Limch1 (NM_001256122) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3732412D22Rik; mKIAA1102
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229141 representing NM_001256122 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCTGAAAGACAGGAGACTGACGACATTGATAGCCCGAAGCGCAGCATCCGAGACAGTGGCTACA TCGACTGCTGGGACTCTGAACGCAGCGACTCCCTCTCTCCTCCTCGCCACGGACGGGATGACTCCTTTGA CAGCCTGGATTCTTTTGGCTCCCGCTCCAGGCAGACACCTTCGCCAGATGTAGTTCTCAGGGGAAGCAGT GATGGAAGAGGAAGCGATTCTGAATCAGACTTGCCGCACCGGAAGTTGCCAGATGTGAAGAAAGATGATA TGTCCGCACGAAGGACTTCTCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAA AAGCAACCAGACGGCCTACGTCCCGGCGCCTCTGAGAAAGAAGAAGGCGGAGCGGGAGGAATTCAGGAAG AGTTGGAGCACCGCCACCTCCCCCCTGGGTGGGGAAAGGCCCTTCAGATACGGTCCCAGAACTCCTGTGT CAGATGACGCAGAGAGCACGAGTATGTTTGACATGCGGTGTGAGGAGGAGGCAGCGGTGCTGCCGCACAG CAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGGCAAGAT GACCTGGCTCGCTGGAAGAGCCGGAGAAGAAGCGCTTCTCAGGACTTGATCAAGAAAGAGGAAGAAAGGA AAAAAATGGAGAAGCTGATGTCTGGGGAAGATGGGACAAGTGAGCGGAGGAAAAGCATCAAAACTTACAG AGAAATCGTCCAAGAAAAAGAGCGGAGAGAGAGGGAACTTCATGAGGCGTACAAGAACGCAAGGTCGCAG GAGGAGGCCGAGGGGATCCTCCAGCAGTACATCGAGAGGTTCACCATCAGTGAGGCTGTCCTCGAACGCT TGGAGATGCCAAAAATCCTGGAAAGAAGCCATTCAACAGAGCCAAACGTGTCCTCCTTCCCAAACGACCC CAGCCCCATGAAGTACCTGCGGCAGCAGTCACTGCCCCCACCCAAGTTCACTGCCACGGTTGAGACCACC ATAGCTCGCACCAGTGTCCCAGAGAGCATTGCGTCAGCAGGCACTGGGTCTCCAAGCAAAATCATCACTC CAAACACAGTGCCTATGCTGACACCCAGGCCTTACTCCCAGCCCAAAAACTCTCAAGAGGTTCTGAAGAC TTTTAAGGTAGATGGGAAGGTCAGCATGAATGGGGAAATGGCCCCTGGAGATGAGGAAGGAAAGGAAAAA GAAGGCCCTGCAGCTGCAGCCCCTGGCCCTTCCTTAACCAAGTCCCAGATGTTTGAAGGTGTGGCCACGG TGCACGACTCTCCTGTGCAGGTGAAACAAGGCAGCAACAGCATCGAGATCAACATCAAGAAGCCAAATTC TGCTCCCCAGGAACTGACAGCAGCCTCTGAGGAAACTGAATCGAACGGCCAAGAGGATGAAGACGGTGAA GAAAGGCCAGGGACAGGGGATTTGGAGCCTGACTCGGCAGAACCCCAGCACTTTACGACAACTGTGACTC GGTGCAGCCCAACAGTGGCCTTGGTGGAGTTTTCATCCAACCCGCAGCTGAAGAATGAGGTGCCAGAACA AGGCCAGAAGAAGCCAGAAGATGAGATGAGTGGGAAGGTGGAGTTAGTGTTGTCACAGAAGGTGGCAAAG CCAAAATCCCCAGAGCCTGAAGCAACCCTGACGTTTCCATTTCTCGACAAAATGCCTGAAACCAACCAAC TACATTTGCCAAATCCCAGTTCTCAAGCGGATTCTCCAAGCAGTGAAAAGTCCCCTGGGAGCACACCCTT TAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCCGGCGACAGGAGAAATGGCAGCAGGAGCAGGAACGT CTGCTGCAGGAGAGGTATCAGAAGGAGCAAGACAAGCTGAAGGAGGAGTGGGAAAAGGCCCAGAAGGAGG TCGAAGAAGAAGAACGCAGATACTACGAGGAGGAGCGTAAGATAATTGAAGACACGGTGGTTCCATTCAC TATTTCCTCAAGCTCTGCAGACCAACTGTCTACGTCCTTGTCTGTAACTGAAGGCAGCGGGACAAGGAAT AAGATGGACTTGGAAAACTGCCCAGACAAAGAAAACGAGAGAAGACAGAAGACGCCTTTCCAGGAGAATG ACGGTGATTCATTGCTCAAAACTAGAGAAGGTGGTCTGCCAGAGGAGCAGAGCCTAACTCCAAGTCCCTC GGCTAACCCTGAAATCTCCGTGTCAAAAGGAATCCATCAGGACCCGCAGCTGGAGGCAGAGGCTGGGGCC CCACACTGTGGTACAAACCCACAGCCAGCTCAGGATCCACCTCGGAATCAACAGATACCAAACCCACCAA CATCCACGTCGGAAGATGTGAAGCCCAAAACCTTGGCCCTGGAGAAAACCATCAATCATCAGATGGAGTC TCCGGGGGAAAGGCGGAAGTCTATAAGCGGGAAGAAACTGTGCTCCTCCTGCGGGCTCACTTTGGGTAAA GGTGCTGCGATGATCATCGAGACCCTGAATCTCTACTTTCACATTCAGTGTTTCAGGTGCGGCATCTGTA AAGGACAGCTCGGAGATGCAGTAAGCGGGACAGACGTCAGGATTCGCAATGGTCTCCTAAACTGTACCGA CTGCTACATGCGATCCAGAAGTGCCGGCCAGCCTACAACACTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256122
Insert Size	2706 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256122.1, NP_001243051.1
RefSeq Size	5904 bp
RefSeq ORF	2706 bp
Locus ID	77569
UniProt ID	Q3UH68
Gene Summary	Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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