Rapgef4 (NM_001204166) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1300003D15Rik; 5730402K07Rik; 6330581N18Rik; Epac-2; EPAC 2; Epac2; mKIAA4040
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229074 representing NM_001204166 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTACGAGAAATACCGACAGTATATGGCCGGACTTCTGGCTCCTCCCTATGGTGTTATGGAAACGG GCTCTAACAATGACAGGATTCCTGACAAGGAGAATACACCTCTCATTGAACCCCACGTTCCTCTCCGTCC TGCTCACACCATTACCAAGGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAATTGCCATT CTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCACCTAAAGACATACAGACAATGCTGTGTTG GGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTGTTCACTCGCGGACTCAAGCTGTTGGCAT GTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATGTGGACCAGGAGCGCCATTTCCAAGACAAATAT TTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCTTTGCCTACTGAGGAAGAGAAGAAGGAGT GTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGATGGGCCCTGACGCCCACATGAGAATGAT CCTGCGAAAACCACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTACGACGAGCTCCTTCATATT AAAGCCTTATCCCATCTCTCTACCACAGTGAAACGGGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACG CCAAAGGAGGAACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATCATTCTGAAAGGATC CGTGAATGTAGTCATTTATGGCAAGGGTGTGGTCTGCACCCTGCACGAAGGAGATGACTTTGGCAAGTTA GCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTTCGGGAAGATAATTGTCACTTCCTAAGAG TCGACAAGGAAGACTTCAATCGGATTCTGAGGGACGTTGAGGCGAATACAGTCAGACTTAAAGAACATGA CCAAGATGTCTTGGTACTGGAGAAGGTCCCAGCAGGGAACAGAGCTGCTAATCAAGGAAACTCACAGCCT CAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAGATTTTAGAGCATTTTCTAGAAACAATACGCC TTGAGCCATCGTTGAATGAAGCAACAGATTCGGTTTTAAATGACTTTGTTATGATGCACTGTGTTTTTAT GCCAAATACCCAGCTTTGCCCTGCCCTTGTGGCCCATTACCACGCACAGCCTTCTCAAGGTACCGAGCAG GAGAGAATGGATTATGCCCTCAACAACAAGAGGCGGGTCATCCGCTTGGTCCTGCAGTGGGCGGCCATGT ATGGCGATCTCCTCCAAGAAGATGATGTGGCCATGGCCTTCCTGGAGGAGTTCTATGTGTCTGTATCAGA TGACGCACGGATGATGGCTGCCTTCAAGGAGCAGCTGCCAGAGCTGGAGAAGATTGTCAAGCAAATCTCA GAAGACGCAAAAGCTCCACAGAAGAAGCACAAGGTGCTTTTGCAACAGTTCAACACAGGTGACGAGAGGG CCCAGAAGCGTCAGCCTATTCGTGGCTCTGATGAGGTTTTGTTCAAGGTCTACTGCATCGACCACACCTA TACTACCATTCGTGTGCCGGTAGCTGCCTCGGTGAAGGAAGTCATCAGTGCAGTAGCTGACAAACTGGGC TCAGGGGAAGGCCTGATCATCGTCAAGATGAACTCTGGAGGAGAAAAGGTGGTGCTGAAATCTAATGATG TTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTGCCTGCCCGAGAGAGCAATTCGACTCACT GACTCCCTTGCCGGAACAGGAAGGCCCGACCACTGGGACAGTGGGAACATTTGAGCTGATGAGCTCGAAA GACCTGGCGTACCAGATGACAACCTACGATTGGGAACTCTTCAACTGTGTGCATGAGCTGGAGCTAATCT ACCACACATTTGGAAGGCATAATTTTAAAAAGACCACGGCAAACTTGGATTTGTTCCTGAGGAGGTTTAA TGAAATTCAGTTTTGGGTTGTCACTGAGGTCTGCCTTTGTTCCCAGCTCAGCAAACGTGTTCAGCTTTTG AAAAAATTTATCAAGATAGCGGCTCACTGCAAGGAGTACAAAAATCTAAATTCCTTTTTCGCCATCGTCA TGGGACTCAGCAACGTGGCCGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCGAGCAAGTTTAAGAA GTTCTATGCGGAGTTTGAGAGCTTGATGGATCCTTCCAGAAACCACAGGGCATACAGGCTGACAGCAGCC AAGCTGGAGCCCCCTCTCATCCCTTTCATGCCCTTGCTTATTAAAGATATGACATTTACTCATGAGGGGA ACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAACACTGCCAGAACAGT ACGGTACTACAGGAGCCAGCCCTTCAATCCGGATGCCGCTCAAGCTAATAAGAACCATCAGGATGTCCGG AGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCACACAGATTAGAGC CTCGAAGGCCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204166
Insert Size	2604 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204166.1, NP_001191095.1
RefSeq Size	4112 bp
RefSeq ORF	2604 bp
Locus ID	56508
UniProt ID	Q9EQZ6
Gene Summary	Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon in an alternate exon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1.

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