Dnmt3a (NM_001271753) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MmuIIIA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229155 representing NM_001271753 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCCTCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAG AGGAACAGGAGGAGAACCGTGGCAAGGAAGAGCGCCAGGAGCCCAGCGCCACGGCCCGGAAGGTGGGGAG GCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAGCAGTGACACCCCCAAGGACCCAGCAGTGACCACC AAGTCTCAGCCCATGGCCCAGGACTCTGGCCCCTCAGATCTGCTACCCAATGGAGACTTGGAGAAGCGGA GTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAGGGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGG AACTGAGACCCCACCAGAAGCCTCCAGAGCTGTGGAGAATGGCTGCTGTGTGACCAAGGAAGGCCGTGGA GCCTCTGCAGGAGAGGGCAAAGAACAGAAGCAGACCAACATCGAATCCATGAAAATGGAGGGCTCCCGGG GCCGACTGCGAGGTGGCTTGGGCTGGGAGTCCAGCCTCCGTCAGCGACCCATGCCAAGACTCACCTTCCA GGCAGGGGACCCCTACTACATCAGCAAACGGAAACGGGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCT GAGAAGAAAGCCAAGGTAATTGCAGTAATGAATGCTGTGGAAGAGAACCAGGCCTCTGGAGAGTCTCAGA AGGTGGAGGAGGCCAGCCCTCCTGCTGTGCAGCAGCCCACGGACCCTGCTTCTCCGACTGTGGCCACCAC CCCTGAGCCAGTAGGAGGGGATGCTGGGGACAAGAATGCTACCAAAGCAGCCGACGATGAGCCTGAGTAT GAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTGTGGGGGAAACTTCGGGGCTTCTCCTGGTGGCCAG GCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCCGAGCAGCTGAAGGCACTCGCTGGGTCATGTGGTT CGGAGATGGCAAGTTCTCAGTGGTGTGTGTGGAGAAGCTCATGCCGCTGAGCTCCTTCTGCAGTGCATTC CACCAGGCCACCTACAACAAGCAGCCCATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCA GCCGTGCCGGGAAGCTGTTTCCAGCTTGCCATGACAGTGATGAAAGTGACAGTGGCAAGGCTGTGGAAGT GCAGAACAAGCAGATGATTGAATGGGCCCTCGGTGGCTTCCAGCCCTCGGGTCCTAAGGGCCTGGAGCCA CCAGAAGAAGAGAAGAATCCTTACAAGGAAGTTTACACCGACATGTGGGTGGAGCCTGAAGCAGCTGCTT ACGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGAGCACAACAGAGAAACCTAAGGTCAAGGAGATCAT TGATGAGCGCACAAGGGAGCGGCTGGTGTATGAGGTGCGCCAGAAGTGCAGAAACATCGAGGACATTTGT ATCTCATGTGGGAGCCTCAATGTCACCCTGGAGCACCCACTCTTCATTGGAGGCATGTGCCAGAACTGTA AGAACTGCTTCTTGGAGTGTGCTTACCAGTATGACGACGATGGGTACCAGTCCTATTGCACCATCTGCTG TGGGGGGCGTGAAGTGCTCATGTGTGGGAACAACAACTGCTGCAGGTGCTTTTGTGTCGAGTGTGTGGAT CTCTTGGTGGGGCCAGGAGCTGCTCAGGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGCGGGC ATAAGGGCACCTATGGGCTGCTGCGAAGACGGGAAGACTGGCCTTCTCGACTCCAGATGTTCTTTGCCAA TAACCATGACCAGGAATTTGACCCCCCAAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATC CGCGTGCTGTCTCTCTTTGATGGGATTGCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGG ACCGCTACATTGCCTCCGAGGTGTGTGAGGACTCCATCACGGTGGGCATGGTGCGGCACCAGGGAAAGAT CATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGGAGTGGGGCCCATTCGACCTGGTG ATTGGAGGCAGTCCCTGCAATGACCTCTCCATTGTCAACCCTGCCCGCAAGGGACTTTATGAGGGTACTG GCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATGATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT CTTCTGGCTCTTTGAGAATGTGGTGGCCATGGGCGTTAGTGACAAGAGGGACATCTCGCGATTTCTTGAG TCTAACCCCGTGATGATTGACGCCAAAGAAGTGTCTGCTGCACACAGGGCCCGTTACTTCTGGGGTAACC TTCCTGGCATGAACAGGCCTTTGGCATCCACTGTGAATGATAAGCTGGAGCTGCAAGAGTGTCTGGAGCA CGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCATTACCACCAGGTCAAACTCTATAAAGCAGGGCAAA GACCAGCATTTCCCCGTCTTCATGAACGAGAAGGAGGACATCCTGTGGTGCACTGAAATGGAAAGGGTGT TTGGCTTCCCCGTCCACTACACAGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCG ATCGTGGAGCGTGCCGGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271753
Insert Size	2727 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271753.1, NP_001258682.1
RefSeq Size	9689 bp
RefSeq ORF	2727 bp
Locus ID	13435
UniProt ID	O88508
Gene Summary	This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1 and 3 encode the same isoform (1, also known as Dnmt3a1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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