Usp15 (NM_001301628) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921514G19Rik; AI327321; E430033I05Rik; Gcap; Gcap18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229208 representing NM_001301628 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGGCGGAGCGGCGGACCTGGACACCCAGCGTTCTGACATCGCGACGCTGCTCAAAACCTCGC TCCGGAAAGGGGACACCTGGTATCTAGTAGATAGTCGGTGGTTCAAACAGTGGAAAAAATATGTTGGCTT TGACAGCTGGGACAAATACCAGATGGGAGATCAAAATGTCTATCCTGGACCCATCGATAACTCTGGACTT CTCAAAGATGGCGACGCGCAGTCACTTAAGGAGCACCTTATTGATGAGTTGGATTACATACTCTTGCCAA CTGAGGGCTGGAATAAACTTGTCAGCTGGTACACACTGATGGAGGGCCAGGAGCCAATAGCAAGAAAGGT GGTAGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTCTACCTCACAGAACTGAAGCTCTGTGAG AACGGGAACATGAACAATGTTGTAACTCGGAGGTTTAGCAAAGCTGACACAATAGATACGATTGAGAAGG AAATAAGAAAAATCTTCAATATTCCAGATGAGAAGGAGGCCAGACTGTGGAACAAGTATATGAGCAACAC ATTTGAACCACTGAATAAGCCAGACAGCACCATCCAGGATGCCGGCCTATACCAAGGACAGGTGTTAGTG ATAGAGCAAAAAAATGAAGATGGAACGTGGCCCAGGGGTCCCTCTACTCCTAATGTGAAAAACTCAAATT ATTGTCTCCCGTCATATACTGCTTATAAGAACTATGATTATTCAGAACCTGGAAGAAACAATGAACAGCC AGGCCTGTGTGGCTTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCCATTCAGTGTTTGAGCAAC ACACCTCCACTTACTGAATATTTCCTCAATGATAAGTACCAAGAAGAGCTGAATTTTGACAATCCCTTAG GAATGAGAGGTGAAATAGCTAAATCATACGCTGAACTCATCAAGCAGATGTGGTCTGGAAAGTTTAGCTA CGTCACTCCAAGAGCATTCAAGACACAGGTAGGGCGCTTCGCACCTCAGTTCTCTGGATATCAACAGCAG GACTGCCAAGAACTACTGGCTTTCCTGTTGGATGGACTGCACGAGGACCTGAATAGAATTAGGAAAAAAC CCTATATCCAGTTAAAAGATGCTGATGGCAGGCCAGACAAGGTGGTTGCTGAAGAAGCCTGGGAGAACCA TTTAAAGCGAAATGATTCCATCATAGTAGACATATTCCATGGCCTTTTCAAGTCTACGTTAGTGTGTCCT GAGTGTGCTAAGATCTCGGTGACCTTTGACCCTTTCTGTTACTTGACTCTTCCATTGCCTATGAAAAAAG AACGTAGCTTGGAAGTTTATTTAGTTAGAATGGATCCACTTGCTAAACCTATGCAATACAAAGTTATTGT GCCCAAAATTGGAAATATACTAGATCTTTGTACAGCGCTGTCTGCTTTGTCGGGAGTACCTGCAGATAAG ATGATAGTCACTGACATATACAATCATAGATTTCACAGGATATTTGCCGTGGATGAAAACCTGAGTAGTA TTATGGAGCGGGATGATATTTACGTGTTTGAAATTAACATCAACAGGGCAGAAGACACAGAGCATGTAGT TATCCCTGTCTGCCTAAGAGAAAAATTCAGACACTCAAGTTACACTCACCACACTGGCTCTTCACTGTTC GGCCAGCCTTTTCTTATGGCTATACCACGAAACAATACTGAAGATAAACTCTATAATCTTCTACTCTTGA GAATGTGCCGGTATGTCAAAATGTCTACTGAAACTGAGGAAACAGATGGACACCTACGTTGCTGTGAGGA CCAGAATATTAATGGGAATGGGCCAAATGGCCTACATGAAGAGGGTTCACCAAGTGAGATGGAAACCGAC GAGCCAGATGACGAGTCCAGCCAGGATCAAGAGCTTCCCTCAGAGAACGAGAACAGTCAGTCTGAAGATT CAGTCGGAGGAGATAACGATTCTGAAAATGGATTGTGCACTGAAGAAACATGCAAAGGTCAACTTACGGG ACACAAAAAGCGCTTGTTTACATTCCAGTTCAACAACTTAGGCAACAATGACATCAACTACATCAAAGAC GACACCAGTCACATCCGATTCGATGACAGGCAGCTTCGGCTGGACGAAAGATCTTTTCTCGCTTTGGACT GGGACCCTGACTTGAAGAAGAGATACTTTGATGAGAATGCTGCAGAGGATTTTGAAAAACATGAAAGTGT GGAATACAAACCTCCAAAAAGGCCCTTTGTGAAGTTAAAAGACTGCATTGAGCTCTTCACCACCAAGGAG AAGCTGGGTGCTGAGGACCCCTGGTATTGTCCAAATTGTAAAGAGCATCAGCAAGCCACAAAGAAATTAG ATCTGTGGTCCCTGCCTCCAGTTCTTGTGGTGCATCTCAAGAGATTCTCCTATAGTCGCTACATGAGAGA CAAGCTAGATACATTAGTCGATTTTCCCATCAGTGACTTGGATATGTCAGAATTCCTAATTAACCCAAAT GCAGGTCCTTGCCGCTATAATTTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACATTATA CTGCTTTTGCAAAAAACAAAGATGATGGGAAGTGGTACTATTTCGATGACAGTAGCGTCTCCACTGCATC TGAAGACCAAATCGTGTCCAAGGCAGCGTACGTGCTCTTCTACCAGAGACAGGACACTTTCAGTGGGACT GGCTTCTTCCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACAGGTATCCCCCTGGAAAGTGACG AAGACAGCAATGATAATGACAATGACCTAGAAAATGAAAACTGTATGCACACTAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301628
Insert Size	2859 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301628.1, NP_001288557.1
RefSeq Size	11477 bp
RefSeq ORF	2859 bp
Locus ID	14479
UniProt ID	Q8R5H1
Gene Summary	The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms.[provided by RefSeq, Aug 2014] Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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