Rapgef4 (NM_001204166) Mouse Untagged Clone

CAT#: MC229074

Rapgef4 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), transcript variant 3



  "NM_001204166" in other vectors (1)

CNY 8,360.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1300003D15Rik; 5730402K07Rik; 6330581N18Rik; Epac-2; EPAC 2; Epac2; mKIAA4040
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229074 representing NM_001204166
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCTACGAGAAATACCGACAGTATATGGCCGGACTTCTGGCTCCTCCCTATGGTGTTATGGAAACGG
GCTCTAACAATGACAGGATTCCTGACAAGGAGAATACACCTCTCATTGAACCCCACGTTCCTCTCCGTCC
TGCTCACACCATTACCAAGGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAATTGCCATT
CTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCACCTAAAGACATACAGACAATGCTGTGTTG
GGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTGTTCACTCGCGGACTCAAGCTGTTGGCAT
GTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATGTGGACCAGGAGCGCCATTTCCAAGACAAATAT
TTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCTTTGCCTACTGAGGAAGAGAAGAAGGAGT
GTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGATGGGCCCTGACGCCCACATGAGAATGAT
CCTGCGAAAACCACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTACGACGAGCTCCTTCATATT
AAAGCCTTATCCCATCTCTCTACCACAGTGAAACGGGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACG
CCAAAGGAGGAACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATCATTCTGAAAGGATC
CGTGAATGTAGTCATTTATGGCAAGGGTGTGGTCTGCACCCTGCACGAAGGAGATGACTTTGGCAAGTTA
GCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTTCGGGAAGATAATTGTCACTTCCTAAGAG
TCGACAAGGAAGACTTCAATCGGATTCTGAGGGACGTTGAGGCGAATACAGTCAGACTTAAAGAACATGA
CCAAGATGTCTTGGTACTGGAGAAGGTCCCAGCAGGGAACAGAGCTGCTAATCAAGGAAACTCACAGCCT
CAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAGATTTTAGAGCATTTTCTAGAAACAATACGCC
TTGAGCCATCGTTGAATGAAGCAACAGATTCGGTTTTAAATGACTTTGTTATGATGCACTGTGTTTTTAT
GCCAAATACCCAGCTTTGCCCTGCCCTTGTGGCCCATTACCACGCACAGCCTTCTCAAGGTACCGAGCAG
GAGAGAATGGATTATGCCCTCAACAACAAGAGGCGGGTCATCCGCTTGGTCCTGCAGTGGGCGGCCATGT
ATGGCGATCTCCTCCAAGAAGATGATGTGGCCATGGCCTTCCTGGAGGAGTTCTATGTGTCTGTATCAGA
TGACGCACGGATGATGGCTGCCTTCAAGGAGCAGCTGCCAGAGCTGGAGAAGATTGTCAAGCAAATCTCA
GAAGACGCAAAAGCTCCACAGAAGAAGCACAAGGTGCTTTTGCAACAGTTCAACACAGGTGACGAGAGGG
CCCAGAAGCGTCAGCCTATTCGTGGCTCTGATGAGGTTTTGTTCAAGGTCTACTGCATCGACCACACCTA
TACTACCATTCGTGTGCCGGTAGCTGCCTCGGTGAAGGAAGTCATCAGTGCAGTAGCTGACAAACTGGGC
TCAGGGGAAGGCCTGATCATCGTCAAGATGAACTCTGGAGGAGAAAAGGTGGTGCTGAAATCTAATGATG
TTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTGCCTGCCCGAGAGAGCAATTCGACTCACT
GACTCCCTTGCCGGAACAGGAAGGCCCGACCACTGGGACAGTGGGAACATTTGAGCTGATGAGCTCGAAA
GACCTGGCGTACCAGATGACAACCTACGATTGGGAACTCTTCAACTGTGTGCATGAGCTGGAGCTAATCT
ACCACACATTTGGAAGGCATAATTTTAAAAAGACCACGGCAAACTTGGATTTGTTCCTGAGGAGGTTTAA
TGAAATTCAGTTTTGGGTTGTCACTGAGGTCTGCCTTTGTTCCCAGCTCAGCAAACGTGTTCAGCTTTTG
AAAAAATTTATCAAGATAGCGGCTCACTGCAAGGAGTACAAAAATCTAAATTCCTTTTTCGCCATCGTCA
TGGGACTCAGCAACGTGGCCGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCGAGCAAGTTTAAGAA
GTTCTATGCGGAGTTTGAGAGCTTGATGGATCCTTCCAGAAACCACAGGGCATACAGGCTGACAGCAGCC
AAGCTGGAGCCCCCTCTCATCCCTTTCATGCCCTTGCTTATTAAAGATATGACATTTACTCATGAGGGGA
ACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAACACTGCCAGAACAGT
ACGGTACTACAGGAGCCAGCCCTTCAATCCGGATGCCGCTCAAGCTAATAAGAACCATCAGGATGTCCGG
AGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCACACAGATTAGAGC
CTCGAAGGCCATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001204166
Insert Size 2604 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001204166.1, NP_001191095.1
RefSeq Size 4112 bp
RefSeq ORF 2604 bp
Locus ID 56508
UniProt ID Q9EQZ6
Gene Summary Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon in an alternate exon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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