Trpc4 (NM_001253682) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CCE1; STRPC4; Trp4; Trrp4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229112 representing NM_001253682 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTCTATTACAAAAGAAATGTCAACGCCCCCTACAGAGACCGCATCCCACTGAGGATTGTCA GAGCAGAATCTGAGCTCTCACCATCAGAGAAAGCCTACTTGAATGCTGTGGAGAAGGGGGACTATGCAAG CGTCAAGAAGTCTCTGGAGGAAGCTGAGATTTATTTTAAAATCAACATTAACTGCATCGACCCCCTGGGA AGGACCGCCCTCCTCATTGCCATTGAAAATGAGAATCTGGAGCTTATTGAACTATTGTTGAGTTTCAATG TCTATGTAGGCGATGCGCTGCTTCACGCCATCAGAAAAGAGGTGGTTGGAGCCGTGGAGCTACTGCTGAA CCACAAAAAGCCAAGTGGAGAGAAGCAGGTGCCTCCCATTCTCCTTGATAAACAGTTCTCTGAATTCACT CCGGACATCACACCCATCATCTTGGCTGCACATACAAATAATTACGAGATAATCAAACTTTTGGTTCAGA AAGGTGTCTCAGTGCCCAGACCCCACGAGGTCCGCTGTAACTGTGTTGAGTGTGTCTCCAGCTCGGATGT GGACAGCCTCAGGCATTCACGGTCCAGGCTCAACATCTACAAGGCCTTGGCCAGCCCCTCGCTCATTGCC CTGTCAAGCGAAGACCCTTTCCTTACTGCCTTTCAGTTAAGTTGGGAGCTGCAAGAACTCAGCAAGGTGG AGAACGAATTCAAGTCGGAGTATGAGGAGCTGTCTAGACAGTGCAAACAATTTGCCAAGGACCTCCTAGA TCAGACACGGAGTTCCAGAGAGCTGGAAATCATTCTTAATTACCGTGATGACAATAGTCTGATCGAAGAA CAGAGTGGAAATGATCTTGCAAGGCTAAAATTAGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCTCAGC CCAACTGCCAGCAGCTGCTCGCTTCCCGCTGGTACGATGAGTTCCCAGGCTGGAGGAGAAGACACTGGGC GGTGAAGATGGTGACGTGTTTCATAATAGGACTACTCTTCCCCGTCTTCTCCGTGTGCTACCTGATAGCT CCCAAAAGCCCACTTGGACTGTTCATCAGAAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATC TGACCTTTTTGTTTCTGCTGCTGCTAGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC ACCACCAACCATCGTGGAGTGGATGATATTACCGTGGGTCCTGGGTTTTATATGGGGAGAGATTAAACAG ATGTGGGATGGCGGACTCCAGGATTACATCCATGACTGGTGGAATCTAATGGACTTTGTGATGAACTCCT TGTATCTGGCAACAATCTCCTTGAAGATTGTCGCGTTTGTAAAGTACAGTGCTCTGAACCCACGGGAATC ATGGGACATGTGGCACCCCACCCTGGTGGCAGAGGCATTATTTGCTATTGCAAACATCTTCAGTTCCCTC CGCCTGATCTCTCTGTTCACTGCCAATTCTCACCTGGGGCCTCTGCAGATATCTCTGGGAAGGATGCTTC TGGACATCCTGAAGTTCTTGTTCATCTACTGCCTCGTGCTGCTAGCTTTTGCAAATGGCCTAAATCAGCT GTACTTTTACTATGAAGAAACAAAGGGGCTAAGCTGCAAAGGCATCCGGTGCGAGAAACAGAACAACGCG TTTTCCACGTTATTCGAGACACTACAGTCCCTGTTTTGGTCAATATTTGGACTCATCAATCTCTATGTTA CCAATGTCAAAGCCCAGCACGAGTTCACTGAGTTTGTTGGGGCCACCATGTTTGGCACATATAATGTCAT CTCTCTGGTTGTCCTGCTGAACATGTTAATTGCTATGATGAATAATTCTTACCAACTAATTGCCGACCAT GCAGATATAGAATGGAAATTTGCTCGAACAAAGCTTTGGATGAGCTACTTTGAAGAAGGAGGTACCCTGC CTACACCTTTCAATGTCATCCCAAGCCCCAAGTCCCTGTGGTACCTGGTCAAGTGGATATGGACACACTT ATGTAAGAAAAAAATGAGAAGGAAGCCAGAAAGCTTCGGGACAATTGGGCGGCGTGCTGCTGATAACTTG AGAAGACATCACCAATACCAAGAGGTGATGAGGAACCTGGTGAAGCGGTACGTGGCAGCCATGATCAGAG AGGCAAAAACCGAAGAAGGCTTGACGGAGGAGAATGTTAAGGAACTAAAGCAAGACATTTCTAGCTTCCG CTTCGAAGTTCTGGGATTGCTCAGAGGAAGCAAGCTCTCTACAATACAGTCAGCCAACGCGGCGAGTTCA GCGGACTCCGACGAGAAGAGCCAGAGCGAAGAAGAAATTACTCGTCAACAGGCGGCAGGAGCACTTGAGC GAAATATCGAACTGGAATCCAAAGGATTAGCTTCACGGGGTGACCGCAGCATTCCTGGTCTCAATGAACA GTGTGTGCTAGTAGACCATAGAGAAAGGAATACGGACACTTTGGGTTTACAGGTAGGCAAGAGAGTGTGC TCCACCTTCAAGTCGGAGAAGGTGGTGGTGGAAGACACCGTCCCTATTATACCAAAGGAGAAACACGCCC ACGAGGAGGACTCGAGCATAGACTATGACTTAAGCCCCACGGACACAGCTGCCCACGAAGATTATGTGAC CACAAGATTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001253682
Insert Size	2673 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253682.1, NP_001240611.1
RefSeq Size	3238 bp
RefSeq ORF	2673 bp
Locus ID	22066
UniProt ID	Q9QUQ5
Gene Summary	Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame coding segment in the last exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

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