Grm4 (NM_001291045) Mouse Untagged Clone

CAT#: MC229165

Grm4 (untagged) - Mouse glutamate receptor, metabotropic 4 (Grm4), transcript variant 1



  "NM_001291045" in other vectors (1)

CNY 6,688.00

CNY 8,740.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gprc1d; mGluR4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229165 representing NM_001291045
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGGAAGGGAGGCTGGGCCTGGTGGTGGGCCCGGCTGCCCCTCTGCCTACTCCTCAGCCTTTATG
GCTCCTGGGTGCCTTCATCCCTAGGAAAGCCCAAGGGTCACCCCCACATGAACTCTATCCGTATCGATGG
AGACATCACCCTGGGAGGCCTGTTTCCCGTCCACGGTCGCGGCTCCGAGGGCAAGGCCTGCGGCGAGTTG
AAGAAGGAGAAAGGTATCCACCGGCTGGAGGCCATGCTCTTTGCCCTGGACCGCATCAACAACGACCCGG
ACCTACTGCCCAACATCACGTTGGGCGCCCGCATTCTGGACACCTGCTCAAGGGATACCCACGCCCTGGA
GCAGTCCCTGACCTTTGTGCAGGCGCTCATCGAGAAGGACGGCACGGAGGTCCGCTGCGGCAGCGGGGGC
CCACCCATCATCACCAAGCCTGAACGAGTGGTGGGTGTCATCGGAGCTTCGGGGAGCTCCGTCTCGATCA
TGGTGGCCAACATCCTCCGCCTCTTCAAGATCCCCCAGATCAGCTACGCCTCCACGGCTCCCGACTTGAG
TGATAACAGCCGCTATGACTTCTTCTCCCGGGTCGTGCCCTCGGACACATACCAGGCCCAGGCCATGGTG
GACATCGTCCGGGCCCTCAAGTGGAACTATGTGTCCACGCTGGCCTCAGAGGGTAGCTACGGCGAGAGCG
GCGTGGAGGCCTTTATCCAGAAGTCCCGAGAGAACGGAGGCGTGTGCATTGCCCAGTCGGTGAAGATTCC
ACGGGAACCCAAGACCGGGGAGTTTGACAAGATCATCAAACGCCTTCTGGAAACGTCCAATGCCAGAGCC
ATCATCATCTTTGCCAACGAGGATGATATCAGGAGGGTGCTGGAGGCAGCGCGCAGGGCCAACCAGACCG
GCCACTTCTTTTGGATGGGTTCTGATAGCTGGGGCTCCAAGAGCGCCCCCGTGCTGCGCCTTGAGGAAGT
GGCTGAAGGTGCAGTCACCATTCTTCCCAAGAGGACGTCTGTGCGAGGGTTTGACCGATACTTCTCCAGC
CGCACGCTTGACAACAACAGGCGCAACATCTGGTTTGCTGAGTTCTGGGAGGACAACTTCCATTGCAAGT
TGAGCCGCCACGCGCTCAAGAAGGGAAGCCACATCAAGAAGTGCACCAACCGAGAGCGCATCGGGCAGGA
CTCGGCCTACGAACAGGAGGGGAAGGTGCAGTTTGTGATCGACGCCGTGTACGCCATGGGCCATGCTCTG
CACGCCATGCATCGTGACCTGTGTCCCGGCCGCGTAGGACTCTGCCCTCGAATGGACCCTGTGGATGGCA
CCCAGCTGCTTAAGTACATCAGAAACGTCAACTTCTCAGGCATCGCCGGGAACCCGGTGACCTTCAACGA
GAACGGAGACGCGCCAGGGCGTTATGACATCTACCAGTACCAACGTCGCAACGGCTCGGCTGAGTACAAG
GTCATCGGCTCATGGACAGACCACTTGCACCTCAGAATAGAGCGGATGCAGTGGCCAGGGAGTGGCCAGC
AGCTGCCACGCTCCATCTGCAGCCTGCCCTGCCAGCCAGGCGAGCGGAAGAAGACGGTGAAGGGCATGGC
TTGCTGCTGGCACTGCGAGCCCTGCACGGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACCTGC
CCCTATGACATGCGGCCCACGGAGAACCGCACGAGCTGCCAGCCCATACCCATTGTCAAGTTGGAGTGGG
ACTCACCCTGGGCTGTGCTGCCCCTCTTCCTGGCTGTGGTGGGCATTGCTGCCACGCTGTTCGTGGTGGT
CACTTTTGTGCGCTACAACGACACTCCGATCGTCAAGGCCTCGGGCCGGGAGCTGAGCTACGTCCTGCTG
GCGGGCATCTTTCTCTGCTATGCCACCACCTTCCTCATGATCGCAGAGCCTGACCTGGGGACCTGTTCAC
TCCGCCGCATCTTCCTGGGGCTTGGCATGAGCATCAGCTACGCGGCCCTGCTGACCAAGACCAACCGCAT
CTACCGCATCTTTGAGCAGGGCAAGCGGTCGGTCAGCGCCCCACGGTTCATCAGCCCCGCCTCGCAGCTG
GCCATCACCTTCGTCCTCATCTCGCTGCAGTTGCTTGGCATCTGCGTGTGGTTCGTGGTGGACCCCTCCC
ACTCGGTGGTGGACTTCCAGGACCAGCGGACACTTGACCCCCGCTTCGCCCGGGGTGTGCTCAAGTGTGA
CATCTCGGACCTGTCGCTCATCTGCCTCCTGGGCTACAGCATGCTGCTGATGGTCACGTGTACTGTGTAT
GCCATCAAGACTCGAGGCGTGCCTGAGACCTTCAATGAGGCCAAGCCCATCGGCTTCACCATGTACACCA
CCTGCATCGTCTGGCTGGCCTTCATCCCCATCTTTTTTGGCACCTCGCAGTCGGCTGACAAGCTGTACAT
CCAGACAACCACACTGACGGTCTCTGTGAGTCTGAGCGCTTCAGTGTCCCTGGGGATGCTCTACATGCCC
AAAGTCTACATCATCCTCTTCCACCCGGAGCAGAACGTGCCCAAGCGCAAGCGCAGCCTCAAAGCCGTTG
TCACCGCCGCCACCATGTCCAACAAGTTCACACAGAAGGGCAACTTCAGGCCTAATGGGGAAGCCAAGTC
AGAGCTGTGTGAGAACCTGGAGGCCCCAGCACTGGCCACCAAACAGACCTACGTCACTTACACCAACCAT
GCAATCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001291045
Insert Size 2739 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001291045.1, NP_001277974.1
RefSeq Size 3987 bp
RefSeq ORF 2739 bp
Locus ID 268934
Gene Summary G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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