Kcnh2 (NM_001294162) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI326795; ERG1; LQT; Lqt2; M-erg; Merg1; merg1a; merg1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228999 representing NM_001294162 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATTCCAGCCGGGAAGGAGAGCAGGACAGGGGCTCTGCAGCCCAGGGCCCAGAAAGGCCGGGTGA GGCGGGCAGTGCGCATCTCCAGCCTGGTGGCCCAGGAGGTCCTGTCCTTGGGAGCAGATGTGCTGCCTGA GTATAAGCTGCAGGCCCCAAGAATCCACCGCTGGACCATCCTCCACTACAGCCCCTTCAAGGCGGTGTGG GACTGGCTCATCCTGCTGCTGGTCATCTACACGGCAGTCTTCACACCGTACTCGGCCGCCTTCCTGCTGA AGGAGACTGAAGATGGGTCCCAAGCCCCTGACTGTGGCTACGCCTGCCAGCCCCTGGCTGTAGTGGACCT CATCGTGGACATCATGTTCATTGTGGACATCCTTATCAATTTCCGTACCACCTATGTTAATGCCAACGAG GAGGTGGTTAGCCATCCTGGCCGCATTGCTGTGCACTACTTCAAGGGCTGGTTTCTCATTGACATGGTGG CTGCCATCCCCTTCGACCTGCTCATCTTTGGCTCTGGCTCCGAGGAGCTGATCGGGCTGCTGAAAACTGC GAGGCTGCTGCGGCTGGTGCGCGTGGCTCGGAAGCTGGACCGCTACTCGGAGTACGGGGCAGCAGTGCTC TTCCTGCTCATGTGCACCTTCGCCCTCATTGCGCACTGGTTGGCCTGCATCTGGTACGCGATCGGCAACA TGGAACAGCCTCACATGGACTCACACATCGGCTGGCTGCACAACTTGGGCGACCAGATCGGCAAGCCCTA CAACAGCAGCGGCCTGGGAGGCCCCTCCATCAAGGACAAGTATGTCACAGCGCTCTACTTCACCTTCAGC AGCCTCACCAGCGTGGGCTTTGGCAATGTCTCTCCCAACACCAACTCAGAGAAGATCTTCTCCATCTGTG TCATGCTCATTGGCTCCCTCATGTATGCCAGCATCTTCGGCAACGTGTCCGCCATCATCCAGCGGCTGTA CTCGGGCACGGCCCGCTACCACACACAGATGCTCCGGGTGCGGGAGTTTATCCGCTTTCACCAGATTCCC AACCCATTACGCCAGCGCCTCGAGGAGTACTTCCAGCATGCTTGGTCCTACACCAATGGCATCGACATGA ACGCGGTGCTGAAGGGCTTCCCTGAGTGCCTCCAGGCTGACATCTGCCTACACCTGAACCGCTCGCTGCT GCAGCATTGCAAGCCATTCCGAGGGGCCACTAAAGGCTGCCTGAGGGCGCTGGCTATGAAGTTCAAGACC ACACATGCACCACCAGGGGACACACTAGTGCACGCCGGGGACCTGCTTACTGCCCTCTACTTCATCTCCA GGGGCTCCATCGAGATCCTGCGGGGTGATGTCGTGGTGGCCATCTTGGGGAAGAACGACATCTTTGGAGA GCCTCTGAACCTATATGCCCGTCCTGGAAAGTCCAATGGGGATGTGCGGGCCCTCACGTACTGTGACCTG CACAAGATCCATCGTGATGACTTGCTGGAGGTGTTGGACATGTATCCCGAGTTCTCAGATCACTTTTGGT CTAGCCTGGAGATCACCTTCAACCTTCGAGATACCAACATGATTCCTGGCTCCCCAGGCAGTGCAGAACT AGAGAGCGGCTTTAACAGGCAACGCAAGCGCAAACTGTCATTCCGCAGGCGTACAGACAAGGACACAGAG CAGCCAGGGGAGGTGTCAGCCCTGGGGCAAGGCCCTGCCCGAGTTGGGCCAGGGCCGAGTTGCCGGGGAC AGCCGGGAGGGCCATGGGGGGAGAGCCCATCCAGCGGCCCTTCCAGCCCAGAGAGCAGTGAAGATGAGGG CCCTGGTCGCAGCTCCAGTCCCCTCCGCCTGGTGCCCTTCTCCAGCCCCAGGCCTCCGGGAGACCCCCCA GGTGGGGAGCCTTTGACAGAGGATGGGGAGAAAAGTGACACCTGTAATCCCTTGTCAGGTGCCTTCTCTG GGGTGTCCAATATTTTCAGCTTCTGGGGGGACAGTCGGGGGCGCCAGTACCAGGAGTTGCCTCGATGCCC TGCCCCTGCCCCCAGCCTCCTCAACATCCCCTTGTCTAGCCCTGGTCGGCGATCCCGGGGTGATGTGGAG AGCAGGCTGGACGCACTCCAGAGACAGCTGAACAGGCTGGAAACCCGGCTAAGTGCAGACATGGCCACTG TCCTACAGCTGCTACAGAGGCAAATGACCCTGGTCCCTCCTGCCTACAGTGCTGTGACCACCCCTGGGCC CGGCCCCACTTCCGCATCCCCTTTGTTGCCTGTCGGCCCTGTCCCCACTCTCACCCTGGACTCGCTTTCT CAGGTTTCCCAGTTCGTGGCGTTTGAGGAGCTTCCTGCAGGAGCCCCAGAGCTCCCCCAAGATGGCCCCA CTCGACGCCTCTCCCTGCCCGGCCAGCTGGGGGCCCTTACCTCCCAGCCCCTGCACAGACATGGCTCAGA TCCAGGCAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001294162
Insert Size	2463 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001294162.1, NP_001281091.1
RefSeq Size	3193 bp
RefSeq ORF	2463 bp
Locus ID	16511
UniProt ID	O35219
Gene Summary	Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks several exons in the 5' coding region and contains an alternate 5' terminal exon, compared to variant 1. It encodes isoform 2 (also known as Merg1b), which is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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