Grm4 (NM_001291045) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gprc1d; mGluR4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229165 representing NM_001291045 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGGAAGGGAGGCTGGGCCTGGTGGTGGGCCCGGCTGCCCCTCTGCCTACTCCTCAGCCTTTATG GCTCCTGGGTGCCTTCATCCCTAGGAAAGCCCAAGGGTCACCCCCACATGAACTCTATCCGTATCGATGG AGACATCACCCTGGGAGGCCTGTTTCCCGTCCACGGTCGCGGCTCCGAGGGCAAGGCCTGCGGCGAGTTG AAGAAGGAGAAAGGTATCCACCGGCTGGAGGCCATGCTCTTTGCCCTGGACCGCATCAACAACGACCCGG ACCTACTGCCCAACATCACGTTGGGCGCCCGCATTCTGGACACCTGCTCAAGGGATACCCACGCCCTGGA GCAGTCCCTGACCTTTGTGCAGGCGCTCATCGAGAAGGACGGCACGGAGGTCCGCTGCGGCAGCGGGGGC CCACCCATCATCACCAAGCCTGAACGAGTGGTGGGTGTCATCGGAGCTTCGGGGAGCTCCGTCTCGATCA TGGTGGCCAACATCCTCCGCCTCTTCAAGATCCCCCAGATCAGCTACGCCTCCACGGCTCCCGACTTGAG TGATAACAGCCGCTATGACTTCTTCTCCCGGGTCGTGCCCTCGGACACATACCAGGCCCAGGCCATGGTG GACATCGTCCGGGCCCTCAAGTGGAACTATGTGTCCACGCTGGCCTCAGAGGGTAGCTACGGCGAGAGCG GCGTGGAGGCCTTTATCCAGAAGTCCCGAGAGAACGGAGGCGTGTGCATTGCCCAGTCGGTGAAGATTCC ACGGGAACCCAAGACCGGGGAGTTTGACAAGATCATCAAACGCCTTCTGGAAACGTCCAATGCCAGAGCC ATCATCATCTTTGCCAACGAGGATGATATCAGGAGGGTGCTGGAGGCAGCGCGCAGGGCCAACCAGACCG GCCACTTCTTTTGGATGGGTTCTGATAGCTGGGGCTCCAAGAGCGCCCCCGTGCTGCGCCTTGAGGAAGT GGCTGAAGGTGCAGTCACCATTCTTCCCAAGAGGACGTCTGTGCGAGGGTTTGACCGATACTTCTCCAGC CGCACGCTTGACAACAACAGGCGCAACATCTGGTTTGCTGAGTTCTGGGAGGACAACTTCCATTGCAAGT TGAGCCGCCACGCGCTCAAGAAGGGAAGCCACATCAAGAAGTGCACCAACCGAGAGCGCATCGGGCAGGA CTCGGCCTACGAACAGGAGGGGAAGGTGCAGTTTGTGATCGACGCCGTGTACGCCATGGGCCATGCTCTG CACGCCATGCATCGTGACCTGTGTCCCGGCCGCGTAGGACTCTGCCCTCGAATGGACCCTGTGGATGGCA CCCAGCTGCTTAAGTACATCAGAAACGTCAACTTCTCAGGCATCGCCGGGAACCCGGTGACCTTCAACGA GAACGGAGACGCGCCAGGGCGTTATGACATCTACCAGTACCAACGTCGCAACGGCTCGGCTGAGTACAAG GTCATCGGCTCATGGACAGACCACTTGCACCTCAGAATAGAGCGGATGCAGTGGCCAGGGAGTGGCCAGC AGCTGCCACGCTCCATCTGCAGCCTGCCCTGCCAGCCAGGCGAGCGGAAGAAGACGGTGAAGGGCATGGC TTGCTGCTGGCACTGCGAGCCCTGCACGGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACCTGC CCCTATGACATGCGGCCCACGGAGAACCGCACGAGCTGCCAGCCCATACCCATTGTCAAGTTGGAGTGGG ACTCACCCTGGGCTGTGCTGCCCCTCTTCCTGGCTGTGGTGGGCATTGCTGCCACGCTGTTCGTGGTGGT CACTTTTGTGCGCTACAACGACACTCCGATCGTCAAGGCCTCGGGCCGGGAGCTGAGCTACGTCCTGCTG GCGGGCATCTTTCTCTGCTATGCCACCACCTTCCTCATGATCGCAGAGCCTGACCTGGGGACCTGTTCAC TCCGCCGCATCTTCCTGGGGCTTGGCATGAGCATCAGCTACGCGGCCCTGCTGACCAAGACCAACCGCAT CTACCGCATCTTTGAGCAGGGCAAGCGGTCGGTCAGCGCCCCACGGTTCATCAGCCCCGCCTCGCAGCTG GCCATCACCTTCGTCCTCATCTCGCTGCAGTTGCTTGGCATCTGCGTGTGGTTCGTGGTGGACCCCTCCC ACTCGGTGGTGGACTTCCAGGACCAGCGGACACTTGACCCCCGCTTCGCCCGGGGTGTGCTCAAGTGTGA CATCTCGGACCTGTCGCTCATCTGCCTCCTGGGCTACAGCATGCTGCTGATGGTCACGTGTACTGTGTAT GCCATCAAGACTCGAGGCGTGCCTGAGACCTTCAATGAGGCCAAGCCCATCGGCTTCACCATGTACACCA CCTGCATCGTCTGGCTGGCCTTCATCCCCATCTTTTTTGGCACCTCGCAGTCGGCTGACAAGCTGTACAT CCAGACAACCACACTGACGGTCTCTGTGAGTCTGAGCGCTTCAGTGTCCCTGGGGATGCTCTACATGCCC AAAGTCTACATCATCCTCTTCCACCCGGAGCAGAACGTGCCCAAGCGCAAGCGCAGCCTCAAAGCCGTTG TCACCGCCGCCACCATGTCCAACAAGTTCACACAGAAGGGCAACTTCAGGCCTAATGGGGAAGCCAAGTC AGAGCTGTGTGAGAACCTGGAGGCCCCAGCACTGGCCACCAAACAGACCTACGTCACTTACACCAACCAT GCAATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001291045
Insert Size	2739 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291045.1, NP_001277974.1
RefSeq Size	3987 bp
RefSeq ORF	2739 bp
Locus ID	268934
Gene Summary	G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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