Postn (NM_001198765) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A630052E07Rik; AI747096; Os; OSF; OSF-2; Osf2; p; Pe; PLF; PN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228982 representing NM_001198765 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCCTCTCCTGCCCTTATATGCTCTGCTGCTGCTGTTCCTGTGTGATATTAACCCTGCAAATGCCA ACAGTTACTATGACAAGGTCCTGGCTCACAGCCGCATCAGGGGTCGGGATCAGGGCCCAAACGTCTGTGC CCTCCAGCAAATTCTGGGCACCAAAAAGAAATACTTCAGCTCCTGTAAGAACTGGTATCAAGGTGCTATC TGCGGGAAGAAAACCACTGTGCTATATGAATGCTGCCCTGGCTATATGAGAATGGAAGGGATGAAAGGCT GCCCCGCAGTGATGCCTATTGACCATGTTTATGGCACGCTGGGCATTGTGGGAGCCACTACCACTCAGCA CTACTCCGATGTCTCGAAGCTGAGAGAAGAGATTGAAGGAAAAGGGTCATACACGTACTTCGCGCCGAGT AACGAGGCTTGGGAGAACCTGGATTCTGACATTCGCAGAGGACTGGAGAACAATGTCAATGTTGAGCTAC TGAATGCCTTACACAGCCACATGGTTAATAAGAGAATGTTAACCAAGGACCTGAAACACGGCATGGTTAT TCCTTCAATGTACAACAATCTGGGGCTTTTTATTAACCATTATCCCAATGGGGTTGTCACTGTGAACTGT GCTCGAGTCATCCATGGGAACCAGATTGCCACAAATGGTGTCGTCCATGTCATTGACCGTGTCCTGACAC AAATTGGTACCTCCATCCAAGACTTCCTTGAAGCAGAAGACGACCTTTCATCATTTAGAGCAGCCGCCAT CACCTCTGACCTCTTGGAGTCCCTTGGAAGAGATGGTCACTTCACGCTCTTTGCTCCCACCAATGAAGCT TTCGAGAAACTGCCACGAGGTGTCCTAGAAAGGATCATGGGAGACAAAGTGGCTTCTGAAGCTCTCATGA AGTACCACATCCTAAATACCCTCCAGTGCTCTGAGGCCATCACTGGAGGAGCCGTGTTTGAGACCATGGA AGGAAACACTATTGAGATAGGGTGCGAAGGGGACAGTATCTCCATTAACGGAATCAAGATGGTGAACAAG AAAGACATTGTGACTAAGAATGGTGTCATCCACCTGATTGATGAAGTCCTCATTCCTGATTCTGCCAAAC AAGTTATTGAGCTGGCTGGAAAACAGCAAACCACTTTCACCGACCTGGTAGCCCAATTAGGCTTGGCATC CTCTCTGAAGCCAGATGGAGAGTACACCTTATTAGCACCTGTGAACAATGCGTTCTCTGATGACACTCTG AGCATGGACCAACGCCTTCTTAAGCTAATTCTGCAAAATCACATATTGAAAGTAAAAGTTGGCCTTAGCG ACCTCTACAATGGACAGATACTGGAAACCATTGGAGGCAAACAACTCCGAGTCTTTGTGTATCGGACGGC TATCTGCATAGAAAACTCATGCATGGTGAGAGGAAGCAAGCAGGGAAGGAATGGTGCCATTCACATATTC CGAGAAATCATCCAACCAGCAGAGAAATCCCTGCACGACAAGCTGCGGCAAGACAAGCGCTTTAGCATCT TCCTCAGCCTCCTTGAAGCTGCAGATTTGAAAGATCTCCTGACACAGCCCGGAGATTGGACCTTGTTTGC ACCAACCAATGATGCCTTCAAGGGAATGACTAGCGAAGAAAGGGAGCTTCTGATTGGGGATAAAAATGCT CTCCAAAACATCATTCTTTATCACCTGACCCCAGGGGTTTATATTGGAAAGGGATTCGAACCCGGAGTCA CTAATATCCTGAAGACCACACAGGGAAGCAAAATCTATCTGAAAGGAGTAAACGAAACGCTTCTAGTGAA TGAGTTGAAGTCCAAAGAATCTGACATCATGACGACAAATGGTGTCATCCACGTCGTGGACAAACTCCTC TATCCAGCAGATATTCCAGTTGGAAATGATCAGCTCTTGGAATTACTGAACAAACTGATAAAATACATCC AAATCAAGTTTGTTCGTGGCAGCACCTTCAAAGAAATCCCCATGACTGTCTATACAACTAAAATTATAAC CAAAGTCGTGGAACCAAAAATTAAAGTCATTCAAGGCAGTCTTCAGCCTATTATCAAAACGGAAGGACCT GCAATGACGAAGATCCAAATTGAAGGTGATCCCGACTTCAGGCTGATTAAAGAAGGCGAAACGGTGACAG AAGTGATCCACGGAGAGCCAGTCATTAAAAAGTACACCAAAATCATAGATGGAGTTCCTGTTGAAATAAC TGAAAAACAGACTCGGGAAGAACGAATCATTACAGGTCCTGAGATAAAATATACCAGGATTTCCACAGGA GGTGGAGAAACAGGAGAGACCTTGCAGAAATTCTTGCAAAAAGACACACCTGCAAAGAAGATACCAGCCA ACAAAAGGGTTCAAGGGCCTAGAAGACGATCAAGAGAAGGCCGTTCTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198765
Insert Size	2433 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198765.1, NP_001185694.1
RefSeq Size	3226 bp
RefSeq ORF	2433 bp
Locus ID	50706
UniProt ID	Q62009
Gene Summary	This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (2) contains an alternate in-frame exon, and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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