Ampd2 (NM_001289719) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200014F01Rik; AI552571; Ampd-2; m4521Dajl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228954 representing NM_001289719 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAGAAGCTCGAAGCGGTCTGGGGGCCTCTCCGCTACAGTCTGCCCGATCCCTGCCAGGCAACG CCCCTTGCCTCAAGCACTTCCCGCTTGACCTGCGCACGTCTATGGATGGCAAATGCAAGGAGATCGCTGA GGAGTTGTTCAGCCGCTCACTGGCTGAGAGTGAGCTTCGTAGCGCCCCTTATGAGTTCCCAGAGGAAAGC CCCATCGAGCAGCTAGAAGAACGGAGGCAGCGGCTGGAGCGCCAGATCAGCCAGGATGTCAAGCTGGAGC CAGATATTCTTCTTCGAGCCAAGCAAGATTTCCTGAAGACAGACAGCGACTCAGACTTACAGCTGTACAA GGAGCAAGGAGAGGGACAGGGTGACAGGGGTCTTTGGGAACGTGATGTGGTATTGGAACGGGAATTTCAG CGGGTCATCATCTCTGGGGAGGAGAAGTGTGGGGTGCCATTCACAGACCTCTTAGACGCAGCCAAAAGTG TGGTTCGGGCACTTTTCATCCGGGAGAAGTACATGGCCCTATCACTGCAGAGCTTCTGTCCCACCACCCG CCGTTACCTGCAGCAGCTGGCTGAGAAGCCCCTGGAGACTCGAACTTATGAGCAGAGTCCTGATACCCCT GTATCTGCTGATGCCCCAGTGCATCCCCCTGCACTGGAGCAGCACCCGTATGAGCACTGTGAGCCAAGCG CCATGCCTGGGGACCTGGGCTTGGGTCTGCGCATGGTGCGTGGTGTGGTGCACGTCTACACCCGCAGGGA CCCTGATGAGCACTGTCCGGAGGTGGAGCTTCCATACCCTGACCTACAGGAATTTGTAGCTGACGTCAAT GTGCTGATGGCCCTGATCATCAATGGTCCCATAAAGTCATTCTGCTACCGCCGGCTGCAGTACCTGAGCT CCAAATTCCAGATGCACGTTTTGCTCAATGAGATGAAGGAGCTCGCTGCTCAGAAGAAAGTGCCACACCG GGACTTCTACAATATCCGTAAGGTGGACACACACATCCACGCCTCGTCCTGCATGAACCAGAAACATCTA CTGCGCTTCATCAAGCGGGCCATGAAGCGGCACCTGGAGGAGATTGTGCATGTGGAACAGGGCCGCGAGC AGACGCTGAGAGAAGTCTTCGAGAGCATGAACCTCACTGCCTACGACTTAAGTGTGGACACGCTGGATGT GCATGCGGACAGGAATACCTTTCATCGATTTGACAAATTCAATGCCAAATACAACCCTATTGGGGAGTCT GTTCTCCGAGAGATCTTCATTAAAACCGACAACAAGATTTCTGGGAAGTACTTTGCTCACATCATCAAGG AGGTGATGGCAGACTTGGAGGAGAGCAAATACCAGAATGCAGAGCTCCGGCTGTCCATCTACGGGCGTTC GAGGGATGAGTGGGACAAGCTGGCACGCTGGGCAGTGAACCACAAAGTGCACTCTCCCAATGTCCGCTGG CTGGTGCAGGTGCCCCGCCTCTTCGATGTGTACCGCACCAAGGGCCAGCTGGCCAACTTCCAAGAGATGC TGGAGAACATCTTTCTGCCCCTGTTTGAGGCTACTGTGCACCCTGCCAGCCACCCGGAGCTGCACCTCTT TCTGGAGCACGTGGATGGTTTTGATAGCGTGGATGATGAGTCCAAGCCAGAGAACCACGTCTTCAACCTG GAGAGTCCCCTCCCAGAAGCTTGGGTGGAGGAGGACAACCCTCCCTATGCCTACTACCTGTACTACACCT TCGCTAACATGGCTATGTTGAACCATCTGCGCAGGCAGAGAGGTTTCCACACGTTCGTGCTGAGGCCGCA CTGTGGGGAGGCCGGGCCCATCCACCACCTGGTATCAGCCTTCATGCTGGCCGAGAACATCTCCCACGGG CTGCTCCTGCGCAAGGCCCCCGTCCTGCAGTACCTGTATTACCTGGCTCAGATCGGCATCGCCATGTCCC CGCTCAGCAACAACAGCCTGTTCCTCAGCTACCACCGGAACCCTCTCCCTGAGTACTTGTCCCGTGGCCT CATGGTCTCGCTGTCCACAGATGATCCCTTGCAGTTCCACTTCACCAAGGAGCCCCTGATGGAGGAGTAC AGCATCGCCACCCAGGTGTGGAAGCTCAGCTCCTGCGATATGTGCGAGCTGGCCCGTAACAGCGTGCTCA TGAGTGGCTTCTCTCACAAGGTGAAAAGCCACTGGCTGGGACCCAACTATACCAAGGAGGGCCCTGAGGG CAATGATATCCGCCGTACCAACGTGCCAGACATCCGAGTGGGCTACCGCTATGAGACGCTATGCCAGGAG CTGGCACTTATCACACAGGCCGTCCAAAGTGAGATGCTGGAGACCATCCCAGAGGAAGTGGGCATTGTCA TGAGCCCAGGGCCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289719
Insert Size	2397 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289719.1, NP_001276648.1
RefSeq Size	3608 bp
RefSeq ORF	2397 bp
Locus ID	109674
UniProt ID	Q9DBT5
Gene Summary	AMP deaminase plays a critical role in energy metabolism. Catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform a. Variants 2 and 3 both encode the same isoform (b).

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