Ptpdc1 (NM_001301781) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI843923; AW456874; Naa-1; Ptpcd1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228979 representing NM_001301781 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCAGGAGTCTTGCCACAGAATGAAGATCCATATTCCACGTTGGTGAATAGCAGTGGGCATGCTG CACACATGGATGGTGAGGAGAAAATGCTTATCTTGAGACATCTTACGGACCCAGTCTGGATGTGGAGCCG TGTGTTTTGCCGCCTAGAGCTCGCTGAGCCCCTCAGAGAGTCCATCCTGCTCGTCACTGCATCTGCGATG CTGAAGCTGCGGTTTCTCACTCGGCTGTCTTCTGGGCTCTCCTGCTGCATTCTTGAAAATTCAGGACGCC CAGCACCAAAATACACGAAAGTGGGAGAACGTTTGCGGCATGTCATCCCTGGGCACATGGCATGTTCCAT GGCGTGTGGTGGGAGAGCGTGTAAGTATGAGAACCCAGCCCGCTGGAGTGAGCAGGAGCAAGCCATTAAG GGAGTATACTCATCCTGGGTCACTGACAATATCCTGGCCATGGCTCGTCCGTCCTCCGAGCTGCTGGAGA AGTATCGCATCATCGAGCAGTTCCTCGGTCAAGGCATAAAAACAATAATCAACCTGCAGCGCCCTGGAGA GCATGCCAGCTGTGGGAGCGCTCTGGAACAAGAAAGTGGTTTCACCTACCTTCCTGAGGCATTCATGGAG GCTGGCATTTATTTCTACAACTTTGGGTGGAAGGATTATGGCGTGGCTTCCCTCACTGCCATCTTGGACA TGGTAAAGGTGATGACATTTGCCTTACAGGAAGGGAAAGTGGCTGTCCACTGCCACGCGGGGCTCGGCCG CACAGGTGTGCTAATAGCATGTTATTTAGTTTTTGCAACAAGAATGACTGCTGACCAAGCAATTATATTT GTTCGGGCAAAACGCCCCAATTCCATACAAACAAGAGGACAACTGCTCTGTGTACGGGAATTCACGCAGT TCCTGGCTCCACTCCGTAATATTTTCTCTTGCTGTGACCCCAAAGCACATGCTGTTACCTTAGCCCAGTA CCTGATTCGCCAGCGACATCTGCTTCATGGCTACGAGGCACGACTTCTGAAGTACGTGCCGAAGATCATC CACCTGGTTTGCAAACTGCTGCTTGACTTGGCTGAGAACCGGCCAGTGGTGATGAAGAGCATGCTAGAAG GACCTGTGCTCTCTGCTGAAATCGAAAAGACCGTGTCTGAGATGGTCACGCTGCAGCTAGATCAAGAGTT ACTCAGGCAAAACAGTGATGTGCCCGACCCGTTTAACCCCACTGCAGAGGTGGCCGAGTTCGAGAATCAG GATGTGATTCTCTCTACTGAGCAGGAGTTTGACCCCCTCTGGAAGAGGAGGGATATTGAGTGCCTTCAGC CCCTGACACATCTGAAAAGACAGCTCAGCTACAGCGACTCAGACTTAAAGAGGGCCAAAGCCATCCTGGA ACAAGGGGAGACACCTTGGACAGTGCCTGCCCAGGAGTTGCTAGACCACAGTCTCCAGCATCAGAAGCCC ACGAGCCATTGCTACATGCCACCAACTCCAGAGCTGGGTTTTAATAAGGAGGCACTGGTCCAAAATACCT TTTCTTTCTGGACTCCGTCAAAGTGTGGAGGCTTAGAAGGACTGAAGGATGAGGGATCGCTGCTTTTGTG CAGGAAGGACATTCCAAAGGAAGTACAGCGGAGCAGAACCTTCTCTGTGGGTGTTTCTTGTTCACACAAT CCTGGAGAGCCAGTGCCGCCCAACTTCACAAGTATCCATAAGGATCCAGAGCAGGTCACCCATTGCCGGT GTGAGGCTCCTGGTGGCTGGGTCCCGGGCCCTGTTCACGAGATGGTCAGGAGCCCCTGTAGCCCTCTGAA CTGTGGCTCCAGTCCTAAGGCGCAGTTCCCACACGGACAGGAAACCCAAGACAGCACAGACCTGTCTGAG GCAGTGCCGCATGCTGGTCTGCAGCCTGAACTGAGTGCTGAAGCAAGGAGAATACTGGCGGCCAAAGCCC TGGCAAACTTAAATGAGTTTGTTGAAAAGGAGGAAGTGAAAAGGAAAGTGGAGATGTGGCAGAAAGAACT AAATTCCCGGGAGGAAGCCTGGGAACGAATATGCGGCGAGAGAGACCCTTTCATCTTGTGCAGTTTGATG TGGTCTTGGGTTGAGCAACTGAAAGAGCCTGTAATCACCAAAGAGGATGTGGACATGCTGGTCGACAGAC AAGCAGATGCTGCCGAAGCCCTGTTTTTACTAGAGAAGGGGCAGTACCAGACCATCCTCTGTGTGCTGCA CTGCATAGTGAGCCTGCAGACGCTTCCCATGGAGGTGGAGGAAGCTTGCCTTTTACATGCCATTAAAGCT TTCACCAAGGTTAATTTTGATTCTGAAAACGGACCAATAGTTTACGACACCTTGAAAAAAATATTTAAGC ACACGTTGGAAGAAAAGAGGAAAATGGCAAAAGACAGCCTCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301781
Insert Size	2427 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301781.1, NP_001288710.1
RefSeq Size	4612 bp
RefSeq ORF	2427 bp
Locus ID	218232
UniProt ID	Q6NZK8
Gene Summary	The protein encoded by this gene is a centrosomal mitotic phosphatase. This protein has been implicated in centrosomal duplication and cytokinesis. In addition, knockdown of expression levels in non-cycling cells results in extra long cilia, suggesting that this protein may function in regulating cilia length independent of a function in cell cycle control. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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