Rbbp8 (NM_001252495) Mouse Untagged Clone

CAT#: MC229119

Rbbp8 (untagged) - Mouse retinoblastoma binding protein 8 (Rbbp8), transcript variant 2



  "NM_001252495" in other vectors (1)

CNY 8,550.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9930104E21Rik; CtIP; RBBP-8; RIM; SAE2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229119 representing NM_001252495
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCATTTCAGGAAGCGGCTGTGGAAGCCCTAATTCTGCGGATGCATCCAATGACTTCAAGGAACTCT
GGACAAAACTAAAAGAGTATCATGATAAGGAAGTGCAAGGTTTACAAGTCAAAGTAACCAAACTGAAAAA
GGAACGAATTTTAGATGCACAGAGACTAGAAGAATTCTTCACCAAAAATCAACAGCTGAGAGACCAGCAG
AAAGTCCTTCAGGAAACCATTAAAATTTTAGAAGATAGGTTAAGAGCAGGATTGTGTGATCGCTGTGCAG
TAACTGAAGAGCATATGCACAAAAAGCAGCAGGAGTTTGAAAATATCCGACAGCAGAACCTTAAGCTTAT
TACAGAGCTGATGAATGAAAAGAATACTCTTCAAGAAGAAAACAAAAAGCTTTCTGAACAACTGCAGCAG
AAAATGGAAAATGGTCAACAGGATCAAGTAGCTGAGCTTGCATGTGAGGAAAACATTATTCCAGACTCAC
CAGTAACATCCTTTTCCTTTTCTGGCATTAACCGGCTACGAAAGAAGGAGAATCTCCATGTCCGATACGT
GGAACAAACACATACTAAGTTGGAGCGCTCTCTATGTACAAATGAATTAAGAAAAATATCTAAGGATTCA
GCTCCTGCACCAGTTAACTCTGAGGAACATGAAATTCTCGTAGCTGACACTTGTGATCAAAATCACTCAC
CACTGTCAAAAATATGTGAAACAAGCAGTTACCCCACTGATAAGACATCTTTTAATTTAGACACTGTTGT
TGCTGAGACACTTGGACTTAATGGTCAAGAAGAATCTGAACCCCAAGGTCCCATGAGCCCTCTGGGAAGT
GAACTCTACCACTGTCTCAAAGAAGATCACAAAAAACACCCTTTTATGGAATCTGCAAGAAGTAAGGAAG
ATAGCTTAAGATTTTCAGACTCTGCTTCAAAGACACCTCCTCAAGAATTTACTACTCGGGCGTCATCTCC
GGTATTTGGAGCTACCTCTACTGTGAAAGCTCATTTGGGTTTGAATACAAGTTTCTCCCCCTCTCTCTTA
GACATTGGGAAAAAAAACCTTCTGAAGACAGCTCCCTTTAGCAACATTGCTGTTTCTAGATCAGAGAAAG
TTAGATCCAAATCTGAGGATAATGCCCTTTTCACACAACATAGTCTTGGGTCAGAAGTGAAGGTCATTAG
CCAGTCATTTTCTAGTAAACAGATACTTACAAATAAAACTGTAAGTGACTCTGTAGATGAACAGTGCAGT
GCTGACCACATGAATACTACTGTTGCTGATAAATATTTGGTGCCTTTGAAATCCCTGGGAGGCAAAGCAT
CCAAAAGGAAGAGGACTGAGGAGGAAAGTGAGCATGCAGTAAAGTGCCCCCAGGCCTGTTTTGATAAGGA
AAATGCCCTTCCTTTCCCAATGGAGAATCAGTTCTCCATGAATGGAGACCATGTGATGGATAAACCTCTG
GACCTGTCAGATCGGTTTGCAGCTACTCAGCGGCAAGAGAAGAACCACGGAAACGAGACTTCTAAAAACA
AACTTAAGCAAGCCACTATTTACGAGGCTTTGAAGCCCATTCCAAAGGGCTCCTCCTCAGGCCGTAAAGC
CTTGAGTGGGGACTGCATGCCAGCCAAAGACTCTTGGGAGACATACTGCTTACAGCCGAGGAGCCTCCAG
TCCTCTAGTAAATTCTCTCCAGACCAAAAAACACCATTACAGATAAAAGAAGAAAATCCTGTCTTCAAAA
CACCTCCATGTTCACAGGAAAGTTTGGAGACAGAGAATCTCTTTGGTGATGTGAAGGGTACTGGTTCTCT
TGTGCCAACAAAAGTAAAGAGCAGAGCAGTCCATGGAGGATGTGAGCTTGCATCAGTTCTTCAGTTAAAC
CCCTGTAGAGTTGCTAAAACAAAGGCTCTGCCAAGCAACCAAGATACGTCCTTTGAAAATATCCAGTGGA
GTGTAGACCCAGGAGCAGACCTTTCTCAGTATAAAATGGATGTTACTGTAATAGATACAAAGGATAGCAG
TCACTCACGATTAGGAGGAGAAACAGTGGACATGGACTGTACATTGGTCAGTGAAACCGTGCTTTTAAAA
ATGAAGAAACAAGAGCAAAAAGAAAGAAGTCCAAATGGAGACATAAAAATGAATGATAGCCTAGAAGATA
TGTTTGATCGGACAACACATGAAGAGTATGAGTCCTGTTTGGCAGATAGCTTCTCCCAGGTACCAGATGA
GGAAGAACTGCCTGACACCACAAAGAAAACAAACATACCTGCTGATAAACAAGATGGAGTCAAACAGAAA
GCATTTGTGGGGCCATATTTTAAAGATAAGGAGAGAGAAACTAGCATACAGAATTTTCCTCATATTGAGG
TAGTTCGGAAAAAAGAAGAGAGAAGGAAATTGCTTGGGCACACATGTAAAGAATGTGAAATTTATTATGC
AGATTTGCCAGCAGAAGAAAGAGAAAAGAAGTTGGCTTCCTGCTCAAGACACCGATTTCGCTACATTCCA
CCCAACACACCAGAGAATTTCTGGGAAGTTGGTTTTCCTTCTACTCAGACGTGTCTGGAAAGAGGTTATA
TCAAGGAAGATCTCGATCTTAGTCCTCGTCCCAAAAGACGGCAGCCTTACAACGCGGTCTTTTCTCCAAA
AGGCAAAGAACAGAGGACCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001252495
Insert Size 2682 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001252495.1, NP_001239424.1
RefSeq Size 3523 bp
RefSeq ORF 2682 bp
Locus ID 225182
UniProt ID Q80YR6
Gene Summary Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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