Chrd (NM_001278041) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Chd
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229235 representing NM_001278041 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGCCTCCCGGCCCCGCCGGCCCCGCGGCTGCTCCTCGGGCTGCTGCTGCTCGGCTCGCGGCCGG CCAGTGGCACTGGCCCTGAGCCCCCAGCACTGCCCATCCGTTCCGAGAAGGAGCCGCTGCCTGTCCGGGG AGCGGCAGAGCCCGGAGCAGGCATTACGGCACGAGCAACCGGACAGCGGCCGCCAGCCAAGCCCGTCCCC GCAGGCTGCTCCTTCGGCGGGAAGGTCTATGCCTTGGACGAGACGTGGCACCCGGACCTGGGGGAGCCTT TTGGGGTGATGCGCTGCGTGCTGTGCGCCTGTGAAGCGCCTCAGTGGGCTCGCCGTGGGAGGGGTCCTGG CAGGGTCAGCTGCAAGAACATCAAACCTCAGTGCCCCACCCTGGCCTGCAGGCAGCCGCGCCAGCTGCCA GGACACTGCTGCCAGACCTGCCCGCAGGAGCGCAGCAATCTAGATCCACAGCCCGCTGGCCTGGTCTTCG AGTATCCAAGGGATCCAGAGCATCGCAGTTACAGTGATCGAGGGGAACCCGGCGTTGGGGAGCGGACCCG TGCTGATGGCCACACAGACTTTGTGGCGCTGCTGACAGGACCGAGGTCGCAGGCGGTAGCTCGTGCTCGA GTCTCTTTGCTGCGCTCAAGTTTACGCTTCTCTGTCTCCTACCAGCGGCTGGACCGTCCCAGCAGGGTTC GTTTCACAGATCCCACAGGCAACATCCTGTTTGAACACCCTGCAACCCCCACCCAGGATGGCCTGGTCTG TGGGGTGTGGCGGGCAGTGCCTCGGCTGTCTGTGAGGCTCCTGAGGGCAGAGCAGCTTCGGGTAGCCCTT GTGACATCCACTCACCCTTCAGGAGAAGTCTGGGGGCCTCTCATTTGGCAGGGTGCTCTCGCTGCAGAGA CCTTCAGTGCCATCCTGACCCTGGAAGACCCACTGCAGCGGGGTGTGGGGGGCATAGCCCTGCTCACCCT CAGTGACACAGAAGATTCCTTGCATTTTTTGCTGCTCTTCCGGGGTCTGCTGGGAGGACTAGCTCAGGCT CCCTTGAAGCTTCAGATTCTCCACCAGGGACAGCTACTTCGGGAGCTCCAGGCCAACACCTCCGCTCAGG AGCCAGGTTTTGCTGAGGTGCTGCCCAGCCTTACAGACCAAGAGATGGACTGGTTGGAGCTGGGGGAGCT GCAGATGGTCCTAGAGAAGGCGGGTGGGCCAGAGCTACGCATCAGTGGATACATCACCACCAGGCAGAGC TGTGATGTCCTTCAAAGTGTCCTTTGTGGGGCTGATGCCCTGATCCCAGTCCAGACGGGTGCTGCTGGGT CAGCCAGCTTCATATTGCTAGGAAATGGCTCCCTTATCTATCAGGTGCAAGTGGTAGGTACAGGTAGCGA GGTGGTGGCCATGACACTGGAGACCAAGCCTCAGCGGAAGAACCAGCGCACTGTCCTGTGCCACATGGCT GGACTCCAGCCGGGAGGACACATGGCTGTGGGTATGTGCTCTGGGCTGGGTGCCCGAGGGGCTCATATGC TGCTACAGAACGAGCTGTTCCTGAATGTTGGTACCAAGGACTTCCCAGATGGAGAGCTTCGGGGGCATGT GACTGCCCTATGCTACAGTGGGCACAGTGCCCGCTATGACAGATTGCCTGTGCCTCTGGCAGGGGCACTA GTGCTGCCCCCTGTGCGGAGTCAGGCAGCAGGGCACGCCTGGCTCTCCTTGGATACACATTGTCACTTAC ACTATGAAGTTCTATTGGCTGGGCTTGGTGGCTCAGAGCAAGGAACTGTCACTGCCCACCTCCTTGGGCC TCCTGGGATGCCAGGGCCCCAGCGGCTGCTGAAGGGATTCTATGGCTCAGAGGCTCAGGGCGTGGTAAAA GATCTGGAGCCTGTGCTGCTGCGGCACCTGGCACAGGGCACTGCCTCCCTGCTCATCACCACCAAGAGTA GCCCCAGAGGAGAACTACGTGGGCAGGTGCACATTGCCAGTCAGTGTGAGGCGGGAGGCCTGCGTCTGGC CTCAGAAGGAGTGCAGATGCCCTTGGCTCCAAATGGAGAGGCGGCTACGTCACCCATGCTGCCTGCTGGC CCTGGCCCTGAAGCCCCAGTACCAGCCAAACATGGCAGCCCTGGGAGACCCCGAGATCCTAACACATGTT TCTTCGAGGGGCAGCAGCGGCCCCACGGGGCTCGCTGGGCACCCAACTATGACCCACTCTGCTCCCTCTG CATCTGCCAGAGACGAACAGTGATCTGTGACCCTGTAGTATGCCCACCACCAAGCTGTCCCCACCCGGTG CAGGCACTGGACCAGTGCTGTCCCGTGTGTCCAGAGAAACAACGCAGTAGAGACCTCCCCAGCCTACCAA ATCTGGAGCCAGGAGAGGGCTGCTATTTTGATGGTGACCGGAGCTGGAGGGCAGCGGGTACCCGATGGCA CCCTGTTGTGCCCCCCTTTGGCCTAATTAAGTGTGCTGTCTGCACCTGCAAGGGGGCCACGGGAGAGGTG CACTGTGAGAAGGTGCAGTGTCCTCGCCTGGCCTGTGCTCAGCCTGTCCGTGCCAACCCCACCGACTGCT GCAAACAGTGTCCAGTAGGGTCAGGGACTAATGCCAAGCTGGGAGACCCCATGCAGGCTGATGGGCCTCG GGGGTGTCGCTTTGCTGGGCAGTGGTTCCCAGAGAATCAGAGCTGGCACCCATCAGTGCCCCCCTTTGGG GAGATGAGCTGTATTACCTGCAGATGTGGGGCAGGGGTACCCCACTGTGAGCGGGACGATTGTTCACCGC CGCTATCCTGTGGCTCAGGGAAGGAGAGTCGATGCTGTTCCCACTGCACAGCCCAAAGGTCCTCTGAGAC TAGAACCCTTCCAGAGCTGGAGAAAGAAGCGGAGCACTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001278041
Insert Size	2913 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278041.1, NP_001264970.1
RefSeq Size	3358 bp
RefSeq ORF	2913 bp
Locus ID	12667
UniProt ID	Q9Z0E2
Gene Summary	Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the 5' coding region compared to variant 1, which results in a longer isoform (2) containing an internal protein segment not found in isoform 1.

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