Ilf3 (NM_001277322) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MBII-26; MPHOSPH4; NF9; NF90; NFAR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229162 representing NM_001277322 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTGTATCATCATCACTTCATCACAAGAAGAAGAAGGCGTCCCATGAGAATTTTTGTGAATGATG ATCGCCACGTGATGGCAAAGCATTCTTCAGTGTACCCAACACAAGAGGAGCTGGAGGCTGTACAGAACAT GGTGTCCCATACTGAGCGGGCCCTGAAGGCTGTCTCTGACTGGATTGATGAGCAGGAGAAAGGCAACAGC GAGCTCTCTGAGGCAGAAAATATGGACACACCCCCAGACGATGAGAGCAAAGAAGGGGCTGGGGAACAGA AGGCGGAACACATGACTAGGACCCTGAGGGGCGTGATGCGGGTCGGCCTGGTAGCCAAGGGTCTTCTGCT CAAGGGGGACTTGGATCTGGAGCTGGTTCTGCTCTGTAAGGAGAAGCCCACAACCGCCCTTCTGGACAAG GTGGCTGACAACCTGGCCATCCAGCTCACTACTGTAACAGAAGACAAGTATGAAATACTCCAGTCTGTGG ATGATGCTGCGATTGTGATAAAAAACACAAAAGAGCCCCCCTTGTCCTTGACCATCCATCTGACCTCCCC TGTTGTCAGAGAAGAAATGGAGAAAGTATTAGCTGGAGAAACGCTATCAGTCAACGATCCCCCGGACGTT CTGGACAGGCAGAAATGCCTTGCTGCCTTGGCGTCCCTCCGACACGCCAAGTGGTTCCAGGCCAGAGCCA ATGGACTGAAGTCATGTGTCATTGTCATCCGTGTCCTAAGGGACTTGTGTACCCGAGTGCCCACCTGGGG TCCCCTCAGAGGATGGCCTCTGGAGCTGCTGTGTGAGAAGTCCATCGGCACTGCCAATAGGCCAATGGGT GCTGGTGAAGCCCTGCGGAGAGTGCTGGAGTGCCTGGCATCCGGCATCGTAATGCCAGATGGTTCTGGCA TTTATGACCCTTGTGAAAAAGAAGCCACTGATGCTATTGGGCATCTAGACAGACAGCAACGGGAAGATAT CACACAGAGTGCGCAGCATGCTCTGCGGCTTGCTGCCTTTGGTCAACTCCATAAAGTACTGGGAATGGAC CCCCTGCCTTCCAAAATGCCCAAGAAACCAAAGAACGAGAACCCGGTGGACTACACTGTTCAAATTCCTC CCAGCACCACCTATGCTATCACACCCATGAAACGCCCTATGGAAGAGGATGGGGAGGAGAAGTCTCCCAG CAAGAAGAAAAAGAAGATCCAGAAGAAAGAGGAGAAGGCTGATCCTCCTCAAGCTATGAATGCCCTGATG AGGTTAAATCAGCTGAAGCCAGGGCTGCAGTACAAGCTGATCTCCCAGACAGGCCCTGTTCATGCTCCCA TCTTCACCATGTCTGTGGAGGTAGACGGCAGTAACTTCGAGGCCTCGGGGCCATCTAAAAAGACTGCCAA GCTTCATGTAGCTGTGAAGGTGTTACAGGACATGGGCTTGCCAACAGGCGCTGAAGGCAGAGACTCCAGC AAGGGGGAAGACTCCGCTGAGGAGTCAGATGGGAAGCCAGCAATAGTGGCCCCACCCCCTGTGGTGGAAG CTGTCTCCAACCCCAGTTCTGTCTTCCCTTCAGATGCCACTACTGAGCAGGGACCGATTTTGACTAAGCA TGGCAAGAACCCTGTTATGGAGCTTAATGAGAAGAGACGTGGCCTCAAATATGAGCTCATTTCTGAGACG GGGGGCAGCCACGACAAAAGGTTTGTTATGGAGGTTGAGGTGGACGGACAGAAGTTTCAAGGTGCTGGTT CAAACAAAAAGGTGGCAAAGGCTTATGCTGCACTTGCCGCATTAGAAAAACTTTTCCCTGATACCCCTCT TGCTCTTGAAGCCAACAAAAAGAAAAGGACCCCAGTACCTGTCCGAGGTGGACCCAAATTTGCTGCCAAG CCACACAACCCTGGTTTTGGCATGGGAGGCCCCATGCATAATGAAGTGCCGCCACCTCCTAACATCCGAG GTCGGGGCCGAGGAGGTAACATCCGAGGGCGAGGACGGGGGCGAGGATTTGGTGGCGCCAACCATGGAGG AGGCTACATGAATGCTGGTGCTGGATATGGAAGCTATGGGTACAGCAGCAATTCGGCCACAGCAGGCTAC AGTCAGTTCTACAGCAATGGAGGGCATTCTGGGAATGCCGGTGGTGGAGGCAGCGGGGGAGGTGGTGGCT CATCCAGCTACAGCTCCTACTACCAAGGAGACAGCTACAACTCACCAGTACCCCCGAAGCATGCTGGGAA GAAGCCGCTGCATGGGGGCCAGCAGAAAGCCTCCTACAGCTCGGGCTACCAGTCCCACCAGGGCCAGCAG CAACCTTACAACCAGAGCCAGTACAGCAGCTACGGCACGCCACAGGGCAAGCAGAAAGGCTATGGCCATG GGCAGGGCAGCTACTCCTCCTACTCCAACTCTTACAACTCCCCAGGTGGTGGTGGGGGCTCTGACTACAG CTACGACAGCAAATTCAACTACAGTGGGAGTGGAGGCCGGAGTGGAGGCAACAGCTATGGCTCCAGCGGG TCATCGTCCTACAACACAGGCTCACATGGAGGCTATGGCACAGGCTCCGGAGGCAGCTCTTCATACCAAG GCAAACAAGGAGGCTACTCATCACAGTCAAACTACAGCTCACCTGGGTCCAGCCAGAGCTACAGTGGTCC TGCCAGCTCCTACCAGTCCTCACAGGGTGGCTACAGTCGGAACACAGAGCACAGCATGAACTACCAGTAC AGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277322
Insert Size	2736 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277322.1, NP_001264251.1
RefSeq Size	3531 bp
RefSeq ORF	2736 bp
Locus ID	16201
UniProt ID	Q9Z1X4
Gene Summary	The protein encoded by this gene contains two double-stranded RNA binding domains and functions in the post-transcriptional regulation of gene expression. It is a component of an RNA-protein complex that may be involved in mediating the export of messenger RNAs. Alternative splicing results in multiple transcript variants encoding distinct isoforms. These isoforms are grouped into two categories, NFAR-1 or NFAR-2, based on variation at the C-terminus. [provided by RefSeq, Mar 2013] Transcript Variant: This variant (6) differs in the 5' UTR, compared to variant 1. Both variants 1 and 6 encode the same isoform (1). This variant encodes an NFAR-2 isoform that is also known as Ilf3 L. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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