Trpc7 (NM_001302372) Mouse Untagged Clone

CAT#: MC229061

Trpc7 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7), transcript variant 2



  "NM_001302372" in other vectors (1)

CNY 8,360.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms TR; TRP7; Trrp8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229061 representing NM_001302372
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGGGAGCAACACCTTCAAAAACATGCAGCGCCGGCACACCACCTTGAGGGAGAAGGGGCGCCGCC
AGGCCATCCGGGGTCCTGCCTACATGTTCAACGAGAAGGGCACGAGCCTGACCCCTGAGGAGGAGCGCTT
CCTGGACTCGGCTGAGTATGGCAACATACCAGTGGTCAGAAAAATGCTGGAGGAATCCAAGACCCTCAAT
TTCAACTGCGTGGACTACATGGGGCAGAACGCGCTGCAGCTGGCCGTGGGCAACGAGCACTTGGAGGTCA
CTGAACTGTTGCTGAAGAAAGAGAACTTGGCGCGGGTGGGCGACGCGCTGCTGCTGGCCATCAGCAAGGG
CTATGTGCGCATTGTGGAGGCCATCCTCAGCCATCCGGCCTTCGCGCAGGGCCAGCGCCTGACGCTCAGC
CCGCTGGAGCAGGAGCTGCGGGATGATGACTTCTATGCCTACGACGAGGATGGCACGCGCTTCTCCCACG
ACATCACACCCATCATCCTGGCGGCCCACTGCCAGGAGTATGAGATTGTGCATATCTTGCTGCTCAAGGG
TGCGCGCATTGAGCGGCCCCATGACTACTTCTGCAAGTGCAATGAGTGCACGGAGAAGCAGCGCAAGGAC
TCCTTCAGTCACTCGCGCTCCCGGATGAATGCCTACAAAGGACTGGCCAGTGCCGCCTACCTGTCCCTAT
CCAGTGAAGACCCTGTCCTCACTGCGCTGGAGCTAAGCAACGAGTTAGCCCGACTGGCCAACATTGAGAC
TGAATTTAAGAATGATTACAGGAAGTTATCTATGCAATGCAAGGATTTTGTGGTGGGCGTGCTGGACCTG
TGCCGAGACACAGAAGAGGTGGAAGCAATTTTAAATGGCGATGTGAACTTGCAAGTCTGGTCCGACCACC
ACCGTCCAAGTCTGAGCCGAATCAAACTCGCCATTAAGTACGAAGTCAAGAAGTTCGTTGCTCACCCTAA
CTGTCAGCAGCAATTGCTTACCATGTGGTATGAAAATCTCTCAGGCTTACGGCAACAGTCTATCGCTGTG
AAATTCCTGGCTGTCTTTGGAGTCTCCATAGGCCTCCCTTTTCTCGCCATAGCCTATTGGATTGCTCCGT
GCAGCAAGCTAGGACAAACCCTGAGGAGCCCTTTCATGAAGTTTGTGGCCCATGCAGTGTCTTTTACCAT
CTTCTTGGGACTGCTAGTTGTGAACGCCTCCGACCGGTTTGAGGGCGTTAAGACTCTGCCCAATGAGACC
TTCACAGACTACCCCAAACAGATCTTCAGAGTGAAAACCACACAGTTCTCCTGGACGGAGATGCTCATCA
TGAAGTGGGTCTTAGGAATGATCTGGTCCGAATGCAAGGAGATCTGGGAGGAGGGGCCGCGGGAGTACGT
GCTACACTTGTGGAACCTTCTGGATTTTGGCATGCTGTCCATCTTCGTGGCCTCCTTCACTGCGAGGTTC
ATGGCTTTCCTCAAGGCCAGCGAGGCCCAGCTGTACGTGGACCAGTACGTGCAGGATGTAACGCTGCACA
ACGTCTCACTTCCACCGGAAGTGGCCTACTTCACCTACGCCAGGGATAAGTGGTGGCCTTCAGACCCCCA
GATCATCTCGGAAGGGCTGTACGCCATTGCGGTTGTCCTCAGCTTCTCCCGGATCGCCTACATCCTGCCA
GCCAATGAGAGCTTCGGACCTTTGCAGATCTCCCTGGGAAGAACTGTGAAAGACATCTTCAAGTTCATGG
TCATTTTTATCATGGTATTTGTGGCCTTTATGATCGGAATGTTCAACCTGTACTCCTACTACCGAGGTGC
AAAGTACAACCCAGCGTTTACCACAGTTGAAGAAAGCTTTAAAACCTTGTTTTGGTCCATATTTGGCTTG
TCTGAGGTCATCTCGGTGGTGCTGAAATACGACCACAAGTTCATCGAGAATATTGGCTACGTGCTGTATG
GGGTTTATAATGTCACCATGGTGGTTGTACTACTCAACATGCTGATAGCCATGATCAACAACTCCTATCA
GGAAATCGAGGAAGACGCGGACGTGGAGTGGAAATTTGCTCGAGCGAAGCTCTGGCTTTCTTACTTTGAT
GAAGGAAGAACTCTCCCTGCTCCCTTTAACCTGGTGCCGAGCCCTAAATCCTTTTATTATCTCATAATGA
GAATCAAGATGTGCCTCATAGAGCTCTGCCAATCTAAGGCCAAACGCTGTGAAAACGACCTGGAAATGGG
CATGCTGAACTCCAAGTTCAGGACTCGCTACCAGGCTGGCATGAGGAATTCTGAAAACCTGACAGCCAAT
AGCACCTTCAGCAAGCCCACCAGATACCAGAAGATCATGAAGCGGCTCATAAAGAGATACGTGCTGAAGG
CCCAGGTGGACAGAGAGAACGATGAAGTCAATGAAGGTGAACTCAAGGAGATCAAACAAGACATCTCAAG
CCTTCGCTATGAGCTCCTGGAGGAGAAGTCTCAGGCTACAGGAGAGCTGGCAGACCTGATCCAGCAGCTC
AGCGAGAAGTTTGGGAAGAATCTGAACAAAGACCACCTGCGGGTGAACCAGGGCAAGGACATTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001302372
Insert Size 2586 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001302372.1, NP_001289301.1
RefSeq Size 3547 bp
RefSeq ORF 2586 bp
Locus ID 26946
Gene Summary The protein encoded by this gene is a member of the transient receptor potential channel family of proteins, which form six-transmembrane cation-permeable channels that are calcium permeant. Knock out mice are viable but display a reduction in the gamma wave activity that precedes seizure induction in response to a muscrarinic agonist, suggesting a functional role for this protein in initiation of seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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