Atp6v0a1 (NM_001243050) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA959968; ATP6a1; Atp6n1; Atp6n1a; Atpv0a1; Vpp-1; Vpp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229014 representing NM_001243050 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGCTTTTCCGGAGTGAGGAGATGACGCTGGCCCAGCTATTTCTACAGTCTGAAGCTGCTTATT GCTGTGTCAGTGAATTAGGAGAGCTTGGGAAGGTCCAATTTCGGGATTTAAATCCAGATGTGAATGTTTT CCAGAGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAAACTCCGATTTGTTGAGAAA GAGATAAGGAAAGCTAACATCCCAATTATGGACACCGGTGAAAACCCAGAGGTGCCCTTCCCTCGGGACA TGATCGACTTAGAGGCCAATTTCGAGAAGATTGAAAATGAACTGAAGGAAATCAACACTAACCAGGAAGC TCTGAAGAGAAACTTCCTGGAACTGACTGAATTAAAATTTATCCTGCGAAAAACCCAGCAGTTTTTCGAT GAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCATCACTCTTGGAGCCAAACGAGATGGGAAGAGGCG CACCCTTACGACTTGGCTTTGTGGCTGGTGTGATTAACCGGGAGCGGATCCCGACCTTTGAGCGCATGCT TTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAAGCTGAAATCGAAAACCCGCTGGAGGATCCTGTG ACTGGCGACTACGTGCACAAGTCTGTGTTCATCATTTTTTTCCAAGGCGATCAGCTGAAAAACAGGGTCA AGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCCCTGCCCTGAGACTCCACAGGAAAGGAAAGAAAT GGCTTCCGGAGTCAATACCAGGATCGACGACCTCCAAATGGTTCTGAATCAGACGGAGGACCACCGCCAG AGGGTTCTGCAGGCAGCTGCTAAGAACATCCGCGTCTGGTTCATCAAGGTGCGGAAGATGAAGGCTATCT ACCATACCCTGAACCTGTGCAATATAGACGTGACCCAGAAGTGCCTGATCGCAGAAGTCTGGTGCCCTGT CACTGACCTGGACTCCATCCAGTTTGCACTACGAAGAGGCACGGAACACAGTGGGTCCACTGTCCCCTCC ATTCTGAACAGGATGCAGACAAACCAGACACCGCCGACATATAATAAAACCAACAAGTTCACGCACGGCT TCCAGAACATAGTAGATGCTTATGGGATCGGCACTTACCGAGAGATTAACCCAGCTCCGTACACTGTCAT CACCTTCCCCTTTCTGTTTGCTGTGATGTTCGGAGATTTTGGGCACGGCATTTTGATGACGCTGTTTGCT GTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCCAGAAGCACGAGAATGAGATGTTTAGCATGGTGT TCAGCGGCCGGTACATTATTCTTCTGATGGGACTGTTCTCCATCTATACTGGACTCATCTACAATGACTG CTTTTCCAAGTCTCTGAATATCTTTGGGTCATCGTGGAGTGTACGGCCAATGTTCACTCAAGGGAACTGG ACGGAGGAGACGCTTCTGGGGAGTTCTGTTCTCCAGTTGAACCCCGCTATCCCTGGAGTTTTTGGTGGCC CGTACCCGTTTGGCATTGATCCGATTTGGAACATTGCAACCAACAAGCTGACCTTCCTCAACTCCTTCAA GATGAAGATGTCTGTTATCCTCGGCATCATCCACATGCTGTTTGGAGTCAGCCTGAGCCTTTTCAACCAT ATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCTTTATTCCTGAGATCATCTTCATGTCCTCGTTGT TTGGCTACCTGGTCATCCTTATCTTTTACAAGTGGACAGCCTACGATGCCCACTCGTCTAGGAATGCCCC GAGCCTCCTGATCCACTTCATAAACATGTTCCTCTTCTCCTACCCAGAGTCTGGTAATGCAATGCTGTAC TCTGGACAGAAAGGAATTCAGTGTTTCCTCATCGTGGTGGCGATGCTCTGCGTCCCTTGGATGCTTCTGT TTAAGCCGCTGATCCTTCGCCATCAGTACCTGAGGAAGAAGCATTTGGGAACTCTCAACTTTGGTGGGAT CAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAAATTATTCAGCATGACCAGCTTTCCACCCATTCA GAGGACGCAGAAGAGTTTGACTTTGGCGACACCATGGTCCACCAGGCCATCCACACCATTGAGTACTGCT TGGGTTGTATCTCCAACACCGCGTCGTACCTGCGACTCTGGGCCCTCAGCCTGGCCCACGCACAGCTCTC TGAAGTACTTTGGACCATGGTGATCCACATTGGCCTGCACGTCCGGAGTTTGGCCGGGGGTCTAGGGCTG TTTTTCATCTTCGCCGCCTTTGCCACGCTGACCGTGGCTATCCTGCTGATCATGGAGGGCCTCTCGGCCT TCCTCCACGCGCTGCGGTTACACTGGGTGGAGTTCCAGAACAAATTCTACACTGGGACTGGCTTCAAGTT CCTCCCCTTCTCCTTTGAGCACATTCGGGAAGGGAAGTTTGATGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243050
Insert Size	2499 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243050.1, NP_001229979.1
RefSeq Size	4001 bp
RefSeq ORF	2499 bp
Locus ID	11975
UniProt ID	Q9Z1G4
Gene Summary	Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.

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