Uspl1 (NM_001286825) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E430026A01Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229111 representing NM_001286825 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTAAGAGCAGATGTTTACCACTTTGCCAGACTCTGTGTGTCCAGTGGAAAAACACCCAGGCCC TGTGCTGGTTAGACTGTATCCTGTCAGCCCTGGTGCACTTGGAGGTGTTGAGGAAGACTGTGCTGGAGGC GTGCTCCAGGGAGGAGTGTGTATTTGGAAGGCTGTTTGAAATGTACCACCAAGCAGATGAGCTGCTGCAC ACCCATCACCTGCATGGCGTCACAGGTGAAGATTGTAAAAAATTGACATCAGAAATATTTACAGAAATAG ATACCTGTTTGAATAAAGTTAGAGATGAAATTTTTGCTAAACTTCAACCGAAGCTTAGATGCACATTAGG TGACATGGAAAGTCCTGTGTTTGCACTTCCTGTACTGTTAAAGCTTGAACCCCATGTTGAAAGCCTCTTT ACATATTCTTTTTCTTGGAATTTTGAATGTTCCCATTGTGGACACCAGTACCAAAACAGGTGTGTGAAGA GTCTGGTCACCTTTACCAATATTGTTCCTGAGTGGCATCCACTCAATGCTGCCCATTTTGGTCCATGTAA CAGCTGCAACAGTAAATCACAAATAAGAAAAATGGTGTTGGAAAGAGCGTCGCCCATACTCATGCTGCAC TTCGTGGAGGGCTTACCAAGGAGGGATTTGCAACACTATGCCTTCCACTTTGAAGGAAGCCTTTATCAAG TCACGTCGGTAATACAGTACCAAGCAAATAACCATTTTATAACATGGTTTTTAGATGCTGATGGGAGTTG GCTGGAGTGTGATGACCTAAAGGGTCCCTGTGCTAAAAGGCACGTGACCTGTGAAGTGCCAGCTTCAGAG ACACATATCGTTATTTGGGAAAGAAAATCCCAGGTGCCAATTGAAGAAGCTGCCTGCCTTCCATGCATGA AGCCAAATGTGCAGCCTGTATCAGGTGAGGAGCAGCCGACTTGTCCAGCACTGTGTTCTCTGGCTGGGAC CGCCACCTCAGAACCCTCAGTCGCCCATCCCACATCTATGGCAGGCGCTCCTCAGACTCTTCCAGAAATC CAAGCTGTAGCTCATGGAGATAGTGTACTTTCAGGAGCAAAAGGCATGGTCGACAGCATTTTACCCTCAG CACTTGAAGAAACCATCCAGGAAACTGCCTCTGTTTCTCAGGTTGACTCCAAAGATTGCCTGTTGGAAGA CAAACCCGTGGCAGGAAGTGCAGCACTGGTCAGAGTACTTGCTTTTCAGCCACAGGACTCCCCGGGGTCT TCGGGGTCTTCCTTAGTGTCCAGCCTGTGTGAAGGAAAGCTAGTTGCCCCATGTGTGGATTCAAGTTTCC CATCCCAAGCTGTGAGCACAGACCTGCAGGCAGTACTGTCCCAGGCAGGGGACACTGTGGTTCCTAACCC TGTGACTGATGCCCCTGTTCCTGTTCTTGTTCAGGAATTGAAGTCCCTGGCAACTGAGAAGGACTCTCAG ACACAGTTGCTGCCACTGAAAACTGAGAAGTTAGATCCTGAGCAACCTGGTAAATCTCAGGCATCTAATT TGAGAAAACGAGAAACCACAGCGTCTTCTAAAACAGTAGCAGCCAGGTCAGCACAGAATCAGCCTCGGAA AGAAGATCAGAAGAGAGCCTTCGTGGGAAGTTGGGTGAAGGGGCTATTGAGCAGGGGTGGTGCTTTTATG CCAACCTGTGTTTTGTCTCAGAGTAGAGCAGTAAGTGACCTGCAACCTTCTGTTAAAGGAGCCAGTAATT TTGATGGCTTTAAAACGAAAAGTATAAGCCGCAGGTCTAAAAGAATGTCCAGGAAAGCTAAGCACATGGA GGAACTGTCTCCAAGGAACAGCTCTCCACCATTGAGCTGGACAGCTGCTCTGACCCAGGCTGCTGAGAAC GCCACCTCAGCTCTGTTGAGGGAGCAGGAAGGCTCACGTCCAGCTCCCCTCAGGCACAGATCTCCTGGCA ATGAAAGCGCCATCTCTCCAGCAAGCCGTGGAGATGCGGCTGAAGATCAGGTTCATAAGCTTCGGCTCAA ACTTCTTAAAAAATTAAAGGCAAAAAAGAAGAAACTAGCTGCCCTCATTTCTTCCCCGCACCGTGAACCA TCTCTAAGTGACCACTCAGAGCCTGCGTCCCATTGTGGAACACCTGCAAGTGACCAGTCAGAGCCTGTAT CCCATTGTGGATCTCCAAATGACTGTGAGTCCATAGAAGATCTGTTAAAAGAGCTACAGCATCAAATTGA CCTTGCAGACAGCAAATCTGGGTGCACCTCTGCTCCTGATGCCACCTCAAATAACAGCCAGAGTCATGAG GAGATTTTAGCAGAGTTGCTGTCGCCTACAGCCATGTCAGAGCCCTCAGAAAGTGGGGAGCTTGAGTTGA GGTACTTGGAAATGGGGGACAGCACCCCAGCACAAGCACCTAGTGAATTCAGTGTTGTCTCCCAGAACAC ATGTCTGAAACAGGACCATGATTACTGCAGCCCTGAGAAAGGCCAGCGGGAAGTTGATCTGCATTCAGTC ATGGACAGTGCCTGTATAAGAACCTTGAACTTGGGGAGTCCCATGAAGACTGATATCTTTGATGATTTTT TTTCCACCTCAGCACTGAATTCTTTAACAAACGACACATTAGACATACCTCATTTTGATGACTCTCTGTT TGAGAATTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286825
Insert Size	2673 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286825.1, NP_001273754.1
RefSeq Size	3714 bp
RefSeq ORF	2673 bp
Locus ID	231915
UniProt ID	Q3ULM6
Gene Summary	SUMO-specific isopeptidase involved in protein desumoylation. Specifically binds SUMO proteins with a higher affinity for SUMO2 and SUMO3 which it cleaves more efficiently. Also able to process full-length SUMO proteins to their mature forms (By similarity). Plays a key role in RNA polymerase-II-mediated snRNA transcription in the Cajal bodies (By similarity). Is a component of complexes that can bind to U snRNA genes (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (7) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. Variants 3, 7, and 8 all encode the same isoform (B) which is shorter at the N-terminus, compared to isoform A.

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