Tjp3 (NM_001282096) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229167 representing NM_001282096 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGAGATTCCAGGTGGCAGACATGGAAGAGCTGACCATCTGGGAACAACACACAGCCACACTGT ACAAGGACCCCCGCCGCGGCTTCGGCATAGCTGTCTCTGGAGGCCATGACCGAGCTAGTGGATCCGTGGT GGTGTCTGATGTGGTACCAGGAGGCCCCGCTGAGGGCCGGCTCCGGACCGGGGACCACATCGTCATGGTG AATGGTGTCTCTGTGGAGAACGTCACATCTGCCTTTGCCATCCAGATCCTTAAGACCTGCACCAAAACAG CCAACGTTACAGTGAAGCGTCCCCGCAGGGTCCAGCTGCCTGCCACCAAGGCTAGCCCAGCTTCTGGCCA CCAGCTCTCAGACCAGGAAGAAGCTGACCATGGCCGGGGCTATGAGGGGGACTCCTCCAGTGGCTCAGGT CGATCATGGGGTGAGCGTTCTCGCCGGTCCAGGGCAGGTCGCCGCGGCAGGGTAGGCAGCCATGGGCGCC GGAGTTCGGGTGGCGGATCTGAAGCCAACGGTCTGGACCTGGTGTCTGGCTATAAGCGCTTGCCGAAGCA GGATGTGCTAATGAGGCCACTAAAGTCCGTGCTGGTGAAACGAAGAAACAGCGAAGAGTTTGGGGTGAAG CTAGGCAGTCAGATCTTTATAAAACACATCACGGAATCTGGCCTTGCTGCTCGGAACCATGGGCTGCAGG AGGGAGACCTTATCTTACAGATCAACGGGGTGTCCAGTGCTAATCTGTCCCTGAGTGACACACGAAGGCT CATTGAGAAGTCGGAGGGGGAGCTGACGTTGCTGGTGCTAAGAGACAGTGGGCAGTTCCTGGTGAACATC CCTCCCGCAGTGAGCGACAGCGACAGCTCCCTAATGGAAGACATCTCAGACCTGACCTCAGAATTGTCCC AGGCGCCCCCTTCCCACGTTCCTCCGCCACCTCTGAAGGGTCAGAGGAGCCCTGAGGACAGCCAGACAGA CTCCCCAGTGGAGACCCCGCAGCCCCGGAGGCGGGAACGGTCAGTGAATTCAAGAGCCATCGCTGAACCA GAGTCTCCTGGAGAAAGCCGCTATGACATTTACAGGGTTCCTAGCCGGCAGAGCCTGGAGGACCGTGGCT ACAGCCCCGACACTCGTGTCGTGAGCTTCCCCAAGGGCGCGAGTATTGGCTTGCGGCTGGCCGGCGGCAA CGACGTGGGCATCTTCGTATCCGGGGTGCAGGCAGGCAGCCCGGCCGACGGGCAAGGCATCCAGGAAGGG GATGAGATCTTGCAGGTGAATGGTATGCCATTTCGGAACCTGACTCGGGAGGAGGCCGTGCAGTTTCTGT TGGGGCTGCCTCCAGGGGAAGACATGGAGCTGGTAACACAGAGCAAACAGGACATCTTTAAGAAGATGGT CCAGTCTCGCGTGGGTGACTCCTTCTACATCCGCACACACTTTGAGCTGGAGCCGAGCCCACCCTATGGC CTGGGCTTCACCCGCGGTGATGTCTTCCACGTAGTGGACACGTTGTACCCGGGCTCCGGGCCAGGCCATG GGCACAGCAGCCATGGGGGCCTCTGGCTGGCCGCCCGCATGGGCAGAGATCTGCGAGAGCAAGAACGCGG CGTCATCCCCAACCAGAGCAGGGCCGAGCAGCTGGCCAGTCTGGAGGCTGCGCAGCGGGCCGCGGGCGTG GGGCCAGGCGCCTCAGTGGGTTCCAACCCACGGGCGGAGTTTTGGCGACTCCGAGGTCTCCGCCGAGGGA CCAAGAAGGCGTCGACCCAGCGGAGCCGCGAGGACCTATCAGCGCTGACCAGGCAGGGACACTACCCGCC CTATGAGCGTGTGGTGCTCAGAGAAGCCAGTTTTAAGCGCCCCGTGGTGATCCTAGGACCAGTGGCAGAC ATTGCCATGAAGAAACTGACCACAGAGATGCCAGAAGAGTTTGAGATTGCAGAAAGCATGTCCCGGACTG ACAGCCCATCCAAGATCATCAAACTGGACACAGTGCGGGTGATCGCAGAGAGGGATAAGCACGCACTCCT GGATGTCACCCCCTCTGCCATCGAGCGCCTCAACTACGTGCAGTACTACCCCATCGTCATCTTTTGTGCC CCAGAGAGCCGGCCGGCCCTCAAGGCCCTCCGGGAGTGGCTGGCCCCGGCCTCTCGCCGCAGCAGCCGCC GCCTGTATGCGCAGGCCCAGAAACTGCAGAAACACAGTGGCCACCTCTTCACCGCCACCATCCCCCTGCA TGGCACTAGTGACTCCTGGTACCAGGAAGTCAAGGCTGTAATACAGCAACAGCAGGCGCGACCGATCTGG ACCGCGGAGGACCAGCTGAACAGCTCATCCGAGGACCTTGACCTGCCGGGCCATGGTCTGGCCGCCAGCT CTGGAGACCTCAGCTGTGACAGTCGGACCAACAGCGACTATGAGGACACCGACGGCGAGGGCGCCTATAC GGACAGAGAGGGCGGACCCCAGGATGTCGATGAGGAGGTCGCACCCACAGCCCTGGCCCGGTCCTCAGAG CCCGTATGGGTAGATGATCACCAGGGGCTGATGGGTCATGGGACAACTATCACAGACAAATGGGAGACAC AGGCTGACAGCCACTACACCCAGGACCAGCGGCGCCAGGACAGCATGCGGACCTACAAGCACGAGGCCCT GAGGAAGAAGTTCACAAGAGCCCGGGATGTGGAATCCTCTGATGACGAGGGCTATGACTGGGGCCCAGCC ACCGACCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282096
Insert Size	2742 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282096.1, NP_001269025.1
RefSeq Size	3004 bp
RefSeq ORF	2742 bp
Locus ID	27375

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