Limch1 (NM_001256122) Mouse Untagged Clone

CAT#: MC229141

Limch1 (untagged) - Mouse LIM and calponin homology domains 1 (Limch1), transcript variant 2



  "NM_001256122" in other vectors (1)

CNY 8,650.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 3732412D22Rik; mKIAA1102
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229141 representing NM_001256122
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCTGAAAGACAGGAGACTGACGACATTGATAGCCCGAAGCGCAGCATCCGAGACAGTGGCTACA
TCGACTGCTGGGACTCTGAACGCAGCGACTCCCTCTCTCCTCCTCGCCACGGACGGGATGACTCCTTTGA
CAGCCTGGATTCTTTTGGCTCCCGCTCCAGGCAGACACCTTCGCCAGATGTAGTTCTCAGGGGAAGCAGT
GATGGAAGAGGAAGCGATTCTGAATCAGACTTGCCGCACCGGAAGTTGCCAGATGTGAAGAAAGATGATA
TGTCCGCACGAAGGACTTCTCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAA
AAGCAACCAGACGGCCTACGTCCCGGCGCCTCTGAGAAAGAAGAAGGCGGAGCGGGAGGAATTCAGGAAG
AGTTGGAGCACCGCCACCTCCCCCCTGGGTGGGGAAAGGCCCTTCAGATACGGTCCCAGAACTCCTGTGT
CAGATGACGCAGAGAGCACGAGTATGTTTGACATGCGGTGTGAGGAGGAGGCAGCGGTGCTGCCGCACAG
CAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGGCAAGAT
GACCTGGCTCGCTGGAAGAGCCGGAGAAGAAGCGCTTCTCAGGACTTGATCAAGAAAGAGGAAGAAAGGA
AAAAAATGGAGAAGCTGATGTCTGGGGAAGATGGGACAAGTGAGCGGAGGAAAAGCATCAAAACTTACAG
AGAAATCGTCCAAGAAAAAGAGCGGAGAGAGAGGGAACTTCATGAGGCGTACAAGAACGCAAGGTCGCAG
GAGGAGGCCGAGGGGATCCTCCAGCAGTACATCGAGAGGTTCACCATCAGTGAGGCTGTCCTCGAACGCT
TGGAGATGCCAAAAATCCTGGAAAGAAGCCATTCAACAGAGCCAAACGTGTCCTCCTTCCCAAACGACCC
CAGCCCCATGAAGTACCTGCGGCAGCAGTCACTGCCCCCACCCAAGTTCACTGCCACGGTTGAGACCACC
ATAGCTCGCACCAGTGTCCCAGAGAGCATTGCGTCAGCAGGCACTGGGTCTCCAAGCAAAATCATCACTC
CAAACACAGTGCCTATGCTGACACCCAGGCCTTACTCCCAGCCCAAAAACTCTCAAGAGGTTCTGAAGAC
TTTTAAGGTAGATGGGAAGGTCAGCATGAATGGGGAAATGGCCCCTGGAGATGAGGAAGGAAAGGAAAAA
GAAGGCCCTGCAGCTGCAGCCCCTGGCCCTTCCTTAACCAAGTCCCAGATGTTTGAAGGTGTGGCCACGG
TGCACGACTCTCCTGTGCAGGTGAAACAAGGCAGCAACAGCATCGAGATCAACATCAAGAAGCCAAATTC
TGCTCCCCAGGAACTGACAGCAGCCTCTGAGGAAACTGAATCGAACGGCCAAGAGGATGAAGACGGTGAA
GAAAGGCCAGGGACAGGGGATTTGGAGCCTGACTCGGCAGAACCCCAGCACTTTACGACAACTGTGACTC
GGTGCAGCCCAACAGTGGCCTTGGTGGAGTTTTCATCCAACCCGCAGCTGAAGAATGAGGTGCCAGAACA
AGGCCAGAAGAAGCCAGAAGATGAGATGAGTGGGAAGGTGGAGTTAGTGTTGTCACAGAAGGTGGCAAAG
CCAAAATCCCCAGAGCCTGAAGCAACCCTGACGTTTCCATTTCTCGACAAAATGCCTGAAACCAACCAAC
TACATTTGCCAAATCCCAGTTCTCAAGCGGATTCTCCAAGCAGTGAAAAGTCCCCTGGGAGCACACCCTT
TAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCCGGCGACAGGAGAAATGGCAGCAGGAGCAGGAACGT
CTGCTGCAGGAGAGGTATCAGAAGGAGCAAGACAAGCTGAAGGAGGAGTGGGAAAAGGCCCAGAAGGAGG
TCGAAGAAGAAGAACGCAGATACTACGAGGAGGAGCGTAAGATAATTGAAGACACGGTGGTTCCATTCAC
TATTTCCTCAAGCTCTGCAGACCAACTGTCTACGTCCTTGTCTGTAACTGAAGGCAGCGGGACAAGGAAT
AAGATGGACTTGGAAAACTGCCCAGACAAAGAAAACGAGAGAAGACAGAAGACGCCTTTCCAGGAGAATG
ACGGTGATTCATTGCTCAAAACTAGAGAAGGTGGTCTGCCAGAGGAGCAGAGCCTAACTCCAAGTCCCTC
GGCTAACCCTGAAATCTCCGTGTCAAAAGGAATCCATCAGGACCCGCAGCTGGAGGCAGAGGCTGGGGCC
CCACACTGTGGTACAAACCCACAGCCAGCTCAGGATCCACCTCGGAATCAACAGATACCAAACCCACCAA
CATCCACGTCGGAAGATGTGAAGCCCAAAACCTTGGCCCTGGAGAAAACCATCAATCATCAGATGGAGTC
TCCGGGGGAAAGGCGGAAGTCTATAAGCGGGAAGAAACTGTGCTCCTCCTGCGGGCTCACTTTGGGTAAA
GGTGCTGCGATGATCATCGAGACCCTGAATCTCTACTTTCACATTCAGTGTTTCAGGTGCGGCATCTGTA
AAGGACAGCTCGGAGATGCAGTAAGCGGGACAGACGTCAGGATTCGCAATGGTCTCCTAAACTGTACCGA
CTGCTACATGCGATCCAGAAGTGCCGGCCAGCCTACAACACTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001256122
Insert Size 2706 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001256122.1, NP_001243051.1
RefSeq Size 5904 bp
RefSeq ORF 2706 bp
Locus ID 77569
UniProt ID Q3UH68
Gene Summary Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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