Bcar1 (NM_001198839) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI385681; Cas; Crkas
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229093 representing NM_001198839 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTACCTGAACGTGCTGGCCAAAGCCCTCTATGACAATGTTGCTGAATCCCCGGATGAGCTCTCCT TCCGAAAGGGTGACATCATGACAGTGCTCGAGCGGGACACTCAGGGCCTGGATGGCTGGTGGCTCTGCTC ACTGCATGGGCGCCAGGGCATTGTGCCTGGTAACCGCCTCAAGATTCTGGTTGGCATGTATGATAAGAAG CCAGTAGGACCTGGACCAGGCCCACCTGCCACCCCACCCCAGCCCCAGCCCAGCCTTCCCCAGGGCGTCC ATGCTCCGGTACCTCCAGCTTCCCAGTACAGTCCCATGCTCCCCACTGCGTACCAGCCCCAGTCTGACAA TGTGTACCTGGTACCCACTCCCAGCAAGACTCAGCAAGGTCTCTACCAAGCCCCTGGGCCCAACCCACAG TTCCAGTCACCCCCAGCCAAACAGACGTCCACATTCTCCAAGCAAACACCTCATCACTCATTTCCCAGCC CAGCCACGGACCTTTACCAGGTGCCCCCAGGGCCTGGAAGTCCAGCCCAGGACATTTATCAGGTGCCACC TTCCGCTGGCATAGGGCATGACATCTACCAAGTTCCTCCATCTCTGGACACTCGCGGATGGGAGGGAACA AAGCCACCGGCAAAGGTGGTGGTTCCTACGCGAGTGGGACAGGGCTATGTGTATGAGGCCGCTCAGACGG AGCAGGATGAGTACGACACCCCACGCCACCTGCTGGCCCCAGGTCCCCAGGACATCTATGATGTGCCCCC TGTTCGAGGACTGCTTCCTAACCAGTATGGCCAGGAGGTATATGACACGCCCCCTATGGCAGTCAAAGGC CCCAATGGCCGAGACCCATTGTTGGATGTGTATGACGTGCCCCCTAGTGTGGAGAAAGGCCTGCTCTCGT CCAGCCATCATTCGGTGTATGATGTTCCTCCTTCTGTGAGCAAGGATGTGCCCGATGGCCCACTGCTGCG TGAGGAAACCTATGATGTACCTCCTGCCTTCGCCAAGCCCAAGCCCTTTGATCCCACCCGACACCCACTG ATCCTTGCTGCGCCTCCTCCCGACTCCCCAGCAGCTGAGGATGTATATGATGTGCCCCCCCCTGCTCCCG ACCTCTATGATGTGCCCCCTGGTTTGCGGCGGCCTGGCCCTGGAACACTGTATGACGTGCCTCGCGAGCG CGTGCTCCCTCCTGAGGTGGCTGATGGGAGTGTAGTTGACGATGGTGTGTATGCTGTGCCCCCACCAGCC GAGCGAGAGGCCCCAACGGATGGCAAGCGCCTGTCGGCCTCTAGCACGGGCAGCACGCGCAGCAGCCAAT CGGCATCTTCCTTGGAGGTGGTGGTGCCAGGCCGGGAGCCCCTGGAACTGGAGGTTGCTGTGGAGTCCCT GGCTCGGCTGCAGCAAGGCGTGAGCACCACCGTAGCCCACCTTCTGGACCTGGTGGGCAGTGCCAGTGGG CCTGGGGGCTGGCGTGGTACCTCTGAGCCTCAGGAGCCTCCCGCACAGGACCTGAAAGCCGCAGTGGCGG CAGTTCACGGGGCAGTCCATGAGCTCCTGGAGTTCGCCCGCGGAGCTGTGAGCAATGCCACCCACACTTC TGACCGCACCTTGCATGCCAAGCTTAGCCGGCAACTACAGAAGATGGAGGATGTGTACCAGACACTGGTG GTCCACGGTCAGGTCCTTGACAGTGGCCGAGGAAGTCCAGGATTCACTCCGGAAGACCTGGACCGCCTGG TGGCCTGCTCTCGGGCTGTGCCCGAGGATGCCAAGCAGCTAGCTTCCTTTTTGCATGGCAATGCCTCGCT GCTTTTCAGACGGACCAAAGCCCCTGGCCCAGGGCCTGAGGGAAGCAGCTCCCTGCACCCAAACCCCACT GATAAAGCCAGTAGCATCCAGTCACGCCCTCTCCCCTCACCTCCAAAGTTCACCTCCCAGGACTCTCCAG ACGGCCAGTATGAGAACAGTGAAGGGGGTTGGATGGAGGACTATGACTACGTCCATCTGCAGGGGAAGGA GGAATTTGAGAAGACCCAGAAGGAGCTGCTAGAAAGGGGTAACATCATGCGGCAGGGAAAGGGCCAACTG GAGTTGCAGCAGCTGAAACAGTTTGAGCGACTGGAGCAGGAGGTGTCTCGTCCAATAGACCACGACCTGG CCAACTGGACACCAGCCCAGCCCCTGGTGCCGGGCCGGACAGGGGGCCTGGGGCCTTCAGACCGACAGCT GCTGCTCTTCTACTTGGAGCAGTGCGAGGCCAACCTGACCACACTGACAGATGCAGTGGACGCCTTCTTC ACTGCGGTGGCCACCAACCAACCACCCAAGATCTTTGTGGCACACAGCAAGTTTGTCATTCTCAGTGCCC ACAAGCTTGTGTTCATTGGGGACACACTGTCACGGCAGGCAAAGGCAGCTGATGTCCGAAGCCAAGTGAC CCACTACAGCAATCTGCTGTGTGACCTCCTGCGTGGCATTGTGGCCACCACCAAGGCTGCTGCCCTGCAG TACCCATCCCCTTCCGCTGCCCAGGACATGGTGGACAGGGTCAAGGAGCTAGGCCACAGCACTCAGCAGT TCCGCCGCGTCCTGGGCCAGCTAGCTGCTGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198839
Insert Size	2625 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198839.1, NP_001185768.1
RefSeq Size	3146 bp
RefSeq ORF	2625 bp
Locus ID	12927
UniProt ID	Q61140
Gene Summary	Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration (By similarity). Has been shown to be essential in cardiovascular development during embryogenesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shorter transcript and encodes isoform A. Isoforms A and B are the same length but differ by 4 N-terminal amino acids.

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