Trim24 (NM_001272076) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A130082H20Rik; AI447469; D430004I05Rik; Tif; TIF1; TIF1-alpha; Tif1a; TIF1alpha
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229247 representing NM_001272076 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGATATTTTTGATTACACTTTTATCGGTGGGTGTGGAGATGCTTGTCCCACATGCGCATGTGGAA GTCAGAGGATAACTTGGAAAAGTCAGTTCTTTCCATCCTGTGTTCGTCCCATGGATAAAACTGCTGTCAC TGGTGATGAGCAAGTTGGAGTCATTCGATGCCCAGTTTGCAGTCAAGAGTGTGCTGAGAGACACATCATA GACAACTTTTTTGTGAAGGACACCACTGAAGTTCCTAGTAGTACAGTAGAAAAGTCTAATCAGGTATGTA CAAGCTGTGAAGACAATGCAGAAGCTAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACATG TATTAGAGCTCACCAGAGGGTGAAGTTCACAAAAGACCACACAGTCAGGCAGAAAGAAGAAGTATCTCCA GAGGCAGTTGGGGTGACCAGTCAGCGACCAGTGTTTTGTCCCTTCCATAAAAAGGAGCAGTTGAAACTTT ACTGTGAAACATGTGATAAACTGACCTGTCGAGACTGCCAGCTGCTAGAACACAAAGAACACAGGTATCA ATTTATAGAAGAAGCTTTTCAGAATCAAAAAGTGATCATAGATACTCTAATCACCAAACTGATGGAAAAA ACAAAATATATAAAGTATACAGGAAATCAGATCCAAAATAGGATAATTGAAATAAATCAAAACCAAAAGC AGGTGGAACAGGATATTAAAGTTGCCATCTTCACATTGATGGTGGAGATAAACAAAAAAGGGAAAGCTCT GCTGCACCAGCTTGAGAGTCTTGCAAAGGACCATCGAATGAAACTCATGCAACAACAGCAGGAAGTGGCT GGGCTTTCTAAGCAGTTAGAGCACGTCATGCATTTTTCTAAATGGGCTGTTTCCAGTGGCAGCAGCACAG CCTTGCTGTACAGCAAGCGGCTGATTACATACAGGTTACGGCACCTTCTTCGTGCAAGGTGTGATGCTTC TCCTGTGACCAACACCACCATCCAGTTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATTATCAACTTG GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAGCAGA GTTCACAGCCACCAGGTGGTTTACCTTCCAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA GTTACGACTCCAGCATATTCAGCAACAGGTAATGGCTCAGAGGCAACAGGTGCAACGGAGGCCAGCACCT GTGGGTTTACCAAACCCTAGAATGCAGGGGCCCATCCAGCAGCCTTCCATCTCTCATCAGCATCCGCCAC CACGCTTAATAAACTTTCAGAATCACAGCCCTAAGCCCAATGGACCAGTTCTTCCTCCTTATCCTCAGCA GCTGAGATATTCACCAAGCCAGAATGTACCTCGGCAGACAACAATAAAGCCCAACCCCTTGCAAATGGCT TTTTTGGCTCAACAGGCCATAAAACAGTGGCAGATCAGCAGTGTACAGGCTCCGCCCACAACTGCCAGCA GCTCCTCCTCCACGCCGTCCAGCCCCACAATCACAAGTGCAGCTGGGTACGATGGAAAAGCTTTTAGTTC ACCCATGATTGATCTGAGTGCACCGGTGGGAGGGTCTTACAATCTTCCTTCTCTTCCAGATATTGATTGT TCAAGTACTATAATGTTGGACAACATTGCAAGGAAAGACACAGGTGTAGATCACGCCCAGCCGAGGCCTC CGTCAAACAGAACGGTGCAGTCACCAAATTCATCAGTGCCATCTCCAGGCCTTGCAGGGCCTGTTACTAT GACTAGCGTCCATCCCCCAATACGTTCACCTAGTGCCTCCAGTGTTGGAAGTCGAGGAAGCTCTGGCTCT TCCAGCAAACCAGCAGGAGCTGATTCTACTCACAAGGTCCCAGTAGTCATGCTGGAGCCAATTCGAATAA AACAGGAAAACAGTGGACCACCTGAAAATTATGATTTTCCTGTTGTTATAGTAAAACAAGAATCAGATGA AGAATCTAGACCTCAAAATACTAACTATCCAAGAAGCATACTTACCTCCCTCCTCTTAAACAGCAGTCAG AGCTCTGCTTCTGAGGAAACCGTGTTACGATCTGATGCCCCTGATAGTACAGGAGATCAGCCTGGACTCC ATCAAGAAAATTCCTCAAATGGAAAGTCTGAGTGGTCGGATGCCTCCCAGAAGTCCCCTGTGCATGTCGG AGAGACGAGGAAGGAGGATGACCCCAATGAAGACTGGTGTGCTGTTTGTCAAAATGGTGGGGAACTCCTA TGCTGTGAGAAATGTCCTAAAGTATTCCATCTTACTTGTCATGTGCCCACCTTGACAAATTTTCCAAGTG GAGAATGGATCTGTACTTTCTGCCGAGACTTATCTAAGCCAGAGGTTGACTATGATTGTGATGTTCCCAG TCACCACTCAGAGAAACGGAAAAGTGAAGGCCTTACTAAGTTAACGCCAATAGACAAAAGGAAATGTGAA CGCCTACTTCTGTTTCTTTACTGCCATGAAATGAGCCTGGCTTTCCAAGACCCTGTTCCTCTAACTGTGC CTGATTATTATAAAATAATTAAAAACCCAATGGACTTGTCAACCATCAAGAAAAGACTTCAGGAGGATTA TTGCATGTATACAAAGCCTGAAGACTTTGTAGCTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTC AATGAGCCTGATTCTGAAGTAGCCAATGCTGGTATAAAACTTGAAAGCTATTTTGAAGAACTTCTAAAGA ATCTTTATCCAGAAAAAAGGTTTCCTAAGGTAGAATTCAGGCATGAAGCAGAAGACTGTAAGTTCAGTGA CGACTCAGACGATGACTTTGTACAGCCCCGGAAGAAGCGTCTCAAGAGCACCGAGGATCGCCAGCTGCTT AAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001272076
Insert Size	2946 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001272076.1, NP_001259005.1
RefSeq Size	5771 bp
RefSeq ORF	2946 bp
Locus ID	21848
UniProt ID	Q64127
Gene Summary	The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which are typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. This protein, which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin, has important roles in differentiation, development, and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene, which results in the fusion protein T18, has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct and shorter N-terminus that lacks the N-terminal RING domain, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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