Gripap1 (NM_001290455) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI854681; DXImx47e; GRASP-1; mKIAA1167; Sfc10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229022 representing NM_001290455 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAAGCTCTGTCTGAAGAGGAGTTTCAACGGATGCAGACTCAGCTCCTGGAGCTCCGGACAAATA ACTACCAGCTTTCGGATGAACTTCGGAAGAATGGTGTTGAACTTTCCAGTCTTCGACAGAAGGTCGCCTA CCTGGATAAGGAGTTCAGTAAAGCTCAAAAGGCACTAAGCAAGAGCAAGAAAGCTCAGGAAGTGGAGGTA CTGCTGAGTGAAAATGAGATGCTGCAGGCAAAGCTGCACAGCCAGGAGGAGGACTTCCGTTTGCAGAACA GTACACTTATGGCCGAGTTCAGCAAGCTTTGCAGCCAGCTGGAGCAGCTGGAGCTGGAAAACCGACAACT CAAGGAAGGCGTTCCTGGAGCAGCTGGAGCACATGTGGACGGGGAGCTGCTGAGGCTGCAGGCAGAGAAC ACAGCCTTGCAGAAGAACATGGCAGCACTGCAGGAACGCTATGGGAAAGAGGCTGTGAGGCCCTCTGCTG TTGGTGAGGGCCAAGGGGATCCCCCAGGAGACGTACTCCCCACTCCCCTGGCTCCCATGCCATTGGCAGA AGTGGAGCTGAAATGGGAAATGGAGCGAGAAGAAAAAAAATTGCTCTGGGAGCAGCTACAAGGCTTAGAG AGCTCAAAGCAAGCTGAAACCTCCAGACTACAGGAGGAACTTGCTAAGCTCTCTGAGAAATTGAAAAAGA AACAAGAAAGTTTTTGCCGTCTGCAGACAGAGAAGGAAACACTGTTTAATGACAGCAGAAACAAAATTGA AGAATTGCAACAGCGGAAGGAAGCTGATCTGAAAGCCCAGTTGGCTCGCACCCAAAAACTACAGCAGGAA CTTGAGGCTGCCAATCAGAGTTTGGCAGAGCTAAGAGATCAGCGGCAGGGGGAGCGTCTGGAGCATGCAG CAGCGTTGCGGGCCCTACAAGATCAGGTGTCCAGCCAGAGTGCAGATGCGCAGGAACAAGTGGAAGGGCT CTTGGCTGAGAACAGTGCCTTGAGGACTAGCTTGGCTGCTCTGGAGCAGATCCAGACAGCAAAGACACAA GAACTGAATATGCTTCGGGAACAGACCAGTGAATTGGCATCTGAGTTACAGCATCGTCAAGCTGAATATG AAGAGCTTATGGGACAGAAAGATGACCTCAACTCGCAGCTCCAGGAGTCGTTAAGGGCCAATAGCCGGCT GCTGGAACAACTTCAAGAAATAGGGCAGGAGAAGGAGCAGCTAACCCAGGATCTCCAGGAAGCTCGGAAG AGTGCCGAGAAAAGGAAGGTCATGCTGGATGAACTAGCCATGGAGACGCTGCAGGAGAAGTCCCAGCACA AAGAAGAACTGGGGGCCGTCCGGTTACGGCACGAGAAGGAGCTGCTGGGCGTGCGAGCCCGATACGAGCG TGAGCTTCGAGAACTGCACGAGGACAAGAAGCGGCAGGAGGAGGAGCTCCGGGGGCAGATTCGTGAGGAG AAGGCTCGAACAAGGGAATTAGAAAATCTTCAGCACACAGTGGAAGAACTCCAGGCTCAGGTACACTCCA TGGATGGAGCCAAGGGCTGGTTTGAACGGCGCTTGAAGGAAGCTGAGGAATCTTTGCAGCAGCAGCAACA GGAACAAGAGGAAACCCTCAAGCTGTGCCGGGAGGAGCACGCCGCTGAGCTGAAGGGCAAGGATGAAGAG CTTCAGAATGTTCGGGAACAGCTCCAGCAGGCCCAGGAGGAGCGAGATGGCCACGTGAAGACCATCAGCA ACCTGAAGCAGGAGGTAAAGGACACAGTAGACGGGCAGCGCATCCTGGAGAAGAAAGGCAGCGCTGTGCT CAAAGATCTTAAGCGGCAGCTGCACTTGGAGCGGAAACGGGCTGATAAGCTGCAGGAACGGCTGCAGGAG ATCCTTACCAACAGCAAGAGCCGCACAGGCCTCGAGGAGCTGGTTCTGTCTGAGATGAACTCACCAAGCA GGACCCAGACAGGGGACAGCAGTAGTGTCTCCTCCTTCAGCTACCGGGAGATCTTGAAGGAGAAGGAGAG TTCAGCCATCCCAGCCAGGTCTCTATCCAGCAGTCCTCAGGCCCAGCCCCCTCGGCCAGCAGAGCTGTCA GATGAGGAAGTGGCTGAGCTGTTTCAGAGGCTGGCCGAGACTCAGCAGGAAAAATGGATGCTAGAGGAGA AGGTGAAACACCTGGAGGTGAGCAGCGCCTCTATGGCGGAAGATCTCTGCCGGAAAAGCGCCATCATTGA GACCTACGTCATGGACAGCCGGATTGATGTGTCTGTGGCAGCAGGCCACACAGACCGAAGTGGGCTGGGC AGTGTCCTGAGAGACCTAGTGAAGCCAGGCGATGAGAACCTTCGAGAAATGAACAAGAAGCTGCAGAACA TGTTGGAGGAGCAACTCACCAAGAACATGCACTTGCACAAGGACATGGAAGTTCTATCCCAGGAAATTGT GCGGCTCAGCAAGGAATGTGTGGGGTCCCCTGACCCAGACCTAGAACCTGGAGAAGCCAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290455
Insert Size	2514 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290455.1, NP_001277384.1
RefSeq Size	3021 bp
RefSeq ORF	2514 bp
Locus ID	54645
Gene Summary	Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity).[UniProtKB/Swiss-Prot Function]

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