Trpc4 (NM_001253682) Mouse Untagged Clone

CAT#: MC229112

Trpc4 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 (Trpc4), transcript variant 2



  "NM_001253682" in other vectors (1)

CNY 8,550.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-TRPC4
    • 50 ul

CNY 11,000.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CCE1; STRPC4; Trp4; Trrp4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229112 representing NM_001253682
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGTTCTATTACAAAAGAAATGTCAACGCCCCCTACAGAGACCGCATCCCACTGAGGATTGTCA
GAGCAGAATCTGAGCTCTCACCATCAGAGAAAGCCTACTTGAATGCTGTGGAGAAGGGGGACTATGCAAG
CGTCAAGAAGTCTCTGGAGGAAGCTGAGATTTATTTTAAAATCAACATTAACTGCATCGACCCCCTGGGA
AGGACCGCCCTCCTCATTGCCATTGAAAATGAGAATCTGGAGCTTATTGAACTATTGTTGAGTTTCAATG
TCTATGTAGGCGATGCGCTGCTTCACGCCATCAGAAAAGAGGTGGTTGGAGCCGTGGAGCTACTGCTGAA
CCACAAAAAGCCAAGTGGAGAGAAGCAGGTGCCTCCCATTCTCCTTGATAAACAGTTCTCTGAATTCACT
CCGGACATCACACCCATCATCTTGGCTGCACATACAAATAATTACGAGATAATCAAACTTTTGGTTCAGA
AAGGTGTCTCAGTGCCCAGACCCCACGAGGTCCGCTGTAACTGTGTTGAGTGTGTCTCCAGCTCGGATGT
GGACAGCCTCAGGCATTCACGGTCCAGGCTCAACATCTACAAGGCCTTGGCCAGCCCCTCGCTCATTGCC
CTGTCAAGCGAAGACCCTTTCCTTACTGCCTTTCAGTTAAGTTGGGAGCTGCAAGAACTCAGCAAGGTGG
AGAACGAATTCAAGTCGGAGTATGAGGAGCTGTCTAGACAGTGCAAACAATTTGCCAAGGACCTCCTAGA
TCAGACACGGAGTTCCAGAGAGCTGGAAATCATTCTTAATTACCGTGATGACAATAGTCTGATCGAAGAA
CAGAGTGGAAATGATCTTGCAAGGCTAAAATTAGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCTCAGC
CCAACTGCCAGCAGCTGCTCGCTTCCCGCTGGTACGATGAGTTCCCAGGCTGGAGGAGAAGACACTGGGC
GGTGAAGATGGTGACGTGTTTCATAATAGGACTACTCTTCCCCGTCTTCTCCGTGTGCTACCTGATAGCT
CCCAAAAGCCCACTTGGACTGTTCATCAGAAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATC
TGACCTTTTTGTTTCTGCTGCTGCTAGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC
ACCACCAACCATCGTGGAGTGGATGATATTACCGTGGGTCCTGGGTTTTATATGGGGAGAGATTAAACAG
ATGTGGGATGGCGGACTCCAGGATTACATCCATGACTGGTGGAATCTAATGGACTTTGTGATGAACTCCT
TGTATCTGGCAACAATCTCCTTGAAGATTGTCGCGTTTGTAAAGTACAGTGCTCTGAACCCACGGGAATC
ATGGGACATGTGGCACCCCACCCTGGTGGCAGAGGCATTATTTGCTATTGCAAACATCTTCAGTTCCCTC
CGCCTGATCTCTCTGTTCACTGCCAATTCTCACCTGGGGCCTCTGCAGATATCTCTGGGAAGGATGCTTC
TGGACATCCTGAAGTTCTTGTTCATCTACTGCCTCGTGCTGCTAGCTTTTGCAAATGGCCTAAATCAGCT
GTACTTTTACTATGAAGAAACAAAGGGGCTAAGCTGCAAAGGCATCCGGTGCGAGAAACAGAACAACGCG
TTTTCCACGTTATTCGAGACACTACAGTCCCTGTTTTGGTCAATATTTGGACTCATCAATCTCTATGTTA
CCAATGTCAAAGCCCAGCACGAGTTCACTGAGTTTGTTGGGGCCACCATGTTTGGCACATATAATGTCAT
CTCTCTGGTTGTCCTGCTGAACATGTTAATTGCTATGATGAATAATTCTTACCAACTAATTGCCGACCAT
GCAGATATAGAATGGAAATTTGCTCGAACAAAGCTTTGGATGAGCTACTTTGAAGAAGGAGGTACCCTGC
CTACACCTTTCAATGTCATCCCAAGCCCCAAGTCCCTGTGGTACCTGGTCAAGTGGATATGGACACACTT
ATGTAAGAAAAAAATGAGAAGGAAGCCAGAAAGCTTCGGGACAATTGGGCGGCGTGCTGCTGATAACTTG
AGAAGACATCACCAATACCAAGAGGTGATGAGGAACCTGGTGAAGCGGTACGTGGCAGCCATGATCAGAG
AGGCAAAAACCGAAGAAGGCTTGACGGAGGAGAATGTTAAGGAACTAAAGCAAGACATTTCTAGCTTCCG
CTTCGAAGTTCTGGGATTGCTCAGAGGAAGCAAGCTCTCTACAATACAGTCAGCCAACGCGGCGAGTTCA
GCGGACTCCGACGAGAAGAGCCAGAGCGAAGAAGAAATTACTCGTCAACAGGCGGCAGGAGCACTTGAGC
GAAATATCGAACTGGAATCCAAAGGATTAGCTTCACGGGGTGACCGCAGCATTCCTGGTCTCAATGAACA
GTGTGTGCTAGTAGACCATAGAGAAAGGAATACGGACACTTTGGGTTTACAGGTAGGCAAGAGAGTGTGC
TCCACCTTCAAGTCGGAGAAGGTGGTGGTGGAAGACACCGTCCCTATTATACCAAAGGAGAAACACGCCC
ACGAGGAGGACTCGAGCATAGACTATGACTTAAGCCCCACGGACACAGCTGCCCACGAAGATTATGTGAC
CACAAGATTGTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001253682
Insert Size 2673 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001253682.1, NP_001240611.1
RefSeq Size 3238 bp
RefSeq ORF 2673 bp
Locus ID 22066
UniProt ID Q9QUQ5
Gene Summary Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame coding segment in the last exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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