Opa1 (NM_001199177) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200011N24Rik; AI225888; AI847218; lilr3; mKIAA0567
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229246 representing NM_001199177 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGAGCAGGTCGGGCGGCCGTGGCCTGTGAAGTCTGCCAATCCTTAGTGAAACACAGTTCTGGAA TACAAAGAAACGTACCGCTCCAAAAACTCCATCTGGTTTCACGAAGTATTTATCGTTCACATCATCCTGC CCTCAAGCTTCAAAGACCCCAACTAAGGACACCATTTCAGCAGTTCTCTTCTCTAACTCACCTTTCATTA CATAAATTGAAACTTTCTCCAATTAAATATGGCTACCAGCCCCGCAGGAACTTTTGGCCAGCAAGGTTAG CTGCAAGACTCTTAAAACTTCGATATATCATACTGGGATCTGCTGTTGGAGGTGGCTATACAGCCAAAAA AACCTTCGATGAATGGAAAGATATGATACCAGACCTTAGTGACTATAAGTGGATTGTGCCTGACTTTATA TGGGAAATTGATGAGTATATTGATTTGGAGAAAATTAGAAAAGCCCTGCCCAGCTCAGAAGACCTTGCCA GTTTAGCTCCCGACCTGGACAAGATTACTGAGAGCCTCAGCTTGTTGAAGGACTTCTTCACTGCAGGTCC CAAATTGGTTAGTGAAGTCCTAGAAGTTTCTGAGGCCCTTCTCTTGTTAGGTTCACCTGGAGAAACAGCA TTTCGAGCAACAGATCATGGATCTGAAAGTGACAAGCATTACAGGAAGGTGTCAGACAAAGAAAAGATTG ACCAACTTCAAGAAGAACTTCTGCATACTCAGTTAAAGTATCAGAGGATCTTGGAGCGCCTGGAAAAGGA GAACAAAGAGCTGCGGAAGCTGGTGCTGCAGAAGGACGACAAAGGCATCCACCACAGGAAGCTCAAGAAA TCTTTGATTGATATGTATTCTGAAGTTCTTGATGTTCTTTCTGATTATGATGCCAGTTACAATACACAAG ATCACCTACCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGGAAAACCAGTGTGCTGGAAATGATTGC TCAGGCCCGGATCTTCCCGAGAGGGTCCGGCGAGATGATGACACGCTCTCCAGTGAAGGTGACTCTCAGT GAAGGCCCTCACCATGTGGCCTTGTTTAAAGATAGCTCTCGGGAATTTGATCTCACCAAGGAGGAAGATC TTGCAGCATTAAGACATGAAATCGAACTCCGAATGAGGAAAAATGTGAAAGAAGGTTGTACTGTTAGCCC CGAGACCATATCTCTAAATGTCAAAGGCCCTGGGCTGCAGAGGATGGTGCTCGTGGACTTGCCTGGTGTC ATCAACACCGTGACATCAGGCATGGCTCCCGACACAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACA TGCAGAATCCTAACGCCATCATCCTGTGCATCCAAGACGGATCCGTAGATGCTGAGCGCAGTATTGTTAC AGACTTGGTCAGTCAAATGGATCCTCATGGAAGAAGAACCATATTTGTTTTGACCAAAGTAGACCTGGCA GAAAAAAATGTAGCCAGTCCAAGCAGGATACAACAGATAATTGAAGGCAAGCTCTTCCCAATGAAAGCTC TGGGTTATTTTGCTGTCGTAACAGGAAAAGGAAACAGCTCTGAAAGTATTGAAGCTATAAGAGAGTATGA AGAGGAATTTTTTCAGAATTCAAAACTGCTAAAGACAAGCATGCTAAAGGCACACCAGGTCACCACGAGA AATCTCAGCCTTGCTGTGTCAGACTGCTTTTGGAAAATGGTTCGAGAGTCAGTTGAACAACAGGCTGATA GTTTTAAAGCCACGCGCTTTAACCTAGAGACGGAATGGAAGAATAACTACCCGCGCCTGCGAGAGCTCGA CAGGAATGAACTCTTTGAAAAAGCTAAAAATGAGATCCTCGATGAGGTCATCAGTCTGAGCCAGGTCACG CCAAAGCACTGGGAGGAAATCCTGCAGCAATCCCTGTGGGAACGAGTGTCAACACATGTGATTGAGAACA TCTACCTTCCAGCTGCCCAGACCATGAATTCGGGAACATTTAACACCACAGTAGACATCAAGCTTAAACA GTGGACTGACAAGCAGCTTCCTAATAAAGCAGTCGAGGTTGCCTGGGAGACTCTACAAGAGGAATTTTCC CGCTTCATGACAGAACCCAAAGGAAAGGAACACGACGACATATTTGACAAACTTAAGGAGGCTGTGAAGG AGGAGAGTATCAAGCGGCACAAGTGGAACGACTTTGCCGAGGATAGCTTGAGGGTTATTCAGCACAATGC TTTGGAAGACCGGTCCATATCAGATAAGCAACAGTGGGATGCAGCCATTTACTTCATGGAAGAGGCGCTT CAAGGTCGTCTCAAGGATACTGAAAATGCTATTGAAAACATGATTGGGCCAGACTGGAAAAAGAGGTGGA TGTACTGGAAGAATCGGACCCAAGAGCAGTGTGTTCACAACGAAACCAAGAACGAGTTGGAGAAGATGCT GAAGGTTAATGATGAGCACCCAGCTTACCTGGCAAGTGATGAGATTACCACAGTCCGGAAGAACCTGGAG TCTCGAGGAGTGGAAGTCGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTCT TAAAAACAGCTCTAAATCATTGTAACCTTTGTCGCAGAGGTTTTTATTACTACCAGAGGCATTTTATAGA TTCTGAGCTGGAATGCAATGACGTGGTCCTGTTTTGGCGAATACAGCGCATGCTCGCTATCACTGCCAAT ACATTAAGGCAGCAGCTTACAAACACTGAAGTTAGGCGACTAGAGAAAAACGTTAAAGAGGTATTAGAAG ATTTTGCAGAAGACGGTGAGAAGAAGGTTAAATTGCTCACTGGCAAACGAGTTCAGCTGGCAGAAGATCT CAAGAAAGTTAGAGAAATTCAAGAAAAGCTTGATGCTTTCATTGAAGCTCTTCACCAGGAGAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001199177
Insert Size	2937 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199177.1, NP_001186106.1
RefSeq Size	6002 bp
RefSeq ORF	2937 bp
Locus ID	74143
Gene Summary	Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:11847212, PubMed:24616225, PubMed:26785494, PubMed:28746876). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (By similarity). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (PubMed:16839884, PubMed:16839885). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (PubMed:16839884, PubMed:16839885). Plays a role in mitochondrial genome maintenance (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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