Hk3 (NM_001206392) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	HK-III; HK III
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229075 representing NM_001206392 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGGCACTGAAGGGACAGGACAGTCCCGCTCCTTCTGTCCGGATGCTGCCCACATACGTGAGGT CCACACCACACGGCACCGAGCAAGGGGATTTCCTGGTGCTGGAGCTGGGGGCCACAGGAGCATCACTACG TGTGTTGTGGGTAACACTGACAGGCACCAAAGAATGCCGGGTGGAGCCCAGGAGCCGGGAGTTTGTGATT CCTCAAGAGGTGATCCTAGGTGCTGGCCAGCAGCTCTTTGACTTTGCTGCCCGATGCCTCTCTGAATTCC TGGATGCATACCCCGTGGAGAATCAGGGTCTGAAGCTTGGGTTCAATTTCTCTTTTCCTTGTCACCAGAC AGGCTTGGACAGGAGCACCCTCATTTCCTGGACAAAAGGTTTTAGGTGCAGCGGCGTGGAAGGCCAGGAT GTGGTCCAGTTGCTAAGAGATGCCATTCAGAGGCAGGGGACCTACAGGATTGATGTGGTAGCCATGGTGA ATGACACGGTGGGCACCATGATGGGCTGTGAGCTGGGCACCAGGCCGTGTGAAGTCGGGCTGATTGTAGA CACCGGTACCAACGCGTGCTACATGGAGGAGGCGCGGCACGTGGCCGCTCTGGATGAGGACCGCGGCCGC ACCTGTGTCAGCATCGAGTGGGGCTCCTTCTATGACGAAGATGCTCTAGGGCCAGTACTGACCACCTTCG ACTCTGCCCTGGACCGCGAGTCCCTGACTCCTGGTGCTCAAAGGTTTGAGAAGATGATTGGAGGCCTTTA CCTGGGTGAGCTGGTGAGGCTGGTTCTGGTCCACTTGACCCAGCACGGGGTCCTCTTTGATGGCTGCGCC TCTCCTGCGTTGCTGAGTCAAGGCTGTATCCTCCTGGACCACGTGGCTGAGATGGAGGACACCGCCACTG GGACAGCCCGCGTCCACACCATCCTGCAGGACTTGGGTCTGAGCCCTCGGGCCTCAGATGCTGAACTGGT GCAATATGTGTGCGTGGCTGTGTGCACACGGGCTGCCCAGCTCTGTGCGGCTGCCCTGGCTGCAGTCCTC TCCCGCCTCCAGCACAGCAGGGAGCAGCAGACATTGCAAGTGGCTGTGGCCACTGGAGGGCGAGTGTTTG AACGACACCCCAGGTTCCTCCGAATCCTAAAGGAGACGGTAACACTCCTGGCCCCAAACTGTGATGTCTC CTTCATCCCCTCTGTGGATGGTGGTGGCCGGGGTGTGGCAATGGTGACAGCTGTGGCTGCCCGCCTGGCT GCTCACAGGCGCATCCTGGAAGAGACCCTGGCACCATTTCAGCTGACCTTGGAGCAGATGACAGTGGTGC AGGCACAAATGCGGGAAGCCATGATCAGGGGGCTTCAAGGAGAGGCCTCCTCCCTCCGCATGCTGCCCAC TTACGTCCGAGCGACGCCCGACGGCAGCGAGCGAGGTGATTTCCTGGCCTTGGACCTAGGGGGCACCAAC TTCCGGGTCCTGTTGGTACGAGTGGCGGAGGGCAGTGTGCAGATCATCAACCAGGTCTACTCCATTCCTG AGTGTAGAGCCCAGGGCTCTGGACAGAAGCTCTTTGACCATATTGTGGACTGCATCGTGGACTTCCAGAA GAGGCAGGGCCTTAGTGGACAGAGCCTACCCCTGGGTTTCACCTTCTCTTTCCCATGCAAGCAGCTTGGC CTGGACCAGGGCATCCTCCTGAATTGGACTAAGGGGTTCAATGCATCAGGCTGTGAGGGCCAGGATGTTG TGTATCTATTACGGGAAGCCATTAGGCGCAGACAGGCAGTGGAGCTGAATGTGGTTGCCATTGTCAATGA CACGGTGGGGACCATGATGTCCTGTGGCTATGATGATCCCCGTTGTGAGATGGGCCTCATTGTCGGAACC GGCACCAACGCCTGTTACATGGAAGAGCTCCGCAATGTGGCAAGTGTGCCTGGGGACTCGGGCCTCATGT GCATCAACATGGAGTGGGGTGCCTTTGGGGATGATGGCTCACTGGGCACACTCAGCACCCGCTTTGACAC CAGTGTGGACCAGGCATCCATCAATCCAGGCAAACAGAGGTTTGAGAAAATGATCAGCGGTATGTACTTG GGGGAGATCGTCCGCCATATCCTCCTGCACTTAACCAATCTCGGAGTTCTCTTCCGAGGCCAGAAGACTC AATGCCTTCAGGCCAGGGACATCTTCAAGACTAAGTTCCTCTCTGAGATCGAAAGTGATAGCCTGGCCCT GCGTCAGGTCCGAGCCATCCTGGAAGATCTGGGGCTGACTCTGACATCTGATGACGCCTTGATGGTCCTG GAAGTGTGCCAGGCTGTGTCTCGCAGGGCTGCCCAACTCTGTGGGGCAGGTGTGGCTGCCGTGGTGGAGA AGATTCGGGAGAACCGGGGCCTGCAGGAGCTGACAGTGTCTGTGGGAGTAGATGGGACACTCTACAAGCT ACATCCTCACTTCTCCAAGCTGGTGTCGGCAACAGTCCGGAAGCTAGCCCCTCAGTGCACCGTCACCTTT TTGCAGTCGGAGGATGGGTCTGGCAAAGGCGCAGCTTTGGTTACTGCTGTTGCTTGTCGCCTTACCCAGA TGGCCCACGTCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001206392
Insert Size	2604 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206392.1, NP_001193321.1
RefSeq Size	3099 bp
RefSeq ORF	2604 bp
Locus ID	212032
Gene Summary	Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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