Usp15 (NM_001301628) Mouse Untagged Clone

CAT#: MC229208

Usp15 (untagged) - Mouse ubiquitin specific peptidase 15 (Usp15), transcript variant 2



  "NM_001301628" in other vectors (1)

CNY 9,220.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4921514G19Rik; AI327321; E430033I05Rik; Gcap; Gcap18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229208 representing NM_001301628
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAGGCGGAGCGGCGGACCTGGACACCCAGCGTTCTGACATCGCGACGCTGCTCAAAACCTCGC
TCCGGAAAGGGGACACCTGGTATCTAGTAGATAGTCGGTGGTTCAAACAGTGGAAAAAATATGTTGGCTT
TGACAGCTGGGACAAATACCAGATGGGAGATCAAAATGTCTATCCTGGACCCATCGATAACTCTGGACTT
CTCAAAGATGGCGACGCGCAGTCACTTAAGGAGCACCTTATTGATGAGTTGGATTACATACTCTTGCCAA
CTGAGGGCTGGAATAAACTTGTCAGCTGGTACACACTGATGGAGGGCCAGGAGCCAATAGCAAGAAAGGT
GGTAGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTCTACCTCACAGAACTGAAGCTCTGTGAG
AACGGGAACATGAACAATGTTGTAACTCGGAGGTTTAGCAAAGCTGACACAATAGATACGATTGAGAAGG
AAATAAGAAAAATCTTCAATATTCCAGATGAGAAGGAGGCCAGACTGTGGAACAAGTATATGAGCAACAC
ATTTGAACCACTGAATAAGCCAGACAGCACCATCCAGGATGCCGGCCTATACCAAGGACAGGTGTTAGTG
ATAGAGCAAAAAAATGAAGATGGAACGTGGCCCAGGGGTCCCTCTACTCCTAATGTGAAAAACTCAAATT
ATTGTCTCCCGTCATATACTGCTTATAAGAACTATGATTATTCAGAACCTGGAAGAAACAATGAACAGCC
AGGCCTGTGTGGCTTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCCATTCAGTGTTTGAGCAAC
ACACCTCCACTTACTGAATATTTCCTCAATGATAAGTACCAAGAAGAGCTGAATTTTGACAATCCCTTAG
GAATGAGAGGTGAAATAGCTAAATCATACGCTGAACTCATCAAGCAGATGTGGTCTGGAAAGTTTAGCTA
CGTCACTCCAAGAGCATTCAAGACACAGGTAGGGCGCTTCGCACCTCAGTTCTCTGGATATCAACAGCAG
GACTGCCAAGAACTACTGGCTTTCCTGTTGGATGGACTGCACGAGGACCTGAATAGAATTAGGAAAAAAC
CCTATATCCAGTTAAAAGATGCTGATGGCAGGCCAGACAAGGTGGTTGCTGAAGAAGCCTGGGAGAACCA
TTTAAAGCGAAATGATTCCATCATAGTAGACATATTCCATGGCCTTTTCAAGTCTACGTTAGTGTGTCCT
GAGTGTGCTAAGATCTCGGTGACCTTTGACCCTTTCTGTTACTTGACTCTTCCATTGCCTATGAAAAAAG
AACGTAGCTTGGAAGTTTATTTAGTTAGAATGGATCCACTTGCTAAACCTATGCAATACAAAGTTATTGT
GCCCAAAATTGGAAATATACTAGATCTTTGTACAGCGCTGTCTGCTTTGTCGGGAGTACCTGCAGATAAG
ATGATAGTCACTGACATATACAATCATAGATTTCACAGGATATTTGCCGTGGATGAAAACCTGAGTAGTA
TTATGGAGCGGGATGATATTTACGTGTTTGAAATTAACATCAACAGGGCAGAAGACACAGAGCATGTAGT
TATCCCTGTCTGCCTAAGAGAAAAATTCAGACACTCAAGTTACACTCACCACACTGGCTCTTCACTGTTC
GGCCAGCCTTTTCTTATGGCTATACCACGAAACAATACTGAAGATAAACTCTATAATCTTCTACTCTTGA
GAATGTGCCGGTATGTCAAAATGTCTACTGAAACTGAGGAAACAGATGGACACCTACGTTGCTGTGAGGA
CCAGAATATTAATGGGAATGGGCCAAATGGCCTACATGAAGAGGGTTCACCAAGTGAGATGGAAACCGAC
GAGCCAGATGACGAGTCCAGCCAGGATCAAGAGCTTCCCTCAGAGAACGAGAACAGTCAGTCTGAAGATT
CAGTCGGAGGAGATAACGATTCTGAAAATGGATTGTGCACTGAAGAAACATGCAAAGGTCAACTTACGGG
ACACAAAAAGCGCTTGTTTACATTCCAGTTCAACAACTTAGGCAACAATGACATCAACTACATCAAAGAC
GACACCAGTCACATCCGATTCGATGACAGGCAGCTTCGGCTGGACGAAAGATCTTTTCTCGCTTTGGACT
GGGACCCTGACTTGAAGAAGAGATACTTTGATGAGAATGCTGCAGAGGATTTTGAAAAACATGAAAGTGT
GGAATACAAACCTCCAAAAAGGCCCTTTGTGAAGTTAAAAGACTGCATTGAGCTCTTCACCACCAAGGAG
AAGCTGGGTGCTGAGGACCCCTGGTATTGTCCAAATTGTAAAGAGCATCAGCAAGCCACAAAGAAATTAG
ATCTGTGGTCCCTGCCTCCAGTTCTTGTGGTGCATCTCAAGAGATTCTCCTATAGTCGCTACATGAGAGA
CAAGCTAGATACATTAGTCGATTTTCCCATCAGTGACTTGGATATGTCAGAATTCCTAATTAACCCAAAT
GCAGGTCCTTGCCGCTATAATTTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACATTATA
CTGCTTTTGCAAAAAACAAAGATGATGGGAAGTGGTACTATTTCGATGACAGTAGCGTCTCCACTGCATC
TGAAGACCAAATCGTGTCCAAGGCAGCGTACGTGCTCTTCTACCAGAGACAGGACACTTTCAGTGGGACT
GGCTTCTTCCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACAGGTATCCCCCTGGAAAGTGACG
AAGACAGCAATGATAATGACAATGACCTAGAAAATGAAAACTGTATGCACACTAATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001301628
Insert Size 2859 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001301628.1, NP_001288557.1
RefSeq Size 11477 bp
RefSeq ORF 2859 bp
Locus ID 14479
UniProt ID Q8R5H1
Gene Summary The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms.[provided by RefSeq, Aug 2014]
Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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