Taok3 (NM_001199685) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900006A08Rik; A130052D22; A430105I05Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229137 representing NM_001199685 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTAAAGGGGCACTGAAGGACCCAGAGATTGCTGATCTGTTCTTCAAGGATGACCCCGAAGAGCTTT TTATCGATTTGCATGAAATTGGACATGGGAGCTTTGGAGCCGTGTACTTTGCGACAAATGCGCACACCAA CGAGGTGGTCGCTGTTAAGAAAATGTCCTACAGTGGGAAGCAGACCCATGAGAAATGGCAAGATATCCTT AAGGAAGTTAAGTTTCTGCAGCAGCTGAAGCATCCTAACACCATCGAGTACAAAGGCTGTTATTTAAAGG AGCACACTGCATGGTTGGTGATGGAGTACTGCCTAGGTTCAGCCTCTGACTTGTTAGAAGTTCATAAGAA ACCACTTCAGGAAGTGGAGATCGCTGCTATCACCCATGGTGCCTTGCAGGGGCTGGCCTACCTCCACTTT CACTCCCTGATTCACAGGGACATTAAAGCAGGGAACATCCTCCTCACAGAGCCAGGTCAGGTGAAGCTGG CTGACTTCGGATCTGCCTCCATGGCTTCTCCCGCCAACTCCTTTGTGGGGACTCCTTACTGGATGGCCCC AGAGGTAATCTTAGCTATGGACGAAGGGCAGTACGACGGGAAAGTTGACATCTGGTCGCTTGGCATCACC TGCATAGAGTTGGCGGAGCGGAAGCCCCCCCTCTTCAACATGAACGCCATGAGCGCCCTCTACCACATCG CTCAGAATGACTCCCCCACGCTACAGTCCAGGGAATGGACAGACTCCTTCAGGAGATTCGTTGATTACTG CTTGCACAAAATACCTCAGGAAAGACCAGCCGCGGTGGAGCTGTTGCGGCATGACTTCATTCGGAGGGAG CGGCCACCGAAGGTCCTCATTGATCTCATACAGAGGACAAAAGATGCTGTCCGAGAGCTGGACAACCTGC AGTACAGGAAAATGAAGAAGATCCTCTTCCAGGAGACTCGGAACGGACCCTTGAATGAGTCGCAAGAAGA AGAAGAAGATGGTGAGCAAGGAAGCAACCTGAACCGAGAGGTGGACAGTCTGGGCAGCATCCACTCCATT CCCAGCACATCAGTGAGCACAGGCAGCCGGAGCAGCAGTGTGAACAGCATGCAGGAGGTCATGGATGAGA GCAGCTCTGAGCTCGTCATGATGCAGGAGGATGAAGGCACAGCCAACTCCAGCGCCTCCACGGTACACAA GAAGGATCATGTATTCGTAAGGGATGAGGCGGGCCACGGCGATCCCAGGCCTGAGCCGCGGCCTACCCAG TCAGTTCAGAGCCGGGCCCTCCACTATCGGAACCGAGAGCGCTTTGCCACGATCAAATCCGCGTCTTTGG TTACACGGCAGATCCATGAGCATGAGCAGGAGAACGAGCTGCGGGAACAGATGTCAGGTTATAAGCGGAT GCGGCGCCAGCACCAGAAGCAGCTGATCGCCCTGGAGAACAAGCTGAAGGCTGAGATGGATGAGCACCGC CTCAAGCTCCAGAAGGAGGTGGAGACGCACGCCAACAACTCGTCCATCGAGCTGGAGAAGCTGGCCAAGA AGCAAGTGGCTACCATAGAAAAGGAGGCAAAGGTAGCCGCAGCGGATGAGAAGAAGTTCCAGCAGCAGAT CCTGGCCCAGCAGAAGAAGGACTTGACAACTTTCTTAGAAAGTCAGAAGAAGCAGTACAAGATTTGTAAG GAGAAAATAAAAGAGGAAATGAACGAGGACCATAGCACACCCAAGAAGGAGAAGCAGGAGCGGATATCGA AGCATAAAGAAAACCTGCAGCACACGCAGGCCGAGGAGGAAGCCCACCTGCTCACTCAACAGAGGCTGTA CTACGACAGAAACTGCCGCTTCTTCAAACGGAAAATAATGATCAAGCGCCACGAGGTGGAACAGCAGAAC ATTCGGGAGGAATTAAATAAGAAGAGGACCCAGAAGGAAATGGAGCACGCCATGCTGATCCGGCATGATG AATCCACCCGAGAGCTGGAGTACAGGCAGCTGCACACCCTGCAGAAGCTCCGCATGGATCTGATCCGGCT GCAGCACCAGACGGAACTGGAGAACCAGCTGGAGTACAATAAGAGGCGGGAGCGGGAGCTGCACCGGAAG CACGTCATGGAGCTTCGGCAACAGCCGAAAAACTTAAAGGCCATGGAAATGCAAATTAAAAAACAGTTTC AGGACACTTGCAAAGTACAGACCAAACAGTACAAAGCCCTCAAAAATCACCAGTTGGAAGTCACTCCAAA GAATGAGCACAAAGCAATCCTAAAGACACTGAAGGACGAGCAGACGAGGAAGCTGGCCATTTTGGCCGAG CAGTATGAACAGAGTATAAACGAGATGATGGCCTCTCAAGCGTTACGGCTAGACGAGGCACAAGAGGCAG AGTGCCAGGCCTTGAGACTACAGCTCCAGCAGGAGATGGAGCTGCTCAACGCCTACCAGAGCAAAATCAA GATGCAAACGGAGGCCCAGCACGAGCGCGAGCTACAGAAGCTAGAGCAGAGGGTGTCCCTGCGCAGAGCG CATCTTGAGCAGAAGATTGAAGAGGAGCTGGCTGCCCTGCAGAAGGAACGCAGTGAGAGGATCAAGACTC TCCTGGAGAGACAAGAGCGAGAGACCGAGACTTTTGACATGGAGAGCCTCAGAATGGGCTTTGGGAATTT GGTGACATTAGATTTTCCTAAGGAGGACTATAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199685
Insert Size	2697 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199685.1, NP_001186614.1
RefSeq Size	4366 bp
RefSeq ORF	2697 bp
Locus ID	330177
UniProt ID	Q8BYC6
Gene Summary	Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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