Dlg1 (NM_001252435) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B130052P05Rik; Dlgh1; E-dlg/SAP97; mKIAA4187; SAP-97; SAP97
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229150 representing NM_001252435 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCATCTGTTGGAAGAATATCGATCGAAACTAAGCC AAACTGAAGACAGACAACTCAGAAGTTCCATAGAGCGGGTTATTAACATATTTCAGAGCAACCTCTTTCA GGCTTTAATAGATATTCAAGAATTTTATGAAGTGACCTTACTTGATAATCCAAAATGTGTGGATCATTCA AAGCAGTGTGAACCAGTTCAGCCTGTGACTACTTGGGAGATTGCCAGCCTTCCAAGCACTGCCGTGACGT CAGAAACCCTGCCCGGCAGCCTTAGCCCTCCAGTAGAGAAATACCGGTATCAGGATGAAGAGGTACTTCC TCCTGAGCATATTTCTCCACAAGTCACAAATGAGGTGCTAGGTCCAGAACTGGTCCATGTCTCAGAGAAG AACCTGTCAGAGATTGAGAATGTCCATGGATTTGTTTCTCATTCTCATATCTCACCAATAAAGCCCACAG AAGCTGTTCCTCCCTCCTCTCCCATTGTCCCTGTGACCCCTGCCCTGCCAGTCCCTGCTGAGAGTACTGT CGTCCTGCCCTCCGCACCACAGGCAAATCCTCCTCCAGTGCTGGTCAACACAGACAGCTTAGAGACACCA ACTTATGTTAATGGCACTGATGCAGATTATGAATATGAGGAAATCACACTTGAAAGGGGAAATTCGGGTC TTGGTTTCAGCATTGCAGGAGGTACAGACAACCCACACATTGGAGATGACTCAAGTATTTTCATCACCAA AATTATCACAGGCGGAGCGGCTGCCCAGGATGGAAGATTGCGGGTAAATGACTGTATACTGAGAGTAAAT GAAGCAGACGTTCGTGATGTAACCCACAGCAAAGCAGTGGAGGCATTAAAAGAAGCTGGATCTATTGTGC GATTGTATGTGAAAAGGCGGAAGCCAGCATCAGAAAAAATCATGGAAATAAAGCTCATTAAAGGTCCTAA AGGTCTTGGGTTCAGCATTGCTGGAGGTGTTGGAAATCAGCACATTCCTGGTGATAACAGCATCTATGTA ACCAAAATAATTGAAGGAGGTGCAGCACACAAGGATGGCAAGCTTCAGATTGGAGATAAGCTTCTAGCAG TGAACAGTGTGTGTTTAGAAGAAGTTACTCATGAAGAAGCAGTGACTGCCTTAAAGAATACATCTGATTT TGTTTATTTGAAAGTGGCAAAACCAACAAGTATGTATATAAATGATGGCTATGCACCACCTGACATCACT AATTCTTCTTCTCAATCTGTTGACAACCATGTCAGCCCGTCCTCCTGCTTGGGCCAGACGCCAACGTCAC CAGCCAGGTACTCACCCATTTCTAAAGCTGTGCTCGGAGATGACGAGATCACTAGGGAACCTAGAAAAGT TGTTCTTCATCGTGGCTCAACAGGACTTGGTTTTAACATTGTGGGAGGTGAAGATGGAGAAGGGATTTTT ATCTCCTTCATCCTTGCTGGCGGACCTGCTGATCTAAGTGGAGAGCTCAGAAAAGGAGATCGCATCATAT CGGTGAACAGTGTTGACCTCAGAGCTGCAAGTCACGAACAAGCAGCAGCTGCACTAAAGAACGCAGGCCA AGCCGTCACCATCGTTGCGCAATATCGACCCGAAGAGTACAGTCGTTTTGAAGCTAAAATCCATGACTTA CGGGAGCAGATGATGAATAGCAGTGTCAGTTCAGGGTCAGGGTCTCTTCGAACCAGCCAGAAGCGCTCCC TCTATGTCAGAGCCCTCTTTGATTATGACAAGACTAAGGACAGCGGGCTTCCCAGTCAAGGACTGAACTT CCGCTTTGGAGACATCCTCCATGTCATCAATGCTTCTGACGACGAGTGGTGGCAAGCCAGGCAGGTCACC CCAGACGGGGAGAGTGACGAAGTCGGAGTGATTCCTAGTAAACGAAGAGTTGAGAAGAAGGAACGAGCCC GATTAAAAACGGTCAAATTCAATTCTAAAACAAGAGGAGATAAAGGGGAGATCCCTGACGACATGGGATC AAAAGGCCTGAAGCACGTAACTTCTAATGCCAGCGATAGTGAAAGTAGTTACCGTGGTCAAGAAGAATAT GTTTTATCTTATGAGCCAGTGAATCAACAAGAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTA TGAAAGACAGAGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATAC AACTAGACCGAAGCGTGACTATGAGGTGGATGGACGAGATTATCATTTTGTGACTTCAAGGGAACAGATG GAAAAGGATATTCAGGAGCATAAGTTCATTGAAGCCGGCCAGTATAACAACCATCTGTATGGGACGAGCG TGCAGTCCGTGCGAGCAGTGGCAGAGAAGGGCAAACATTGTATCCTTGATGTGTCTGGAAATGCCATAAA GAGGTTGCAGATTGCACAGCTTTATCCAATATCTATTTTTATTAAACCCAAATCCATGGAAAATATCATG GAAATGAACAAGCGCCTAACAGAAGAGCAGGCCAGAAAAACATTTGAGAGAGCCATGAAGCTGGAGCAGG AGTTCACTGAGCATTTCACAGCTATTGTCCAGGGAGACACGCTGGAGGACATTTACAACCAAGTGAAACA GATCATCGAAGAACAGTCTGGGCCTTACATCTGGGTCCCAGCGAAAGAAAAGCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252435
Insert Size	2718 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252435.1, NP_001239364.1
RefSeq Size	4370 bp
RefSeq ORF	2718 bp
Locus ID	13383
UniProt ID	Q811D0
Gene Summary	Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels (By similarity). Functional regulator of Kv1.5 channel (By similarity). May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks an exon and includes an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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