Pkn1 (NM_001199593) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	DBK; F730027O18Rik; PAK1; Pkn; PRK1; Prkcl1; Stk3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229206 representing NM_001199593 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTGATCCCCCTTTGGATTCGGAGCTGGAGAGTGAGCCTCGAAGCTGGTCGCTGCTGGAACAGC TGGGTCTGGCTGGGGCTGACCTGGCAGCCCCCGGGGTGCAGCAACAGCTGGAGTTAGAGCGAGAGCGACT GAAGCGGGAGATACGGAAGGAGCTGAAGCTGAAGGAGGGCGCTGAGAACCTGAGGCGAGCCACCACTGAC CTGGGCCGTAGCCTGGCCCCTGTGGAGCTGCTGCTGCGGGGCTCCGCTCGACGCCTAGACTTACTGCACC AGCAGCTGCAGGAGCTGCATGCACATGTGGTGCTGCCTGACCCTGCAGCTGGAAGCGATGCTACCCAATC CCTTGCAGAGGGCAGCCCTATCTGCTCATCCACCAACCTGAGCAGAGTGGCTGGGCTGGAGAAGCAGCTG GCCATTGAGCTCAAGGTCAAACAGGGGGCAGAGAACATGATCCAGACCTATAGCAATGGCAGCAGCAAGG ACCGGAAGCTGTTGTTGACAGCCCAGCAGATGCTGCAGGACAGTAAGACCAAGATTGACATCATCCGCAT GCAGCTTCGAAGAGCGCTCCAGGCACTACAGGCTGGAGAGCTGGAGAGTCAGGCAGCTCCTGATGAAGCC CAAGGAGATCCAGAACTGGGAGCCGTAGAGCTACGCATTGAGGAGCTACGACACCATTTTCGAGTGGAGC ATGCAGTGGCAGAAGGTGCCAAGAATGTCCTGCGCCTGCTCAGTGGGGCAAAGGCCCCAGACCGCAAGGC AGTCAGCGAGGCTCAGGAGAAATTGACTGAGTCCAACCAGAAGCTGGGCTTGCTGCGGGAATCACTGGAG AGGCGCCTTGGGGAGTTGCCTGCCGATCACCCCAAGGGGCGGCTGCTTCGGGAGGAGCTCACTGCAGCCT CGTCCTCAGCCTTCAGCGCCATACTGCCTGGGCCCTTCCCTGCCACTCACTACAGCACCTTGAGCAAGCC TGCACCGCTCACAGGGACCCTGGAAGTACGAGTGGTGGGCTGCAAAAACCTTCCCGAGACCATCCCCTGG AGCCCTCCCCCCTCAGTGGGGGCATCTGGGACCCCCGAAAGCCGCACTCCGTTCCTGAGTCGCCCCGCTC GGGGCCTTTACAGCCGGAGTGGAAGCCTTAGTGGACGGAGCAGCCTCAGAGGGGAGGCAGAGAATGCCAC TGAGGTCAGCACCGTGCTCAAGCTGGACAACACAGTGGTGGGGCAAACAGCCTGGAAGCCATGCGGCCCC AATGCCTGGGACCAGAGCTTTACCCTGGAGCTGGAGAGGGCTCGGGAGCTGGAGCTGGCTGTGTTCTGGC GAGACCAGAGGGGTCTGTGTGCTCTCAAATTTCTGAAGTTGGAGGACTTCTTGGACAACGAGAGGCATGA GGTGCAGCTGGACATGGAACCCCAGGGCTGCCTGGTGGCTGAGGTCACTTTCCGAAACCCCATCATCGAG CGGATCCCTAGGCTCCAAAGGCAGAAAAAAATCTTCTCCAAGCAGCAAGGGAAGGCATTTCAGCGAGCCA GACAGATGAACATAGATGTGGCGACTTGGGTGCGGCTGCTCCGTAGACTCATCCCTAGTGCTGTGGCCAC TGGCACCTTCAGTCCCAATGCATCTCCAGGTGCTGAGATCCGGCACACTGGAGACATATCCATGGAGAAA TTGAATCTCGGTGCTGACTCAGACAGCTCGTCCCAAAAGAGCCCACCAGGGCTGCCCTCCACCTCATGCA GCCTGAGTTCTCCAACCCATGAATCCACCACATCTCCAGAGCTGCCTTCAGAGACCCAGGAGACTCCAGG CCCTGGCCTGTGCAGCCCCTTGAGAAAGTCGCCCCTGACACTTGAGGACTTCAAGTTCCTGGCCGTGCTT GGCCGGGGTCACTTTGGAAAGGTGCTGCTGTCTGAATTCCGCTCCAGTGGGGAGCTCTTTGCCATCAAAG CCTTGAAGAAAGGTGACATTGTAGCCCGAGATGAGGTTGAGAGCCTGATGTGTGAGAAGCGGATTTTGGC GGCCGTGACCAGGGCAGGACATCCCTTCCTGGTGAACCTTTTCGGCTGTTTCCAGACCCCAGAGCACGTG TGCTTTGTGATGGAGTACTCGGCGGGTGGAGACCTGATGCTGCACATTCATAGCGACGTGTTCTCAGAGC CTCGGGCTGTCTTCTATTCGGCCTGTGTGGTGCTGGGACTGCAGTTCCTCCATGAACACAAGATTGTCTA CAGGGACCTGAAGTTGGACAATTTGCTCCTGGATACTGAGGGCTACGTCAAGATCGCAGACTTTGGCCTC TGCAAGGAGGGGATGGGCTATGGGGACCGGACCAGCACGTTCTGCGGAACTCCGGAGTTCCTGGCGCCGG AAGTGCTCACAGACACATCCTACACGCGAGCAGTGGACTGGTGGGGACTGGGCGTGCTGCTCTATGAGAT GTTGGTTGGAGAGTCTCCGTTCCCTGGGGATGATGAGGAGGAGGTATTTGACAGCATTGTCAACGACGAA GTTCGCTATCCCCGCTTCCTGTCTGCAGAGGCCATCGGCATCATGAGAAGGCTACTGCGGAGGAACCCGG AGCGGAGGCTGGGGTCCACTGAGCGCGATGCAGAAGATGTGAAAAAACAGCCTTTCTTCCGGTCTCTGGG CTGGGATGTCCTGCTGGCCCGCCGCTTGCCTCCACCCTTCGTGCCTACACTTTCAGGGCGCACAGATGTC AGCAACTTCGATGAGGAGTTCACTGGGGAGGCCCCCACACTGAGTCCTCCCCGGGATGCACGGCCCCTCA CAGCTGCGGAGCAGGCAGCCTTCCGGGATTTCGACTTTGTGGCCGGAGGCTACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199593
Insert Size	2856 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199593.1, NP_001186522.1
RefSeq Size	3325 bp
RefSeq ORF	2856 bp
Locus ID	320795
UniProt ID	P70268
Gene Summary	PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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