Cd22 (NM_009845) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A530093D23; Lyb-8; Lyb8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229078 representing NM_009845 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGTCCATTACCTGTGGCTCCTCTTGATCCTAGGACACGTGGCTTCGGCTCGGTACAGCTCAGCAA ATGATTGGACCGTTGACCATCCCCAAACCCTCTTTGCCTGGGAGGGAGCCTGCATCAGGATTCCTTGCAA GTACAAAACTCCACTACCCAAGGCACGTCTGGACAACATCCTCCTTTTTCAGAACTATGAGTTTGACAAG GCCACCAAGAAATTCACAGGAACTGTCCTGTACAACGCCACAAAGACTGAGAAGGACCCAGAGTCTGAGC TGTACCTTTCTAAGCAAGGGAGAGTAACATTTCTGGGGAACAGAATAGACAATTGTACCCTGAAAATCCA CCCGATACGTGCCAATGACAGTGGGAATCTGGGGTTGAGGATGACCGCAGGGACTGAACGATGGATGGAG CCCATTCACCTCAATGTCTCGGAGAAACCGTTCCAACCTTACATCCAGATGCCATCAGAAATCCGGGAAT CCCAAAGTGTCACTCTGACCTGTGGACTGAATTTCTCCTGCTTTGGGTATGACATCCTCTTGAAGTGGTT CCTAGAAGATTCTGAGATCACCTCCATCACCTCTTCTGTCACCTCCATCACCTCTTCTGTCACCTCCTCC ATTAAGAATGTCTATACAGAGAGCAAGCTCACCTTCCAACCAAAGTGGACGGACCACGGAAAGAGTGTGA AGTGCCAAGTCCAGCACTCCTCCAAAGTGCTCTCAGAGCGCACTGTGCGTCTGGATGTTAAGTATACCCC GAAGCTGGAGATCAAGGTCAATCCCACAGAGGTGGAAAAGAACAATTCTGTGACCATGACATGCCGGGTT AACAGCAGCAACCCGAAACTCAGGACCGTGGCGGTGTCTTGGTTCAAGGATGGGCGCCCCCTAGAGGATC AGGAACTGGAACAGGAACAACAGATGTCCAAGCTAATTCTGCATTCAGTGACCAAGGACATGAGAGGGAA ATACCGGTGCCAGGCTTCCAACGACATAGGCCCAGGAGAGTCGGAAGAAGTGGAACTCACGGTGCACTAT GCTCCAGAACCCTCCAGGGTTCACATCTACCCTTCCCCCGCTGAAGAGGGACAGTCAGTAGAGCTGATTT GTGAGTCACTGGCCAGTCCAAGTGCAACAAACTACACCTGGTATCACAACAGGAAACCTATACCTGGAGA CACCCAAGAGAAGCTCCGCATCCCTAAAGTCTCCCCGTGGCATGCTGGGAATTACTCTTGCTTGGCAGAG AACCGTCTGGGTCATGGAAAGATAGACCAGGAAGCTAAGCTGGATGTCCATTATGCTCCCAAGGCGGTGA CCACAGTGATTCAGAGCTTCACACCAATCCTGGAAGGAGACAGTGTGACCCTGGTCTGTAGGTACAACTC CAGCAATCCAGACGTCACCTCCTACAGATGGAACCCTCAAGGTTCTGGGAGTGTGCTCAAACCCGGAGTG CTGAGGATTCAGAAAGTGACATGGGATTCCATGCCTGTCAGCTGTGCTGCCTGCAACCACAAGTGTTCGT GGGCCCTCCCTGTCATCCTGAATGTCCACTACGCCCCCAGAGACGTGAAGGTACTGAAGGTAAGCCCCGC ATCAGAGATCCGCGCTGGGCAGCGTGTCCTCCTCCAATGCGACTTCGCAGAGAGCAACCCGGCAGAGGTC CGCTTCTTCTGGAAGAAGAATGGGAGTCTCGTGCAGGAAGGGAGGTACCTGAGCTTCGGCTCCGTCTCCC CAGAAGATTCTGGAAATTATAACTGCATGGTCAACAACTCCATCGGAGAGACCTTGTCACAGGCCTGGAA CCTCCAAGTGCTGTATGCTCCTCGGAGGCTGCGTGTGTCCATCAGCCCTGGGGACCATGTGATGGAGGGG AAGAAGGCCACCTTGTCCTGTGAGAGTGATGCCAATCCGCCCATCTCACAGTACACCTGGTTTGACTCCA GTGGCCAAGACCTCCACTCCTCAGGCCAGAAACTGAGACTGGAACCCCTGGAGGTCCAACACACGGGTTC CTACCGCTGCAAAGGGACCAATGGGATAGGCACAGGAGAGTCACCACCCAGCACCCTCACTGTCTACTAC AGTCCAGAGACCATCGGCAAGCGGGTCGCCTTGGGACTAGGCTTCTGCCTGACTATCTGCATCCTGGCCA TCTGGGGGATGAAAATCCAGAAAAAATGGAAGCAAAACCGCAGCCAGCAGGGGCTTCAGGAAAATTCCAG TGGCCAGAGCTTTTTTGTGAGGAACAAAAAGGCTAGGAGGACCCCTCTCTCAGAAGGCCCCCAATCCCAG GGATGCTACAATCCGGCAATGGATGACACCGTTAGTTATGCCATCTTGCGCTTTCCAGAGAGTGACACGC ACAACACTGGAGATGCAGGGACCCCAGCAACACAGGCTCCTCCTCCAAACAACAGCGACTCGGTCACTTA CTCGGTGATACAGAAGCGGCCTATGGGGGATTATGAGAATGTGAATCCGAGCTGCCCGGAGGATGAGAGC ATCCATTACTCAGAGCTGGTTCAGTTTGGGGCTGGTAAGCGGCCCCAGGCAAAGGAAGACGTAGACTATG TGACCCTCAAGCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009845
Insert Size	2607 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009845.3, NP_033975.3
RefSeq Size	3972 bp
RefSeq ORF	2607 bp
Locus ID	12483
UniProt ID	P35329
Gene Summary	Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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