Cask (NM_001284505) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	DXPri1; DXRib1; LIN-2; mLin-2; Pals3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229175 representing NM_001284505 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACGACGACGTGCTGTTCGAGGATGTGTACGAGCTATGCGAGGTGATCGGCAAGGGTCCCTTCA GTGTTGTACGGCGATGTATCAACAGAGAAACTGGGCAACAATTTGCTGTAAAAATTGTTGATGTAGCCAA GTTCACATCAAGTCCAGGGTTAAGTACAGAAGATCTAAAGCGGGAAGCCAGTATCTGTCATATGCTGAAG CATCCACACATTGTAGAGCTGTTGGAGACATATAGCTCAGATGGGATGCTTTACATGGTTTTTGAATTTA TGGATGGAGCAGATCTGTGTTTTGAAATCGTAAAGCGAGCTGATGCTGGTTTTGTATACAGTGAAGCTGT AGCCAGCCACTACATGAGACAGATACTGGAAGCTCTGCGCTACTGTCATGATAATAACATAATTCACAGG GACGTGAAGCCCCACTGTGTTCTCCTTGCCTCAAAAGAAAACTCGGCACCTGTTAAACTTGGGGGCTTTG GGGTGGCCATTCAGTTAGGAGAATCTGGACTTGTTGCTGGAGGCCGCGTTGGAACACCTCACTTTATGGC CCCAGAAGTGGTCAAGAGAGAGCCTTACGGAAAGCCTGTGGATGTCTGGGGCTGTGGTGTGATCCTTTTC ATCCTGCTCAGTGGCTGTTTGCCTTTCTACGGAACCAAGGAAAGATTGTTTGAAGGCATTATTAAAGGAA AATATAAGATGAATCCAAGGCAGTGGAGCCATATCTCTGAAAGTGCCAAAGACCTAGTACGCCGCATGCT GATGCTGGATCCTGCTGAAAGGATCACTGTTTATGAAGCACTGAATCACCCATGGCTTAAGGAGCGGGAT CGTTATGCCTACAAAATCCATCTTCCAGAAACAGTAGAACAACTGAGGAAATTCAATGCAAGGAGAAAAC TAAAGGGTGCAGTACTAGCTGCTGTGTCAAGTCACAAATTCAATTCCTTCTATGGGGACCCTCCTGAAGA GTTGCCAGATTTCTCCGAAGACCCTACCTCCTCAGGAGCCGTCTCTCAGGTGCTGGACAGCCTGGAAGAG ATTCACGCACTTACAGACTGCAGTGAAAAGGACCTAGATTTTCTACACAGTGTTTTCCAGGATCAACATC TTCACACACTGCTGGATCTGTATGACAAAATTAACACAAAGTCTTCGCCACAAATCAGGAATCCTCCAAG TGATGCAGTACAGAGAGCCAAAGAGGTATTGGAAGAAATTTCATGTTACCCTGAGAATAATGATGCGAAG GAACTAAAGCGTATTTTAACACAACCTCATTTCATGGCCTTACTTCAGACTCATGATGTAGTGGCACATG AAGTTTACAGTGATGAAGCATTAAGGGTCACACCTCCCCCGACTTCCCCCTATTTAAACGGTGATTCTCC AGAAAGTGCAAACGGAGACATGGACATGGAGAATGTGACCAGAGTTCGGCTGGTACAGTTCCAAAAGAAC ACGGATGAGCCAATGGGAATCACTTTGAAAATGAATGAGCTAAATCATTGTATTGTCGCAAGAATCATGC ATGGGGGTATGATTCACAGGCAAGGTACACTTCATGTTGGTGATGAAATCCGAGAAATCAATGGCATCAG TGTCGCTAACCAAACAGTGGAACAGCTACAGAAAATGCTTAGGGAAATGCGAGGGAGTATTACCTTCAAG ATTGTGCCAAGCTACCGCACTCAGTCTTCGTCCTGTGAGAGAGATTCCCCCTCCACTTCCAGACAGTCCC CTGCTAATGGTCATAGCAGCACTAACAATTCTGTTTCGGACTTGCCATCAACCACCCAACCAAAAGGACG ACAGATCTATGTAAGAGCACAATTTGAATATGATCCAGCCAAGGATGACCTCATCCCCTGCAAAGAAGCT GGCATCCGGTTCCGAGTTGGTGACATCATCCAGATTATTAGTAAGGATGACCACAACTGGTGGCAGGGTA AACTGGAAAACTCCAAAAATGGAACTGCAGGTCTCATTCCTTCTCCTGAACTTCAGGAATGGCGAGTAGC TTGCATTGCCATGGAGAAGACCAAACAAGAGCAGCAGGCCAGCTGTACTTGGTTTGGCAAGAAAAAGAAG CAGTACAAAGATAAATATTTGGCAAAGCACAACGCAGTGTTTGATCAATTAGATCTTGTCACATATGAAG AAGTAGTCAAACTGCCAGCATTCAAAAGGAAAACATTAGTCTTATTAGGTGCACATGGTGTTGGAAGAAG ACACATAAAAAATACCCTCATCACAAAGCACCCGGACCGGTTTGCGTACCCTATTCCACATACAACCAGA CCTCCAAAGAAAGATGAAGAAAATGGCAAGAATTATTACTTTGTATCTCATGACCAAATGATGCAAGACA TCTCAAATAACGAATACTTGGAGTATGGCAGCCATGAGGATGCAATGTACGGGACAAAACTGGAGACCAT TCGGAAAATCCATGAGCAGGGGCTGATTGCGATTCTGGACGTGGAGCCTCAGGCACTGAAGGTCCTGAGG ACTGCAGAGTTTGCTCCTTTTGTTGTCTTCATTGCGGCGCCAACTATCACTCCAGGTTTAAATGAGGATG AATCTCTTCAGCGCCTGCAGAAGGAGTCCGATGTCTTGCAGAGAACATATGCACACTACTTCGATCTCAC AATTATCAACAACGAAATTGATGAGACAATCAGACATCTGGAAGAAGCTGTCGAGCTTGTGTGCACAGCC CCACAGTGGGTCCCAGTCTCCTGGGTCTATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284505
Insert Size	2763 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284505.1, NP_001271434.1
RefSeq Size	8358 bp
RefSeq ORF	2763 bp
Locus ID	12361
UniProt ID	O70589
Gene Summary	Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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