Zfp27 (NM_011754) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mkr-4; mszf76; Zfp-27
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228995 representing NM_011754 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGACTATAGATTTCAGCAGAGAGGAGTGGCAGCATCTAGATCCTGCTCAAAGGAGTCTCTATC GGGATGTGGTGCAGGAGACCTACAGTCACCTGCGCTCAGTGGAAGAACTACAGCAGATTGGTGACCAGAA AAAGACCTACCAGCAAATAGGACATAAATCAGCGAGTGATATGGTTTTCATCACGAAGACGCTGGGTGCA GAGAGTTGCCATGACTACTCAGGCGTTAGGAAGGTGATTCATGTGAACTCATACATTGTTCTCCCTCCCA AGAGGCCACGTCACTGGGACCCACCTGAGGATGAGCCGAAGCATAGTTCAGATTTACAGACTCATGATGA AAGTAATGGGCTGAAGAGAACTAAAAGGATTACTGAATATGGTAAAATTTCATCCTGTATCAACACTGAA CATATTCTAACAGGAGAGAAATTACCGGACCATAATCAATGTGGAAAAGTCCTCGGTTATAAACAAATAC CTTGTCAATATCAGAAAATTCATACTGGGGAGAAATCATATGAATGTGCTGAATTTGGAAAGATTTTTAC CCAGAAGTCACAGCTCAGAGTACACGTGACGTCTCCTACAGGAGAAAAACTCTACGTGTGCGTCGAATGT GGGAAGGCATGTTCTCAGACGTCAGAGTTCCTCACACATCAAAAAACCCACACCAGGGAGAAGCCCTATA AATGCGGTGACTGTGGAAAGTCCTTTTTCCAAGTATCTTCTCTGTTCAGGCACCGGAGGATCCACACTGG AGAAAAGCTCTACGACTGTAGCCATTGTGGGAAAGGCTTCTCTTACAACTCAGACCTCAGGATACATCAG AAGATCCATACAGGAGAGAAACGCCATGGCTGCGTCGACTGTGGCAAAGCCTTCACACAAAAGTCCACAC TGAGGATGCACCAGAAGATCCACACGGGCGAGAGGGCCTATGTCTGTATTGAGTGTGGACAGGCTTTTAT CCAGAAGACACACCTGGTCGCACACCGAAGAATTCACACTGGAGAGAAGCCATACGCGTGTGACGGCTGT GGCAAAGCCTTCCTTTCCAAGTCCCAACTCCTGGTCCACCAGCGAATTCACTCAAGAGTGAGGCCCTGTG TATCTCTCGACCGTGCGAAACCCTTCAGCAGCGCTCCCAACCTCCTTCCGCGTAAGAAAGTTCAAATGAG AGAGAAATCGTCCATCTGCGCGGAGTGTGGGAAGGCCTTTACCTACAGGTCGGAGCTAATCATTCATCAG AGAACTCACACGGGAGAGAAGCCTTATCAGTGCGGGGACTGTGGCAAAGCCTTCACCCAGAAGTCAGCCC TCACCGTGCACCGAAGAATCCACACGGGGGAGAAGTCGTATGTGTGTGTGAAGTGCGGGCTAGCCTTCGT GCAAAGGGCACACTTGGATGCACACCAGGTGATTCACACTGGAGAAAAACCTTACCAGTGTGGTCATTGT GGGAAATTCTTCACTTCCAAGTCGCAACTCCACGTGCACAAGCGGATTCACACGGGGGAAAAACCCTATG TGTGCAGTAACTGTGGGAAGGCCTTTGCCAACAGGTCAAATCTCATCACACATCAGAAAACTCATACAGG GGAGAAAGCCTATGTCTGTGCAAGGTGTGGGAAAGCATTCACTCAGAGGTCAGACCTGGTTACACACCAG AGGATACATACCGGAGAGAAGCCATATGGGTGTAGCACCTGCGGGAAAGCCTTTACCCAGAAATCACACC TCAGTATACACGAGAAGATTCACACCGGAGAGAGACAGTACGGATGCCGAGACTGTGGGAAAGCCTTCAA CCAGAAATCCATACTCATCGTGCACCAGAAAATCCACACGGGGGAGAAACCGCACGTGTGTGCCGAGTGC GGGCGAGCTTTCATCCGCAAGTCCAACTTCATCACCCATCAGCGCATCCATACTGGCGAGAAACCTTATG GATGCACCGACTGCGGCAAGTCCTTTACCTCCAAGTCGCAGCTCCTGGTGCACCGGCCAATCCACACCGG CGAGAAACCTTACGTGTGTGCCGAGTGTGGGAAAGCCTTTAGCGGCAGGTCAAATCTCAGCAAGCACCAG AAGACTCACACTGGGGAGAAGCCGTACGCGTGTTCAGAGTGTGGGAAGTCCTTCAGGCAGAAGTCAGAGC TGATTACACACCACAGAATCCACACTGGGGAGAAGCCGTACGATTGCGGCGACTGCGGGAAATCCTTCAC TAAGAAATCCCAGCTCCAAGTGCATCAGCGAATTCACACAGGAGAGAAGCCGTACAGATGTGCCGAGTGC GGGAAGGCCTTCACCGATAGGTCCAATTTGAATAAACATCAAACAACACACACCGGAGAGAAACCTTATA AGTGTGTAGTCTGTGGGAAAGGCTTTGTGCAGAAGTCAGTGCTCAGCATTCATGAGAATGTTCACACCTC GGCAGTATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_011754
Insert Size	2460 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011754.2, NP_035884.2
RefSeq Size	3524 bp
RefSeq ORF	2460 bp
Locus ID	22689
UniProt ID	P10077
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 3. All four variants encode the same protein.

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