Sytl2 (NM_001289584) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI266830; mKIAA1597; Slp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229180 representing NM_001289584 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGACTTAAGTTTCCTGACAGAGGAGGAGCAAGACGCCATCTTAAAGGTTCTGCAGAGGGATGCTG CCCTGAAGAGGGCTGAAGAGGAGAGAGTCAGACATCTGCCTGAAAAAATTAAGGATGACCAACAACTAAA GAATATGAGTGGCCAATGGTTTTATGAAGCTAAGGCAAAAAGACACAGGGACAAAATCCACGGTGCTGAC ATCATCAGAGCATCCATGAGAAGGAAGAAGCTCCCAGCGGCAGAGATAGCTGTTGTGGAAGAGCCAGAAG ATGACACAGATCCCGCAGGCCCAAGTTCCAGTTTGGTGGATCCAGCTTCCAGTGTGATTGATATGTCCCA GGAAAGTACGAGGACTCCAGCTGTGTCTCTGCCCAAGCAAAGGAAGAATCCATTCAACAGCCCCAAGCTG CCGGAAGATCACTCCTTGCAACAAACCAAACCCGAGCAGTCAAAAACTGGAAAAGCTGGTTTATTTCAGA TTTCAAAAGAGGGTGAGTTGTCAGAATCAAAAGAAAAGTCATCTATCCCAGATATGCCACGTCAGCAGTT AGAGAAACCAAAGCAAACTGTATCCACAGAGCCTGAAAATGCGTCTCATACCAAAGCTCCAATCCCCAAA GCCAGAAAACTGATCTACAAATCAAATGACTTAGAGAAAGATGATAACCAGTCTTTTCCCAGACAACGGA GGGACTCCCTGAATGCGAGAGGGGCTCCAAGAGGGATCCTAAAGCGCAACTCCAGTTCCAGCAGCACGGA CTCAGAGACCCTCCGTTTAAATTACAACCTTGATCCGAAAAGCAAAATCCTATCACCTGGCCTAACCATC CACGAGAGAATCTCTGAGAAGGAATTTTCTTTAGAAGACGACTCTTCCACAAGCTCACTAGAGCCGTTAA AGCACGTGAGATTCTCTGCCGTGAAGAATGAGCTTCCACAAAGTCCTAGACCCGTCCTTGGCCAGGAAGT GGGAGAATTTACTGTTTTAGAATCTGACCAGTTGCAAAATGGAACTGAAGATGCAGGGGACATAGAGGAG TTTCAGAATCACCCAGAGCTCTCCCACAAAACACCTTTGTCTCATTATCAGTTAGTGTCAAGCCCAAGCG ATTCAGGAAGGGAAAGAGAACAGCTGATGTCTTCTGGGTCTGCTCCAAGAGATGAGATCCCTTGTCATTC AGACATTCTACCCACAGGACCTCAGTGTGTTGAGAGTTCATCCGTCATCAATGGGCAACAAGAGAAATCA TCACATTTTACAAAGCTTCCATCAGAATTGTCCAAAAGCCCTTCCGATGAACTGACTCAGTGTGGTGAGC CTGAGCCATCACAGACAGCAGACCACAGTTTTAGAGACCATCGGCAAGGTTCAGAAGAAGAGCACAGCCC TGTTTTGAAAACTCTGGAAAGAAGAGCTGCTAGGAAATTGCCTTCCAAAAGTCTAGAAGACATTCCATCA GATTCATCAAATCAAGCAAAAGTAGATAATCTGCCTGAAGAATTAGTGCGTAGTGCTGAAGATGATCAAA AAGCCGACCAAGAACCAGATACAAATGAATGTATACCAGGAATTTCCACAGTGCCTTCACTGCCTGATAA TCAGTTTTCCCACCCTGACAAACTCAAAAGGATGAGCAAGTCCGTGCCAGCATTTCTTCAAGATGAGAGC GATGACAGAGAAACAGACACAGCATCAGAGAGCAGCTACCAGCTCAGGAGATACAAGAAGAGCCCCAGCT CATTAACCAATCTTAGCAGCTCCTCTGGCATGACGTCCTTGTCCTCTGCGAGTGGCAGTGTGATGAGCGT TTACAGTGGAGACTTTGGCAACCTAGAAGTGAAAGGAAGCGTGCAGTTTGCACTCGACTACGTGGAGTCC CTGAAAGAGCTGCATGTGTTTGTGGCCCAGTGTAAGGATTTAGCAGCAGCAGATGTTAAGAAACAGCGCT CAGATCCGTATGTAAAGACCTATCTGCTACCAGACAAAGGCAAAATGGGCAAGAAGAAGACACTCGTAGT GAAGAAGACCTTGAATCCTGTATACAACGAGATATTGCGGTATAAAATTGAAAGGCAATTCTTAAAGACG CAGAAGTTGAACCTGTCCGTTTGGCATCGGGATACATTTAAGCGCAACAGCTTTCTGGGGGAGGTGGAGC TCGACCTGGAAACGTGGGATTGGGACAGCAAACAGAACAAACAGCTGAAGTGGTACCCACTGAAGAGGAA GACAGCACCAGTTGCCCTCGAGACAGAAAACAGAGGTGAAATGAAACTAGCTCTCCAGTATGTTCCGGAA CCAAGCCCTGGCAAAAAGCTTCCTACAACTGGAGAAGTCCACATCTGGGTGAAGGAATGCCTTGACCTCC CACTGTTGAGGGGCAGCCACCTAAATTCTTTTGTTAAATGTACCATCCTTCCAGATACCAGTAGAAAAAG TCGCCAGAAGACAAGAGCTGTAGGGAAAACCACCAACCCCGTCTTCAACCATACCATGGTGTATGATGGG TTCAGGCCTGAAGATCTGATGGAAGCCTGTGTAGAACTCACAGTCTGGGACCATTATAAACTAACCAACC AGTTTCTGGGAGGTCTCCGGATCGGCTTTGGAACAGGAAAAAGCTACGGGACTGAAGTGGATTGGATGGA TTCTACTTCTGAGGAAGTTGCTCTCTGGGAGAAGATGGTAAACTCTCCCAACACTTGGGTTGAAGCGACG CTGCCCCTCCGGATGCTTCTGATTGCCAAGCTTTCCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289584
Insert Size	2772 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289584.1, NP_001276513.1
RefSeq Size	4698 bp
RefSeq ORF	2772 bp
Locus ID	83671
UniProt ID	Q99N50
Gene Summary	Isoform 11 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. Required for cytotoxic granule docking at the immunologic synapse. Isoform 1 may play a role in melanosome transport and vesicle trafficking. It controls melanosome distribution in the cell periphery and regulates melanocyte morphology. Isoform 1 acts as a positive mediator of mucus secretion by the surface mucus cells of the stomach. Mediates basal mucus secretion by gastric surface cells by promoting the proper granule biognesis and docking of mucus granules with the apical plasma membrane.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (7) uses an alternate in-frame splice site in the 5' coding region, compared to variant 6, resulting in an isoform (7, also known as Slp2-a delta 2S-I) that is shorter than isoform 6.

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