Sidt2 (NM_001289668) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	B930096O19; BC023957; CGI-40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229050 representing NM_001289668 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCCTGGCGTCTGCCCTTGTGCGTGCTCTTGGTGGCCTCCGTCGAGAGCCACCTGGGGGCCCTGG GGCCCAAGAACGTCTCGCAGAAAGACGCGGAGTTTGAGCGCACCTACGCGGACGACGTCAACAGCGAGCT GGTCAACATCTACACCTTCAACCACACCGTGACCCGCAACCGGACCGAGGGTGTGCGAGTGTCTGTGAAT GTCCTGAACAAGCAGAAAGGGGCGCCTTTGCTGTTTGTGGTCCGCCAGAAGGAGGCTGTTGTGTCCTTCC AGGTGCCCCTAATCCTTCGAGGACTATATCAGCGGAAGTACCTCTACCAAAAAGTGGAACGAACTCTGTG TCAGCCCCCCACCAAGAATGAGTCTGAGATCCAGTTTTTCTATGTGGACGTGTCTACCCTGTCACCCGTC AATACCACTTACCAGCTCCGAGTCAACCGTGTGGACAATTTTGTGCTCAGGACTGGAGAGCTGTTTACTT TTAATACCACTGCAGCCCAGCCCCAGTACTTCAAATACGAGTTTCCTGATGGTGTGGACTCGGTAATTGT CAAGGTGACCTCCAAGAAGGCCTTCCCCTGCTCAGTCATCTCCATCCAAGATGTCCTGTGCCCTGTCTAT GATCTGGACAACAATGTAGCCTTCATTGGCATGTACCAGACGATGACTAAGAAGGCAGCCATCACTGTGC AGCGGAAAGACTTCCCCAGCAACAGCTTCTATGTGGTGGTGGTAGTGAAGACTGAGGACCAGGCCTGCGG AGGGTCCTTGCCCTTCTACCCTTTTGTGGAAGATGAGCCAGTGGATCAAGGGCACCGTCAGAAAACACTG TCAGTGCTGGTCTCTCAGGCTGTCACATCTGAGGCCTATGTTGGTGGGATGCTCTTTTGCCTGGGCATAT TCTTGTCCTTCTACCTGCTGACTGTGCTGCTGGCCTGTTGGGAGAACTGGAGGCAAAGGAAGAAGACCTT GCTGTTGGCCATAGACCGAGCCTGCCCAGAAAGTGGTCACGCCCGGGTCTTGGCTGATTCATTTCCTGGC AGTGCCCCTTACGAGGGTTACAACTATGGCTCCTTTGAAAATGGTTCCGGATCCACTGACGGGTTGGTTG AAAGCGCAGGTTCAGGGGACCTCTCCTACAGTTACCAGGGGCACGACCAGTTCAAGCGGCGCCTTCCCTC TGGCCAGATGCGGCAGCTGTGCATTGCCATGGACCGCTCCTTTGACGCAGTGGGTCCTCGGCCTCGACTG GACTCCATGAGCTCCGTGGAAGAGGATGACTACGACACACTGACTGACATCGACTCAGACAAAAACGTCA TTCGAACCAAGCAATACCTCTGTGTGGCTGATCTGGCACGAAAGGACAAACGTGTTTTGCGGAAAAAGTA CCAGATTTACTTCTGGAACATAGCCACCATTGCGGTCTTCTACGCACTTCCTGTGGTGCAGCTGGTGATC ACCTACCAGACGGTGGTGAATGTCACAGGGAACCAGGACATCTGCTACTACAACTTCCTCTGTGCCCACC CGCTGGGCAACCTCAGCGCCTTCAACAACATCCTCAGCAACTTGGGGTACATCCTGCTGGGGCTGCTCTT CCTGCTCATCATCCTGCAGCGAGAGATCAATCATAACCGGGCCCTGCTGCGGAATGACCTCTATGCTCTG GAGTGTGGGATCCCCAAACACTTTGGTCTGTTTTACGCCATGGGCACAGCACTGATGATGGAGGGGCTAC TTAGTGCCTGTTACCACGTCTGCCCCAACTACACCAACTTCCAGTTTGATACCTCCTTCATGTACATGAT TGCTGGCCTCTGCATGCTGAAGCTCTACCAGAAGCGGCACCCAGATATCAACGCCAGTGCCTACAGTGCA TATGCCTGCTTGGCCATCGTCATCTTCTTCTCCGTTCTGGGCGTGGTGTTTGGCAAAGGGAACACGGCCT TCTGGATTGTCTTCTCCGTCATTCACATCATCTCCACCCTGCTCCTCAGCACTCAGCTCTATTACATGGG CCGCTGGAAGCTGGACTCCGGGATCTTCCGCCGCATCCTCCATGTGCTCTACACAGACTGCATCCGGCAG TGCAGCGGGCCCCTTTACACGGACCGCATGGTGCTTCTGGTCATGGGCAACATTATCAACTGGTCGCTGG CTGCATACGGACTCATCATGCGCCCCAATGACTTTGCTTCCTACTTGCTGGCAATTGGCATCTGCAACCT GCTGCTTTATTTCGCCTTCTACATCATCATGAAGCTCCGGAGCGGCGAGAGGATCAAGCTCATCCCTCTG CTCTGCATCGTCTGCACCTCCGTGGTCTGGGGCTTCGCGCTCTTCTTCTTCTTCCAGGGACTGAGCACGT GGCAGAAAACCCCCGCAGAGTCCAGGGAGCACAACCGCGACTGCATCCTCCTCGACTTCTTTGATGACCA CGATATCTGGCACTTCCTGTCCTCCATTGCCATGTTTGGGTCCTTCCTGGTTTTGCTGACGTTGGATGAC GACTTGGACACAGTACAGCGGGACAAGATCTATGTCTTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289668
Insert Size	2562 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289668.1, NP_001276597.1
RefSeq Size	4302 bp
RefSeq ORF	2562 bp
Locus ID	214597
UniProt ID	Q8CIF6
Gene Summary	Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA and DNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251, PubMed:27846365, PubMed:28724756). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (PubMed:28277980). In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA (PubMed:27846365). Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (PubMed:26067272).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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