
RefSeq ORF	2964 bp
Locus ID	13844
UniProt ID	P54763
Gene Summary	This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors consist of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. The encoded receptor preferentially binds membrane-bound ephrin-B ligands and is involved in nervous system and vascular development. This gene is used as a marker of intestinal stem cells. Homozygous knockout mice for this gene exhibit impaired axon guidance and vestibular function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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