Amot (NM_001290274) Mouse Untagged Clone

CAT#: MC229049

Amot (untagged) - Mouse angiomotin (Amot), transcript variant 2



  "NM_001290274" in other vectors (1)

CNY 8,270.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CAG-2; D0Kist1; Sii6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229049 representing NM_001290274
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAAGTTCTGACGATCAGCCAAGTGGAGGAACGACGGTACTGCAACGGCTGCTTCAAGAGCAGCTTC
GCTATGGCAATCCCAGTGAGAATCGCAGTCTGCTTGCCATACACCAGCAAGCCACAGGGAATAGCTCTCC
ATTTTCCACGGGCAGTGGGAACCAAGGCCCTCAGAATGATGTATTGAGTTCCCAGGATCACCACCAGCAG
CAGCTTGTGGCACACCCTGCTCGCCAAGAGCCCCAGGGTCAGGAAATCCAGTCAGAAAACGGCGTCATGG
AGAAGCAGCTGTCCCCTCGAATGCAGAATAACGAAGAACTCCCAACCTATGAAGAGGCCAAGGTCCAGTC
CCAGTACTTCAGGGGCCAGCAGCATGCTAGTGTTGGGGCTGCTTTCTATGTCACAGGAGTCACCAACCAG
AAGATGAGAACCGAGGGACGCCCGTCAGTCCAGCGCCTCACGCCCGGGAAGATGCACCAAGATGAAGGCC
TTCGAGATCTTAAGCAAGGGCATGTCCGATCCTTGAGTGAAAGACTGATGCAGATGTCATTGGCTACCAG
TGGAGTTAAAGCCCACCCACCTGTTACCAGTGCTCCTCTCTCCCCACCACAACCCAATGATCTCTACAAG
AATGCCACCAGTTCCAGTGAATTCTACAAGGCCCAAGGGCCACCTCCAAGCCAACATAGTCTGAAGGGCA
TGGAACACCGAGGTCCCCCGCCAGAATACCCTTTCAAGGGCGTGCCATCCCAGTCTGTAGTGTGCAAGTC
CCAAGAGCCAGGGCACTTCTATAGTGAGCATCGCCTGAACCAGCCAGGGAGAACAGAGGGGCAACTGATG
AGGTATCAGCACCCCCCTGAGTATGGGGCAGCCAGGGCAACACAGGACATCTCCTCGCTGTCTTTGTCAG
CCAGGAACTCTCAACCTCATAGCCCTACTTCTTCCCTGACAGCTGGGGCTTCCAGCTTGCCCTTGCTGCA
GTCTCCGCCGTCCACTAGATTGCCTCCTGGTCAACACCTGGTCTCGAACCAAGGTGACCACTCTGCTCAC
CTGTCAAGGCATCAGCAGCATCTTCTTTCGAGTCAGAGTCATCAGGGGGACCACTACCGTCACGCCCAGG
CCAGCCTGACTTCAGCGCAGCAGCAGCCAGGAGAAGCCTACTCAGCTATGCCTCGGGCTCAGCAGTCTGC
TTCTTATCAGCCCATGCCAGCTGACCCTTTTGCTATGGTCTCTAGAGCTCAGCAGATGGTTGAGATCCTG
TCTGATGAGAATCGCAACTTGCGGCAGGAGTTGGATGGATGCTATGAGAAGGTGGCGAGACTGCAGAAGG
TGGAGACAGAAATCCAACGGGTCTCAGAGGCTTATGAGAACTTGGTGAAGTCATCTTCCAAAAGAGAGGC
TCTGGAGAAAGCCATGAGGAACAAGCTGGAGGGCGAGATTAGAAGGATGCATGACTTCAACAGAGATCTG
AGAGACCGTCTAGAGACTGCCAACAAGCAGCTGGCAGAGAAGGAGTACGAGGGGTCCGAGGACACCAGGA
AGACCATCTCGCAGCTCTTTGCCAAACATAAAGAAAACCAGCGGGAGAAGGAGAAGCTGGAAGCTGAGCT
GGCTACTGCCCGTTCTACCAATGAAGACCAAAGGCGACACATTGAAATCCGAGACCAGGCATTGAGCAAT
GCCCAGGCGAAGGTGGTGAAGCTGGAAGAAGAGCTGAAAAAGAAGCAAGTGTACGTAGATAAGGTGGAGA
AGATGCAGCAGGCCCTTGTGCAGCTCCAGGCCGCCTGTGAGAAGCGTGAGCAGCTGGAGCACCGGCTCCG
GACACGTTTGGAGAGGGAACTGGAATCCCTTAGAATCCAGCAGCGCCAGGGCAACTCCCAGCCCACTAAT
GCTTCAGAATACAATGCCGCTGCACTGATGGAGCTCCTCCGGGAGAAGGAAGAGAGAATTCTGGCCCTGG
AAGCAGATATGACCAAGTGGGAGCAAAAGTATTTGGAAGAGAATGTGATGAGACACTTTGCTCTGGATGC
TGCTGCAACTGTGGCTGCACAGAGAGACACAACAGTCATTAGCCACTCTCCTAACACCAGCTATGACACA
GCCTTAGAGGCCCGCATCCAGAAGGAAGAGGAAGAAATCCTGATGGCCAACAAGCGTTGCTTGGACATGG
AAGGAAGGATTAAGACCCTCCATGCCCAGATTATTGAAAAGGATGCCATGATCAAGGTGCTGCAGCAGCG
CTCCAGAAAGGAGCCGAGTAAAACAGAGCAACTCTCATCCATGCGGCCAGCAAAGTCTCTGATGTCCATT
TCCAATGCTGGATCAGGCCTCCTTGCACACTCCTCTACCTTGACTGGTGCGCCCATTATGGAGGAGAAGC
GTGATGACAAGAGCTGGAAGGGGAGCCTAGGTAAGGAAAAGGAAAGCAACAGAAGCAAAGGCACAGTGAC
AGACTTAGAAAGTGTCCTGACTCTGTTACACACAGCAAGGAAAAGGGACAATGGTCCAGGTTCTAGAGAG
GAGAATTTGGAGTCCCCCCTCTCAATGGAATTAGATTTATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001290274
Insert Size 2562 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001290274.1, NP_001277203.1
RefSeq Size 2937 bp
RefSeq ORF 2562 bp
Locus ID 27494
UniProt ID Q8VHG2
Gene Summary Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks several exons and it's 3'-terminal exon extends past a splice site that is used in variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...